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TMTC1 (NM_175861) Human Tagged ORF Clone

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SKU
RC208155
TMTC1 (Myc-DDK-tagged)-Human transmembrane and tetratricopeptide repeat containing 1 (TMTC1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $709.00
In Stock*
Size
Bulk Requests & Clone Modifications

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Other products for "TMTC1 or NM_175861"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMTC1
Synonyms ARG99; OLF; TMTC1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208155 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCCATTCTACTTTCATGCAGTAAATATAATTTTACACTGCTTAGTGACTCTTGTGCTGATGTACA
CCTGTGATAAAACTGTCTTCAAGAATCGTGGACTTGCTTTTGTAACGGCATTGCTTTTTGCTGTACATCC
TATTCATACTGAGGCGGTGGCTGGGATCGTTGGCAGAGCGGACGTGTTAGCGTGTCTGCTGTTTCTATTG
GCCTTTCTCTCGTACAACAGGAGTCTGGATCAGGGCTGTGTTGGGGGAAGTTTCCCTTCCACGGTGTCTC
CCTTCTTCTTGCTGCTCAGTTTGTTTCTGGGGACCTGTGCGATGCTGGTGAAAGAGACAGGCATCACGGT
GTTTGGAGTGTGCTTGGTTTATGACCTCTTTTCCCTTTCCAACAAGCAAGACAAGTCGAGCAATGGGGCC
CTCTGTCCACGCAGCCCACAGCAGCCCGGGAGCCCCCAGCCCTCCTCACTGCCAGGCCATCCTCACCGGG
AGAATGGGAAGCAGCAGCGGTTCCCTCACAAAGGAGCTTGGGGTGGCTGCCACTCTCCACTGCCACCAGA
ACCCAAGAGCAGTGGATTCCCAGTGTCCCCACGAGCTGTGTGGTCCATGATGAGATTCCTCACCTATTCC
TACCTCTTGGCCTTCAATGTGTGGCTTCTGCTTGCACCCGTGACCCTGTGCTATGACTGGCAGGTCGGCA
GTATTCCTCTGGTAGAGACCATATGGGACATGCGGAACTTAGCCACCATCTTTCTGGCGGTTGTGATGGC
CTTATTGAGCCTGCACTGCTTAGCAGCCTTTAAGAGACTGGAGCACAAGGAGGTTTTAGTCGGCTTGTTG
TTCCTGGTGTTCCCGTTCATTCCAGCCAGCAACCTCTTCTTCAGGGTGGGTTTTGTGGTGGCGGAGAGAG
TCCTTTACATGCCTAGCATGGGCTACTGCATCCTTTTTGTGCACGGACTGAGCAAGCTCTGCACTTGGCT
GAATCGATGTGGGGCCACCACCCTGATTGTGTCCACTGTGTTGCTGCTGTTGCTTTTCTCTTGGAAAACT
GTGAAACAGAATGAAATTTGGCTGTCAAGAGAGTCCCTATTCAGGTCTGGAGTTCAAACTCTGCCCCACA
ATGCCAAGGTTCACTACAACTATGCCAATTTCCTGAAGGACCAAGGTCGGAACAAGGAAGCGATCTACCA
CTACAGAACAGCTCTCAAGTTGTATCCACGCCATGCAAGTGCGCTCAACAACCTTGGAACACTGACGAGA
GACACAGCAGAGGCAAAGATGTACTATCAGAGGGCTCTCCAGCTCCATCCACAGCATAACCGGGCTCTTT
TCAATCTGGGGAATCTCCTCAAGTCCCAGGAGAAAAAGGAAGAAGCTATCACCTTACTGAAGGATTCCAT
CAAATATGGTCCAGAGTTTGCAGATGCATATTCAAGCTTAGCTTCGTTATTGGCTGAGCAGGAGCGGTTT
AAAGAAGCTGAAGAAATATACCAAACTGGAATAAAGAACTGTCCAGACAGCTCAGATTTACACAACAACT
ATGGGGTTTTCTTAGTTGATACTGGCTTACCAGAAAAGGCAGTGGCCCATTACCAGCAGGCCATCAAACT
TAGCCCCAGTCATCACGTGGCCATGGTGAACTTGGGAAGACTCTACAGGTCACTGGGAGAGAACAGCATG
GCTGAAGAATGGTACAAGCGCGCCCTGCAGGTGGCACACAAAGCTGAGATATTGTCACCTTTGGGAGCAC
TGTATTACAACACTGGCCGATACGAAGAGGCTTTGCAGATTTACCAGGAAGCTGCAGCACTTCAGCCTTC
TCAGAGGGAGCTCCGCTTGGCACTGGCTCAGGTTTTGGCCGTGATGGGTCAGACAAAAGAAGCTGAAAAG
ATGACCAATCACATTGTGTCAGAGGAGACCGGATGCCTTGAATGCTATCGCCTCTTGTCAGCCATCTATA
GCAAGCAGGAGAACCACGACAAGGCACTTGATGCTATAGACAAGGCTCTCCAGCTGAAACCAAAGGACCC
AAAAGTCATTTCTGAACTTTTTTTCACAAAAGGAAACCAATTAAGAGAGCAGAACCTTCTCGACAAAGCT
TTTGAGAGCTATAGATTGGCTGTGCAACTAAACCCAGACCAAGCACAGGCCTGGATGAACATGGGTGGCA
TCCAACACATCAAGGGAAAATATGTGTCTGCAAGAGCTTATTATGAGAGAGCCTTACAGCTGGTTCCAGA
CAGCAAACTGCTGAAGGAAAATCTTGCCAAATTGGATCGCCTAGAAAAACGATTACAAGAAGTTCGAGAA
AAGGATCAAACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208155 protein sequence
Red=Cloning site Green=Tags(s)

