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FBXO25 (NM_183421) Human Tagged ORF Clone

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SKU
RC208287
FBXO25 (Myc-DDK-tagged)-Human F-box protein 25 (FBXO25), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXO25
Synonyms FBX25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208287 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATTTTTGGGTCAGGACTGGAGATCTCCTGGATGGAGTTGGATTAAGACAGAAGATGGCTGGAAGA
GATGTGAATCTTGTAGTCAGAAACTTGAAAGAGAGAATAACCATTGTAACATCAGTCACAGCATTATCTT
AAATAGTGAAGATGGAGAAATATTCAATAATGAAGAGCATGAATATGCATCGAAAAAAAGGAAAAAGGAC
CATTTTAGAAATGACACAAATACTCAAAGTTTTTATCGTGAAAAATGGATCTATGTCCATAAAGAAAGCA
CAAAGGAAAGGCATGGCTATTGCACCTTGGGAGAAGCCTTTAATCGGTTAGACTTCTCAAGTGCAATTCA
AGATATCCGAAGGTTCAATTATGTGGTCAAACTGTTGCAGCTAATTGCAAAATCCCAGTTAACTTCATTG
AGTGGCGTGGCACAGAAGAATTACTTCAACATTTTGGATAAAATCGTTCAAAAGGTTCTTGATGACCACC
ACAATCCTCGCTTAATCAAAGATCTTCTGCAAGACCTAAGCTCTACCCTCTGCATTCTTATTAGAGGAGT
AGGGAAGTCTGTATTAGTGGGAAACATCAATATTTGGATTTGCCGATTAGAAACTATTCTCGCCTGGCAA
CAACAGCTACAGGATCTTCAGATGACTAAGCAAGTGAACAATGGCCTCACCCTCAGTGACCTTCCTCTGC
ACATGCTGAACAACATCCTATACCGGTTCTCAGACGGATGGGACATCATCACCTTAGGCCAGGTGACCCC
CACGTTGTATATGCTTAGTGAAGACAGACAGCTGTGGAAGAAGCTTTGTCAGTACCATTTTGCTGAAAAG
CAGTTTTGTAGACATTTGATCCTTTCAGAAAAAGGTCATATTGAATGGAAGTTGATGTACTTTGCACTTC
AGAAACATTACCCAGCGAAGGAGCAGTACGGAGACACACTGCATTTCTGTCGGCACTGCAGCATTCTCTT
TTGGAAGGACTACCATCTTGCTTTACTATTCAAGGACTCAGGACACCCCTGCACGGCGGCCGACCCTGAC
AGCTGCTTCACGCCTGTGTCTCCGCAGCACTTCATCGACCTCTTCAAGTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208287 protein sequence
Red=Cloning site Green=Tags(s)

MPFLGQDWRSPGWSWIKTEDGWKRCESCSQKLERENNHCNISHSIILNSEDGEIFNNEEHEYASKKRKKD
HFRNDTNTQSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFNYVVKLLQLIAKSQLTSL
SGVAQKNYFNILDKIVQKVLDDHHNPRLIKDLLQDLSSTLCILIRGVGKSVLVGNINIWICRLETILAWQ
QQLQDLQMTKQVNNGLTLSDLPLHMLNNILYRFSDGWDIITLGQVTPTLYMLSEDRQLWKKLCQYHFAEK
QFCRHLILSEKGHIEWKLMYFALQKHYPAKEQYGDTLHFCRHCSILFWKDYHLALLFKDSGHPCTAADPD
SCFTPVSPQHFIDLFKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183421
ORF Size 1101 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183421.1, NP_904357.1
RefSeq Size 2529 bp
RefSeq ORF 1104 bp
Locus ID 26260
UniProt ID Q8TCJ0
Cytogenetics 8p23.3
MW 43.3 kDa
Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. provided by RefSeq, Jul 2008
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"NM_183421" in other vectors (6)
SKU Description Size Price
RC208287L1 Lenti ORF clone of Human F-box protein 25 (FBXO25), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC208287L2 Lenti ORF clone of Human F-box protein 25 (FBXO25), transcript variant 1, mGFP tagged 10 ug
$757.00
RC208287L3 Lenti ORF clone of Human F-box protein 25 (FBXO25), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC208287L4 Lenti ORF clone of Human F-box protein 25 (FBXO25), transcript variant 1, mGFP tagged 10 ug
$757.00
RG208287 FBXO25 (tGFP-tagged) - Human F-box protein 25 (FBXO25), transcript variant 1 10 ug
$489.00 $657.00
SC321837 FBXO25 (untagged)-Human F-box protein 25 (FBXO25), transcript variant 1 10 ug
$457.00

Plasmid Map for RC208287

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208287
Circular map for RC208287

file-alt Citations

FBXO25 promotes cell proliferation, invasion, and migration of NSCLC
Jiang, Zhang, Wang et al
Tumour Biol. (2016)
Cited by 15
AI Summary: OriGene products contributed to this study by providing the pCMV6-ddk-myc empty vector and the pCMV6-ddk-myc-FBXO25 (RC208287) plasmid, which were used to manipulate FBXO25 expression in cell experiments investigating its role in non-small-cell lung cancer.
Snippet: trypsin (Invitrogen). Plasmid transfection and small interfering RNA treatment The pCMV6-ddk-myc empty vector and pCMV6-ddk-myc- FBXO25 (RC208287) were purchased from Origene (Rockville, MD, USA). SiRNAs for FBXO25 ... Cite SaveSaving
Showing 1-1 of 1 papers.
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