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SPT3 (SUPT3H) (NM_181356) Human Tagged ORF Clone

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SKU
RC208269
SUPT3H (Myc-DDK-tagged)-Human suppressor of Ty 3 homolog (S. cerevisiae) (SUPT3H), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPT3
Synonyms SPT3; SPT3L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208269 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGTCTTTGGAACAACGTTCTTCAACTTTGACAGCTTCATAGGGAAAACAGATTCTATAGAAATAA
TTGGAAAATCAGTTTTTCCCTATTGTTATCACAATATCCTTCCACTGCTGAATGATTCTGGCAGGTATTC
TTTAGGTGATGCTAGAAGGCCTCTTCATGAAACAGCAGTTTTGGTAGAAGATGTGGTACACACTCAGTTA
ATTAATCTGTTACAGCAAGCTGCTGAAGTTTCTCAGCTGCGGGGAGCAAGGGTAATCACTCCTGAAGATC
TTCTGTTTTTGATGCGCAAAGATAAGAAAAAACTTAGAAGACTGCTAAAATACATGTTTATCCGAGACTA
CAAATCAAAGATTGTCAAAGGCATCGATGAGGATGATCTTCTCGAAGACAAATTGAGTGGCAGCAATAAT
GCGAACAAAAGACAAAAGATTGCTCAGGACTTCCTCAACTCTATTGACCAGACAGGAGAACTTTTAGCAA
TGTTTGAAGATGACGAAATTGATGAAGTTAAACAAGAAAGAATGGAGAGAGCAGAAAGACAAACTCGAAT
TATGGATTCAGCTCAATATGCAGAATTCTGTGAAAGTCGACAATTAAGTTTCTCCAAAAAAGCTTCCAAA
TTTCGAGACTGGTTGGACTGCAGCAGTATGGAGATAAAACCCAATGTTGTCGCAATGGAAATCTTAGCAT
ATTTAGCGTATGAAACTGTGGCACAGTTAGTGGATCTGGCTCTTCTTGTGAGGCAAGACATGGTAACCAA
GGCAGGGGACCCCTTCAGCCATGCCATTTCTGCAACCTTCATTCAGTATCACAACTCTGCTGAGAGCACT
GCAGCCTGTGGTGTTGAGGCTCACAGCGATGCCATCCAGCCCTGCCACATCAGAGAGGCCATTCGACGCT
ACAGCCACAGGATTGGCCCACTTTCCCCATTCACAAATGCCTACCGCAGGAATGGGATGGCTTTTCTAGC
CTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208269 protein sequence
Red=Cloning site Green=Tags(s)

MVVFGTTFFNFDSFIGKTDSIEIIGKSVFPYCYHNILPLLNDSGRYSLGDARRPLHETAVLVEDVVHTQL
INLLQQAAEVSQLRGARVITPEDLLFLMRKDKKKLRRLLKYMFIRDYKSKIVKGIDEDDLLEDKLSGSNN
ANKRQKIAQDFLNSIDQTGELLAMFEDDEIDEVKQERMERAERQTRIMDSAQYAEFCESRQLSFSKKASK
FRDWLDCSSMEIKPNVVAMEILAYLAYETVAQLVDLALLVRQDMVTKAGDPFSHAISATFIQYHNSAEST
AACGVEAHSDAIQPCHIREAIRRYSHRIGPLSPFTNAYRRNGMAFLAC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181356
ORF Size 984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181356.3
RefSeq Size 4509 bp
RefSeq ORF 987 bp
Locus ID 8464
UniProt ID O75486
Cytogenetics 6p21.1
Protein Families Stem cell - Pluripotency, Transcription Factors
MW 37.4 kDa
Summary Probable transcriptional activator.UniProtKB/Swiss-Prot Function
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"NM_181356" in other vectors (4)
SKU Description Size Price
RC208269L3 Lenti ORF clone of Human suppressor of Ty 3 homolog (S. cerevisiae) (SUPT3H), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC208269L4 Lenti ORF clone of Human suppressor of Ty 3 homolog (S. cerevisiae) (SUPT3H), transcript variant 2, mGFP tagged 10 ug
$600.00
RG208269 SUPT3H (tGFP-tagged) - Human suppressor of Ty 3 homolog (S. cerevisiae) (SUPT3H), transcript variant 2 10 ug
$489.00 $500.00
SC121014 SUPT3H (untagged)-Human suppressor of Ty 3 homolog (S. cerevisiae) (SUPT3H), transcript variant 2 10 ug
$300.00

Plasmid Map for RC208269

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208269
Circular map for RC208269

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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