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VPS35L (NM_020314) Human Tagged ORF Clone

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SKU
RC208420
C16orf62 (Myc-DDK-tagged)-Human chromosome 16 open reading frame 62 (C16orf62)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $882.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol VPS35L
Synonyms C16orf62; EC97
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208420 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGTCTTTCCTTGGCACTCCAGGAATAGGAACTACAAAGCTGAATTTGCATCATGCCGACTGGAGG
CTGTACCATTGGAGTTTGGGGACTATCACCCTCTGAAACCCATAACTGTCACAGAGTCAAAGACAAAGAA
AGTGAACCGGAAAGGAAGCACTTCTTCCACGTCCTCCTCCTCCTCCAGCTCCGTGGTGGACCCGCTGAGC
AGCGTCCTCGATGGGACTGACCCCCTCTCCATGTTTGCAGCCACTGCTGACCCCGCAGCCTTGGCAGCTG
CCATGGACAGCTCCAGAAGGAAACGTGATAGAGATGATAACTCCGTTGTAGGATCGGATTTTGAGCCTTG
GACCAACAAACGGGGAGAAATCCTTGCCCGGTACACCACTACCGAAAAGCTGTCTATTAATCTGTTTATG
GGATCTGAAAAAGGCAAAGCTGGGACTGCCACATTGGCAATGTCAGAGAAGGTGCGGACCCGGCTGGAGG
AGCTGGATGACTTTGAGGAGGGTTCCCAAAAGGAGCTGTTGAACTTGACTCAGCAGGATTACGTGAACCG
CATAGAGGAGCTCAACCAATCGCTGAAGGATGCCTGGGCCTCAGACCAGAAAGTGAAGGCTCTAAAAATA
GTCATCCAGTGTTCAAAGCTTCTTTCAGACACCAGTGTTATTCAGTTCTACCCAAGCAAATTTGTCCTTA
TCACCGACATACTTGATACATTTGGAAAGCTCGTGTACGAGCGCATCTTTTCCATGTGTGTGGATAGCCG
CAGCGTCTTACCAGATCACTTTTCTCCAGAGAATGCAAATGACACGGCCAAGGAAACATGCCTAAATTGG
TTTTTCAAGATTGCCTCCATCAGGGAACTCATTCCAAGATTTTACGTGGAGGCATCCATCCTGAAATGTA
ACAAATTCCTCTCCAAAACGGGAATTTCAGAGTGCCTGCCCCGGTTGACATGCATGATCAGAGGGATCGG
AGACCCACTAGTGTCGGTGTATGCCCGTGCCTACCTGTGCCGGGTGGGAATGGAAGTGGCCCCACATCTC
AAAGAAACCCTAAATAAGAACTTTTTTGACTTCCTCCTTACGTTCAAACAGATTCATGGGGATACGGTCC
AGAACCAGCTGGTGGTCCAAGGAGTGGAGCTCCCATCTTACCTCCCCTTGTACCCGCCTGCCATGGACTG
GATCTTCCAGTGCATCTCCTACCATGCCCCCGAGGCTCTGCTGACCGAGATGATGGAAAGGTGTAAGAAA
CTAGGAAACAATGCCTTGCTGTTGAATTCTGTGATGTCTGCCTTCCGGGCTGAGTTCATCGCCACAAGGT
CTATGGATTTCATTGGCATGATTAAAGAGTGTGATGAATCTGGTTTCCCCAAGCATCTTCTTTTTCGATC
ACTGGGATTAAACTTGGCCTTGGCTGATCCTCCTGAGAGTGACCGACTTCAGATTCTCAACGAAGCTTGG
AAAGTCATCACTAAGCTGAAGAACCCACAGGACTACATTAATTGTGCCGAAGTGTGGGTGGAATACACCT
GCAAGCATTTCACGAAACGAGAGGTGAATACCGTTTTGGCAGATGTCATCAAGCACATGACTCCAGATCG
TGCATTTGAAGATTCCTACCCCCAGCTTCAGTTAATAATTAAGAAAGTTATTGCCCACTTCCATGACTTC
TCAGTTCTTTTCTCAGTGGAAAAATTTCTGCCGTTTCTGGACATGTTCCAAAAAGAGAGTGTGCGGGTGG
AGGTTTGCAAATGCATCATGGACGCCTTTATCAAGCATCAACAAGAGCCCACCAAGGACCCGGTCATCTT
GAATGCCCTTTTGCATGTTTGCAAGACCATGCATGACTCTGTGAATGCACTCACTCTTGAGGATGAGAAA
AGAATGCTGTCATATTTGATTAATGGATTTATAAAAATGGTTTCCTTTGGCCGTGATTTTGAACAACAGC
TGAGTTTTTATGTTGAGTCCAGGTCGATGTTTTGCAATCTGGAGCCTGTTCTTGTGCAGTTGATTCATAG
TGTGAACCGGTTGGCAATGGAGACAAGAAAAGTAATGAAAGGAAATCATTCCAGAAAGACAGCTGCATTT
GTCCGGGCCTGTGTTGCCTACTGCTTCATCACCATCCCCTCCCTGGCGGGCATCTTCACACGTCTCAATC
TCTACCTGCATTCTGGTCAGGTGGCCTTGGCCAACCAGTGCCTCTCCCAAGCTGATGCTTTTTTCAAAGC
CGCTATAAGCCTTGTTCCGGAAGTTCCAAAGATGATTAATATTGATGGGAAGATGCGGCCATCGGAATCG
TTCCTTCTGGAATTCCTCTGCAATTTCTTTTCTACTTTATTAATAGTTCCGGATCATCCTGAACATGGGG
TCCTGTTTCTTGTTCGAGAGCTTCTCAACGTGATCCAGGACTACACCTGGGAGGACAACAGCGATGAGAA
AATCCGCATCTACACCTGCGTCCTGCATCTCCTCTCCGCCATGAGCCAGGAGACGTACCTTTACCACATA
GACAAAGTGGACTCCAACGACAGCCTCTACGGGGGAGACTCCAAGTTCCTGGCAGAAAACAACAAGCTGT
GTGAGACGGTGATGGCTCAGATCCTAGAGCATCTGAAAACCCTGGCCAAGGACGAGGCCCTGAAGCGCCA
GAGCTCGTTGGGCCTTTCCTTCTTTAACAGCATCTTGGCCCATGGGGACCTACGCAACAACAAGCTCAAC
CAGCTCTCCGTCAACCTGTGGCACCTGGCACAGAGGCACGGCTGTGCAGACACCAGGACCATGGTGAAAA
CGCTAGAATACATCAAGAAGCAAAGCAAACAACCAGACATGACTCATCTGACGGAGCTGGCCCTCAGACT
CCCTCTGCAAACAAGGACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208420 protein sequence
Red=Cloning site Green=Tags(s)

