KCNK12 (NM_022055) Human Tagged ORF Clone

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SKU
RC208379
KCNK12 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 12 (KCNK12)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KCNK12
Synonyms K2p12.1; THIK-2; THIK2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208379 representing NM_022055
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTCCCGCAGCCCCCGGCCCCCGCCCCGCCGTAGCCGCCGCCGCCTGCCGCGCCCCTCCTGCTGCT
GCTGCTGCTGCCGCCGTTCGCACCTCAACGAGGACACCGGCCGCTTCGTGCTGCTGGCGGCGCTCATCGG
CCTCTACCTGGTGGCGGGTGCCACAGTCTTCTCGGCGCTCGAGAGCCCCGGCGAGGCGGAGGCGCGGGCG
CGCTGGGGCGCCACGCTGCGCAACTTCAGCGCTGCGCACGGCGTGGCCGAGCCAGAGCTGCGCGCCTTCC
TCCGGCACTACGAGGCCGCGCTGGCCGCCGGCGTCCGCGCCGACGCGCTGCGCCCGCGCTGGGACTTCCC
CGGCGCCTTCTACTTCGTGGGCACCGTGGTGTCAACCATAGGTTTCGGCATGACCACCCCCGCGACGGTG
GGCGGGAAGGCCTTCCTCATCGCCTACGGGCTGTTCGGCTGCGCTGGGACCATCCTGTTCTTCAACCTCT
TCCTGGAGCGCATCATCTCGCTGCTGGCCTTCATCATGCGCGCCTGCCGGGAGCGCCAGCTGCGCCGCAG
CGGCCTGCTGCCCGCCACCTTCCGCCGCGGCTCCGCGCTCTCGGAGGCCGACAGCCTGGCGGGCTGGAAG
CCCTCGGTGTACCACGTGCTGCTCATCCTGGGCCTGTTCGCCGTGCTGCTGTCCTGCTGCGCCTCGGCCA
TGTACACCAGCGTGGAGGGCTGGGACTACGTGGACTCGCTCTACTTCTGCTTCGTCACCTTCAGCACCAT
CGGCTTCGGGGACCTGGTGAGCAGCCAGCACGCCGCCTACCGGAACCAGGGGCTCTACCGCCTGGGCAAC
TTCCTCTTCATCCTGCTCGGCGTGTGCTGCATTTACTCGCTCTTCAACGTCATCTCCATCCTCATCAAGC
AGGTGCTCAACTGGATGCTGCGCAAGCTGAGCTGCCGCTGCTGCGCGCGCTGCTGCCCGGCTCCTGGCGC
GCCCCTGGCCCGGCGCAATGCCATCACCCCAGGCTCCCGGCTGCGCCGCCGCCTGGCCGCGCTCGGCGCC
GACCCCGCGGCCCGCGACAGCGACGCCGAGGGCCGCCGCCTCTCGGGCGAGCTCATCTCCATGCGCGACC
TCACGGCCTCCAACAAGGTGTCGCTGGCGCTGCTGCAGAAGCAGCTGTCGGAGACGGCCAACGGCTACCC
GCGCAGCGTGTGCGTCAACACGCGCCAGAACGGCTTCTCGGGCGGCGTGGGCGCGCTGGGCATCATGAAC
AACCGGCTGGCCGAGACCAGCGCCTCCAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208379 representing NM_022055
Red=Cloning site Green=Tags(s)

MSSRSPRPPPRRSRRRLPRPSCCCCCCRRSHLNEDTGRFVLLAALIGLYLVAGATVFSALESPGEAEARA
RWGATLRNFSAAHGVAEPELRAFLRHYEAALAAGVRADALRPRWDFPGAFYFVGTVVSTIGFGMTTPATV
GGKAFLIAYGLFGCAGTILFFNLFLERIISLLAFIMRACRERQLRRSGLLPATFRRGSALSEADSLAGWK
PSVYHVLLILGLFAVLLSCCASAMYTSVEGWDYVDSLYFCFVTFSTIGFGDLVSSQHAAYRNQGLYRLGN
FLFILLGVCCIYSLFNVISILIKQVLNWMLRKLSCRCCARCCPAPGAPLARRNAITPGSRLRRRLAALGA
DPAARDSDAEGRRLSGELISMRDLTASNKVSLALLQKQLSETANGYPRSVCVNTRQNGFSGGVGALGIMN
NRLAETSASR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022055
ORF Size 1290 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022055.1, NP_071338.1
RefSeq Size 1449 bp
RefSeq ORF 1293 bp
Locus ID 56660
UniProt ID Q9HB15
Cytogenetics 2p16.3
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
MW 46.7 kDa
Summary This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_022055" in other vectors (6)
SKU Description Size Price
RC208379L1 Lenti ORF clone of Human potassium channel, subfamily K, member 12 (KCNK12), Myc-DDK-tagged 10 ug
$757.00
RC208379L2 Lenti ORF clone of Human potassium channel, subfamily K, member 12 (KCNK12), mGFP tagged 10 ug
$757.00
RC208379L3 Lenti ORF clone of Human potassium channel, subfamily K, member 12 (KCNK12), Myc-DDK-tagged 10 ug
$757.00
RC208379L4 Lenti ORF clone of Human potassium channel, subfamily K, member 12 (KCNK12), mGFP tagged 10 ug
$757.00
RG208379 KCNK12 (tGFP-tagged) - Human potassium channel, subfamily K, member 12 (KCNK12) 10 ug
$489.00 $657.00
SC122152 KCNK12 (untagged)-Human potassium channel, subfamily K, member 12 (KCNK12) 10 ug
$457.00

Plasmid Map for RC208379

Download sequence for

Cloning scheme for RC208379

Circular map for RC208379

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