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RAB17 (NM_022449) Human Tagged ORF Clone

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SKU
RC208378
RAB17 (Myc-DDK-tagged)-Human RAB17, member RAS oncogene family (RAB17), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RAB17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208378 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACAGGCACACAGGACCCCCCAGCCCAGGGCTGCCCCCAGCCAGCCCCGTGTGTTCAAGCTGGTTC
TCCTGGGAAGTGGCTCCGTGGGTAAGTCCAGCTTGGCTCTTCGGTACGTGAAGAACGACTTCAAGAGTAT
CCTGCCTACGGTGGGCTGTGCGTTCTTCACAAAGGTGGTGGATGTGGGTGCCACCTCTCTGAAGCTTGAG
ATCTGGGACACAGCTGGCCAGGAGAAGTACCACAGCGTCTGCCACCTCTACTTCAGGGGTGCCAACGCTG
CGCTTCTGGTGTACGACATCACCAGGAAGGATTCCTTCCTCAAGGCTCAGCAGTGGCTGAAGGACCTGGA
GGAGGAGCTGCACCCAGGAGAAGTCCTGGTGATGCTGGTGGGCAACAAGACGGACCTCAGCCAGGAGCGG
GAGGTGACCTTCCAGGAAGGGAAGGAGTTTGCCGACAGCCAGAAGTTGCTGTTCATGGAAACTTCGGCCA
AACTGAACCACCAGGTGTCGGAGGTGTTCAATACAGTGGCCCAAGAGCTACTGCAGAGAAGCGACGAGGA
GGGCCAGGCTCTACGGGGGGATGCAGCTGTGGCTCTGAACAAGGGGCCCGCGAGGCAGGCCAAATGCTGC
GCCCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208378 protein sequence
Red=Cloning site Green=Tags(s)

MAQAHRTPQPRAAPSQPRVFKLVLLGSGSVGKSSLALRYVKNDFKSILPTVGCAFFTKVVDVGATSLKLE
IWDTAGQEKYHSVCHLYFRGANAALLVYDITRKDSFLKAQQWLKDLEEELHPGEVLVMLVGNKTDLSQER
EVTFQEGKEFADSQKLLFMETSAKLNHQVSEVFNTVAQELLQRSDEEGQALRGDAAVALNKGPARQAKCC
AH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022449
ORF Size 636 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022449.4
RefSeq Size 2018 bp
RefSeq ORF 639 bp
Locus ID 64284
UniProt ID Q9H0T7, A0A024R4A4
Cytogenetics 2q37.3
Domains RAB, RAN, ras, RAS, RHO
Protein Families Druggable Genome
MW 23.5 kDa
Summary The Rab subfamily of small GTPases plays an important role in the regulation of membrane trafficking. RAB17 is an epithelial cell-specific GTPase (Lutcke et al., 1993 PubMed 8486736).supplied by OMIM, Oct 2009
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"NM_022449" in other vectors (7)
SKU Description Size Price
RC208378L1 Lenti ORF clone of Human RAB17, member RAS oncogene family (RAB17), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC208378L2 Lenti ORF clone of Human RAB17, member RAS oncogene family (RAB17), transcript variant 1, mGFP tagged 10 ug
$600.00
RC208378L3 Lenti ORF clone of Human RAB17, member RAS oncogene family (RAB17), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC208378L4 Lenti ORF clone of Human RAB17, member RAS oncogene family (RAB17), transcript variant 1, mGFP tagged 10 ug
$600.00
RG208378 RAB17 (tGFP-tagged) - Human RAB17, member RAS oncogene family (RAB17), transcript variant 1 10 ug
$489.00 $500.00
SC112514 RAB17 (untagged)-Human RAB17, member RAS oncogene family (RAB17), transcript variant 1 10 ug
$300.00
SC324325 RAB17 (untagged)-Human RAB17, member RAS oncogene family (RAB17), transcript variant 1 10 ug
$300.00

Plasmid Map for RC208378

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208378
Circular map for RC208378

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