P2Y8 (P2RY8) (NM_178129) Human Tagged ORF Clone

  • TrueORF®
SKU
RC208184
P2RY8 (Myc-DDK-tagged)-Human purinergic receptor P2Y, G-protein coupled, 8 (P2RY8)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol P2Y8
Synonyms P2Y8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208184 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCC
C

ATGCAGGTCCCGAACAGCACCGGCCCGGACAACGCGACGCTGCAGATGCTGCGGAACCCGGCGATCGCGG
TGGCCCTGCCCGTGGTGTACTCGCTGGTGGCGGCGGTCAGCATCCCGGGCAACCTCTTCTCTCTGTGGGT
GCTGTGCCGGCGCATGGGGCCCAGATCCCCGTCGGTCATCTTCATGATCAACCTGAGCGTCACGGACCTG
ATGCTGGCCAGCGTGTTGCCTTTCCAAATCTACTACCATTGCAACCGCCACCACTGGGTATTCGGGGTGC
TGCTTTGCAACGTGGTGACCGTGGCCTTTTACGCAAACATGTATTCCAGCATCCTCACCATGACCTGTAT
CAGCGTGGAGCGCTTCCTGGGGGTCCTGTACCCGCTCAGCTCCAAGCGCTGGCGCCGCCGTCGTTACGCG
GTGGCCGCGTGTGCAGGGACCTGGCTGCTGCTCCTGACCGCCCTGTCCCCGCTGGCGCGCACCGATCTCA
CCTACCCGGTGCACGCCCTGGGCATCATCACCTGCTTCGACGTCCTCAAGTGGACGATGCTCCCCAGCGT
GGCCATGTGGGCCGTGTTCCTCTTCACCATCTTCATCCTGCTGTTCCTCATCCCGTTCGTGATCACCGTG
GCTTGTTACACGGCCACCATCCTCAAGCTGTTGCGCACGGAGGAGGCGCACGGCCGGGAGCAGCGGAGGC
GCGCGGTGGGCCTGGCCGCGGTGGTCTTGCTGGCCTTTGTCACCTGCTTCGCCCCCAACAACTTCGTGCT
CCTGGCGCACATCGTGAGCCGCCTGTTCTACGGCAAGAGCTACTACCACGTGTACAAGCTCACGCTGTGT
CTCAGCTGCCTCAACAACTGTCTGGACCCGTTTGTTTATTACTTTGCGTCCCGGGAATTCCAGCTGCGCC
TGCGGGAATATTTGGGCTGCCGCCGGGTGCCCAGAGACACCCTGGACACGCGCCGCGAGAGCCTCTTCTC
CGCCAGGACCACGTCCGTGCGCTCCGAGGCCGGTGCGCACCCTGAAGGGATGGAGGGAGCCACCAGGCCC
GGCCTCCAGAGGCAGGAGAGTGTGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208184 protein sequence
Red=Cloning site Green=Tags(s)

MQVPNSTGPDNATLQMLRNPAIAVALPVVYSLVAAVSIPGNLFSLWVLCRRMGPRSPSVIFMINLSVTDL
MLASVLPFQIYYHCNRHHWVFGVLLCNVVTVAFYANMYSSILTMTCISVERFLGVLYPLSSKRWRRRRYA
VAACAGTWLLLLTALSPLARTDLTYPVHALGIITCFDVLKWTMLPSVAMWAVFLFTIFILLFLIPFVITV
ACYTATILKLLRTEEAHGREQRRRAVGLAAVVLLAFVTCFAPNNFVLLAHIVSRLFYGKSYYHVYKLTLC
LSCLNNCLDPFVYYFASREFQLRLREYLGCRRVPRDTLDTRRESLFSARTTSVRSEAGAHPEGMEGATRP
GLQRQESVF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178129
ORF Size 1077 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178129.5
RefSeq Size 4240 bp
RefSeq ORF 1080 bp
Locus ID 286530
UniProt ID Q86VZ1
Cytogenetics X;Y
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 40.6 kDa
Summary The protein encoded by this gene belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. This gene is moderately expressed in undifferentiated HL60 cells, and is located on both chromosomes X and Y. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_178129" in other vectors (4)
SKU Description Size Price
RC208184L3 Lenti ORF clone of Human purinergic receptor P2Y, G-protein coupled, 8 (P2RY8), Myc-DDK-tagged 10 ug
$757.00
RC208184L4 Lenti ORF clone of Human purinergic receptor P2Y, G-protein coupled, 8 (P2RY8), mGFP tagged 10 ug
$757.00
RG208184 P2RY8 (tGFP-tagged) - Human purinergic receptor P2Y, G-protein coupled, 8 (P2RY8) 10 ug
$657.00
SC107139 P2RY8 (untagged)-Human purinergic receptor P2Y, G-protein coupled, 8 (P2RY8) 10 ug
$457.00

Plasmid Map for RC208184

Download sequence for

Cloning scheme for RC208184

Circular map for RC208184

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