MNPFYFHAVNIILHCLVTLVLMYTCDKTVFKNRGLAFVTALLFAVHPIHTEAVAGIVGRADVLACLLFLL
AFLSYNRSLDQGCVGGSFPSTVSPFFLLLSLFLGTCAMLVKETGITVFGVCLVYDLFSLSNKQDKSSNGA
LCPRSPQQPGSPQPSSLPGHPHRENGKQQRFPHKGAWGGCHSPLPPEPKSSGFPVSPRAVWSMMRFLTYS
YLLAFNVWLLLAPVTLCYDWQVGSIPLVETIWDMRNLATIFLAVVMALLSLHCLAAFKRLEHKEVLVGLL
FLVFPFIPASNLFFRVGFVVAERVLYMPSMGYCILFVHGLSKLCTWLNRCGATTLIVSTVLLLLLFSWKT
VKQNEIWLSRESLFRSGVQTLPHNAKVHYNYANFLKDQGRNKEAIYHYRTALKLYPRHASALNNLGTLTR
DTAEAKMYYQRALQLHPQHNRALFNLGNLLKSQEKKEEAITLLKDSIKYGPEFADAYSSLASLLAEQERF
KEAEEIYQTGIKNCPDSSDLHNNYGVFLVDTGLPEKAVAHYQQAIKLSPSHHVAMVNLGRLYRSLGENSM
AEEWYKRALQVAHKAEILSPLGALYYNTGRYEEALQIYQEAAALQPSQRELRLALAQVLAVMGQTKEAEK
MTNHIVSEETGCLECYRLLSAIYSKQENHDKALDAIDKALQLKPKDPKVISELFFTKGNQLREQNLLDKA
FESYRLAVQLNPDQAQAWMNMGGIQHIKGKYVSARAYYERALQLVPDSKLLKENLAKLDRLEKRLQEVRE
KDQT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175861
ORF Size 2322 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175861.1
RefSeq Size 8841 bp
RefSeq ORF 2325 bp
Locus ID 83857
UniProt ID Q8IUR5
Cytogenetics 12p11.22
Protein Families Transmembrane
MW 87.4 kDa
Summary Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. The 4 members of the TMTC family are O-mannosyl-transferases dedicated primarily to the cadherin superfamily, each member seems to have a distinct role in decorating the cadherin domains with O-linked mannose glycans at specific regions. Also acts as O-mannosyl-transferase on other proteins such as PDIA3.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175861" in other vectors (6)
SKU Description Size Price
RC208155L1 Lenti ORF clone of Human transmembrane and tetratricopeptide repeat containing 1 (TMTC1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,009.00
RC208155L2 Lenti ORF clone of Human transmembrane and tetratricopeptide repeat containing 1 (TMTC1), transcript variant 2, mGFP tagged 10 ug
$1,009.00
RC208155L3 Lenti ORF clone of Human transmembrane and tetratricopeptide repeat containing 1 (TMTC1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,009.00
RC208155L4 Lenti ORF clone of Human transmembrane and tetratricopeptide repeat containing 1 (TMTC1), transcript variant 2, mGFP tagged 10 ug
$1,009.00
RG208155 TMTC1 (tGFP-tagged) - Human transmembrane and tetratricopeptide repeat containing 1 (TMTC1), transcript variant 2 10 ug
$789.00 $909.00
SC128238 TMTC1 (untagged)-Human transmembrane and tetratricopeptide repeat containing 1 (TMTC1), transcript variant 2 10 ug
$710.00

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