MAVFPWHSRNRNYKAEFASCRLEAVPLEFGDYHPLKPITVTESKTKKVNRKGSTSSTSSSSSSSVVDPLS
SVLDGTDPLSMFAATADPAALAAAMDSSRRKRDRDDNSVVGSDFEPWTNKRGEILARYTTTEKLSINLFM
GSEKGKAGTATLAMSEKVRTRLEELDDFEEGSQKELLNLTQQDYVNRIEELNQSLKDAWASDQKVKALKI
VIQCSKLLSDTSVIQFYPSKFVLITDILDTFGKLVYERIFSMCVDSRSVLPDHFSPENANDTAKETCLNW
FFKIASIRELIPRFYVEASILKCNKFLSKTGISECLPRLTCMIRGIGDPLVSVYARAYLCRVGMEVAPHL
KETLNKNFFDFLLTFKQIHGDTVQNQLVVQGVELPSYLPLYPPAMDWIFQCISYHAPEALLTEMMERCKK
LGNNALLLNSVMSAFRAEFIATRSMDFIGMIKECDESGFPKHLLFRSLGLNLALADPPESDRLQILNEAW
KVITKLKNPQDYINCAEVWVEYTCKHFTKREVNTVLADVIKHMTPDRAFEDSYPQLQLIIKKVIAHFHDF
SVLFSVEKFLPFLDMFQKESVRVEVCKCIMDAFIKHQQEPTKDPVILNALLHVCKTMHDSVNALTLEDEK
RMLSYLINGFIKMVSFGRDFEQQLSFYVESRSMFCNLEPVLVQLIHSVNRLAMETRKVMKGNHSRKTAAF
VRACVAYCFITIPSLAGIFTRLNLYLHSGQVALANQCLSQADAFFKAAISLVPEVPKMINIDGKMRPSES
FLLEFLCNFFSTLLIVPDHPEHGVLFLVRELLNVIQDYTWEDNSDEKIRIYTCVLHLLSAMSQETYLYHI
DKVDSNDSLYGGDSKFLAENNKLCETVMAQILEHLKTLAKDEALKRQSSLGLSFFNSILAHGDLRNNKLN
QLSVNLWHLAQRHGCADTRTMVKTLEYIKKQSKQPDMTHLTELALRLPLQTRT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020314
ORF Size 2889 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020314.7
RefSeq Size 3894 bp
RefSeq ORF 2892 bp
Locus ID 57020
UniProt ID Q7Z3J2, E7EWW0
Cytogenetics 16p12.3
MW 109.6 kDa
Summary Acts as component of the retriever complex. The retriever complex is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos such as integrin alpha-5/beta-1 (ITGA5:ITGB1) (PubMed:28892079). The recruitment of the retriever complex to the endosomal membrane involves CCC and WASH complexes (PubMed:28892079). In the endosomes, drives the retrieval and recycling of NxxY-motif-containing cargo proteins by coupling to SNX17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling (PubMed:28892079). Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association with the CCC complex and cooperation with the WASH complex on early endosomes. Seems not to be required for CCC complex stability (PubMed:25355947).UniProtKB/Swiss-Prot Function
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"NM_020314" in other vectors (5)
SKU Description Size Price
RC208420L3 Lenti ORF clone of Human chromosome 16 open reading frame 62 (C16orf62), Myc-DDK-tagged 10 ug
$1,182.00
RC208420L4 Lenti ORF clone of Human chromosome 16 open reading frame 62 (C16orf62), mGFP tagged 10 ug
$1,182.00
RG208420 C16orf62 (tGFP-tagged) - Human chromosome 16 open reading frame 62 (C16orf62) 10 ug
$1,082.00
SC113163 C16orf62 (untagged)-Human chromosome 16 open reading frame 62 (C16orf62) 10 ug
$1,130.00
SC318417 C16orf62 (untagged)-Human chromosome 16 open reading frame 62 (C16orf62) 10 ug
$1,243.00

Plasmid Map for RC208420

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208420
Circular map for RC208420

file-alt Citations

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