KIF18A (NM_031217) Human Tagged ORF Clone
$822.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | KIF18A |
| Synonyms | MS-KIF18A; PPP1R99 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_031217 |
| ORF Size | 2694 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_031217.4 |
| RefSeq Size | 3463 bp |
| RefSeq ORF | 2697 bp |
| Locus ID | 81930 |
| UniProt ID | Q8NI77 |
| Cytogenetics | 11p14.1 |
| Domains | kinesin |
| Protein Families | Druggable Genome |
| MW | 102.3 kDa |
| Summary | KIF18A is a member of the kinesin superfamily of microtubule-associated molecular motors (see MIM 148760) that use hydrolysis of ATP to produce force and movement along microtubules (Luboshits and Benayahu, 2005 PubMed 15878648).supplied by OMIM, Aug 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_031217" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208235L1 | Lenti ORF clone of Human kinesin family member 18A (KIF18A), Myc-DDK-tagged | 10 ug |
$1,122.00
|
|
| RC208235L2 | Lenti ORF clone of Human kinesin family member 18A (KIF18A), mGFP tagged | 10 ug |
$1,122.00
|
|
| RC208235L3 | Lenti ORF clone of Human kinesin family member 18A (KIF18A), Myc-DDK-tagged | 10 ug |
$1,122.00
|
|
| RC208235L4 | Lenti ORF clone of Human kinesin family member 18A (KIF18A), mGFP tagged | 10 ug |
$1,122.00
|
|
| RG208235 | KIF18A (tGFP-tagged) - Human kinesin family member 18A (KIF18A) | 10 ug |
$789.00
$1,022.00
|
|
| SC108713 | KIF18A (untagged)-Human kinesin family member 18A (KIF18A) | 10 ug |
$823.00
|
|
| SC324239 | KIF18A (untagged)-Human kinesin family member 18A (KIF18A) | 10 ug |
$823.00
|
|
Citations
Chromosome alignment and Kif18A action rely on spindle-localized control of Cdk1 activity
Serpico, Pisauro, Trano et al
Front Cell Dev Biol (2024) 12, 1490781 DOI: 10.3389/fcell.2024.1490781
Serpico, Pisauro, Trano et al
Front Cell Dev Biol (2024) 12, 1490781 DOI: 10.3389/fcell.2024.1490781
Cited by 3
AI Summary: OriGene contributed to this study by providing the Myc-Flag-tagged human Kif18A-WT plasmid (Cat# RC208235), which was used to investigate the role of Kif18A in chromosome alignment during mitosis.
Snippet: 61840;Cat# 39872, respectively). 3XFlag-Wee1 expression construct was obtained as previously described (Visconti et al., 2015). Myc-Flag-tagged-human-Kif18A-WT (Kif18A-WT) plasmid was purchased from Origene (Cat# RC208235);Kif18A-AA mutant version was generated via mutagenesis: serine 674 was mutagenized into alanine (2020–2022 nt TCT→GCT) and serine 684 into alanine (2050–2052 nt TCT→G
Chromosome alignment relies on spindle-localized control of Cdk1 activity
Serpico, Pisauro, Trano et al
Research Square (2024)
Serpico, Pisauro, Trano et al
Research Square (2024)
Snippet: from Addgene (Cat# 61840;Cat# 39872, respectively). 3XFlag-Wee1 expression construct was obtained as previously described [16]. Myc-Flag-tagged-human-Kif18A-WT (Kif18A-WT) plasmid was purchased from Origene (Cat# RC208235);Kif18A-AA mutant version was generated via mutagenesis: serine 674 was mutagenized into alanine (2020–2022 nt TCT◊GCT) and serine 684 into alanine (2050–2052 nt TCT◊GC
Showing 1-2 of 2 papers.
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