DNAAF11 (NM_012472) Human Tagged ORF Clone

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SKU
RC208256
LRRC6 (Myc-DDK-tagged)-Human leucine rich repeat containing 6 (LRRC6)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DNAAF11
Synonyms CILD19; LRRC6; LRTP; tilB; TSLRP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208256 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTGGATCACAGAAGATCTTATTAGACGGAATGCTGAACACAACGACTGTGTCATTTTTTCCCTGG
AGGAACTCTCGTTGCATCAGCAAGAAATAGAAAGACTAGAACACATTGATAAATGGTGCCGGGATTTAAA
AATTCTCTATCTTCAAAATAATCTTATTGGGAAAATTGAAAATGTTAGCAAACTCAAGAAACTTGAATAT
TTGAATTTAGCTTTAAACAACATTGAAAAAATAGAAAACTTGGAAGGATGTGAAGAGCTGGCAAAACTTG
ACCTGACTGTGAATTTCATTGGAGAGCTGAGCAGCATTAAAAACTTGCAGCACAATATCCATCTGAAGGA
GCTCTTTCTCATGGGGAACCCATGTGCTTCCTTTGACCACTATAGGGAGTTCGTGGTAGCAACTCTTCCA
CAATTAAAGTGGTTGGATGGTAAAGAAATAGAGCCTTCAGAAAGGATTAAGGCATTGCAGGACTATTCAG
TAATTGAACCACAAATCAGAGAGCAGGAAAAAGATCACTGTCTTAAACGAGCCAAACTCAAGGAAGAGGC
TCAGAGGAAACACCAAGAAGAGGATAAAAATGAAGACAAGAGAAGTAACGCAGGCTTTGATGGACGTTGG
TACACAGACATCAATGCTACTCTTTCCTCTTTAGAGAGCAAAGACCACCTACAGGCACCAGACACAGAGG
AACACAACACAAAGAAATTAGACAACAGTGAAGATGACTTGGAATTCTGGAATAAGCCCTGTTTGTTTAC
TCCTGAATCAAGATTGGAAACTCTTAGACACATGGAAAAACAACGGAAGAAACAGGAAAAATTAAGTGAA
AAAAAGAAGAAAGTGAAACCACCCAGGACTTTGATCACTGAAGATGGGAAAGCCCTAAATGTGAATGAGC
CCAAAATTGACTTCTCTTTGAAAGATAACGAAAAGCAGATCATCCTGGACCTTGCTGTCTATAGGTATAT
GGATACCTCTTTAATCGATGTTGATGTGCAACCAACTTACGTGCGAGTAATGATCAAAGGAAAGCCATTT
CAGCTTGTCCTTCCTGCAGAAGTGAAACCCGATAGTAGTTCTGCTAAAAGATCTCAGACAACGGGTCATT
TGGTCATCTGCATGCCCAAGGTAGGAGAAGTAATCACAGGTGGTCAGCGAGCATTCAAATCTATGAAAAC
TACCTCGGACAGGAGCAGAGAACAAACAAATACAAGAAGCAAGCACATGGAGAAACTAGAAGTAGACCCT
AGCAAGCACTCATTCCCTGATGTGACTAACATAGTTCAAGAGAAAAAACACACACCCAGAAGACGACCTG
AACCCAAAATTATACCAAGTGAGGAAGACCCAACCTTTGAAGACAACCCTGAAGTGCCTCCGCTGATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208256 protein sequence
Red=Cloning site Green=Tags(s)

MGWITEDLIRRNAEHNDCVIFSLEELSLHQQEIERLEHIDKWCRDLKILYLQNNLIGKIENVSKLKKLEY
LNLALNNIEKIENLEGCEELAKLDLTVNFIGELSSIKNLQHNIHLKELFLMGNPCASFDHYREFVVATLP
QLKWLDGKEIEPSERIKALQDYSVIEPQIREQEKDHCLKRAKLKEEAQRKHQEEDKNEDKRSNAGFDGRW
YTDINATLSSLESKDHLQAPDTEEHNTKKLDNSEDDLEFWNKPCLFTPESRLETLRHMEKQRKKQEKLSE
KKKKVKPPRTLITEDGKALNVNEPKIDFSLKDNEKQIILDLAVYRYMDTSLIDVDVQPTYVRVMIKGKPF
QLVLPAEVKPDSSSAKRSQTTGHLVICMPKVGEVITGGQRAFKSMKTTSDRSREQTNTRSKHMEKLEVDP
SKHSFPDVTNIVQEKKHTPRRRPEPKIIPSEEDPTFEDNPEVPPLI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012472
ORF Size 1398 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012472.6
RefSeq Size 1888 bp
RefSeq ORF 1401 bp
Locus ID 23639
UniProt ID Q86X45
Cytogenetics 8q24.22
Domains LRR, LRRcap, LRR_SD22
MW 54.3 kDa
Summary The protein encoded by this gene contains several leucine-rich repeat domains and appears to be involved in the motility of cilia. Defects in this gene are a cause of primary ciliary dyskinesia-19 (CILD19). Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4, 11 and 22. provided by RefSeq, Apr 2016
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Documents
Resources
"NM_012472" in other vectors (4)
SKU Description Size Price
RC208256L3 Lenti ORF clone of Human leucine rich repeat containing 6 (LRRC6), Myc-DDK-tagged 10 ug
$757.00
RC208256L4 Lenti ORF clone of Human leucine rich repeat containing 6 (LRRC6), mGFP tagged 10 ug
$757.00
RG208256 LRRC6 (tGFP-tagged) - Human leucine rich repeat containing 6 (LRRC6) 10 ug
$489.00 $657.00
SC322640 LRRC6 (untagged)-Human leucine rich repeat containing 6 (LRRC6) 10 ug
$457.00

Plasmid Map for RC208256

Download sequence for

Cloning scheme for RC208256

Circular map for RC208256

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