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PANK4 (NM_018216) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC208116
PANK4 (Myc-DDK-tagged)-Human pantothenate kinase 4 (PANK4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $708.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "PANK4 or NM_018216"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PANK4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208116 representing NM_018216
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGTGTGGAGCGAGCGGCAGCGGGAGCAGCGGGGACAGTCTGGACAAGAGCATCACGCTGCCCC
CCGACGAGATCTTCCGCAACCTGGAGAACGCCAAGCGCTTCGCCATCGACATAGGCGGGTCGTTAACCAA
GCTGGCCTACTATTCAACGGTACAGCACAAAGTCGCCAAGGTGCGGTCTTTCGACCACTCCGGAAAGGAC
ACAGAACGTGAACATGAGCCGCCCTATGAGATTTCAGTTCAAGAAGAGATCACTGCTCGACTGCACTTCA
TTAAGTTTGAGAATACCTACATCGAAGCCTGCCTGGACTTCATCAAAGACCATCTCGTCAACACAGAGAC
CAAGGTCATCCAGGCGACCGGGGGCGGGGCCTACAAGTTCAAGGACCTCATCGAAGAGAAGCTGCGGCTG
AAAGTCGACAAGGAGGACGTGATGACGTGCCTGATTAAGGGGTGCAACTTCGTGCTCAAGAACATCCCCC
ATGAGGCCTTCGTGTACCAGAAGGATTCCGACCCTGAGTTCCGGTTCCAGACCAACCACCCCCACATTTT
CCCCTATCTTCTTGTCAATATCGGCTCTGGAGTCTCCATCGTGAAGGTGGAGACGGAGGACAGGTTCGAG
TGGGTCGGCGGCAGCTCCATTGGAGGCGGCACCTTCTGGGGGCTTGGCGCTCTGCTCACCAAAACGAAGA
AGTTTGACGAGCTCCTGCACCTGGCCTCGAGGGGCCAGCACAGCAATGTGGACATGCTGGTGCGGGACGT
CTACGGCGGCGCCCACCAGACTCTCGGGCTGAGCGGGAACCTCATCGCCAGCAGCTTCGGGAAGTCGGCC
ACCGCCGACCAAGAGTTCTCCAAAGAAGACATGGCGAAGAGCCTGCTGCACATGATCAGCAACGACATTG
GGCAGCTGGCCTGCCTCCACGCACGGCTGCACAGCCTGGACCGCGTGTACTTTGGAGGCTTCTTTATCCG
GGGCCACCCCGTGACCATGCGCACCATCACCTATAGCATCAACTTCTTCTCCAAGGGGGAAGTGCAGGCG
CTGTTTCTGAGGCACGAAGGCTACCTGGGAGCCATCGGAGCGTTCCTGAAAGGAGCTGAGCAGGACAATC
CTAACCAGTACAGCTGGGGAGAGAACTATGCAGGCAGCTCCGGGCTGATGAGTGCATCACCCGAGCTCGG
CCCGGCGCAGCGGGCGCGGAGTGGCACTTTTGACTTGCTGGAAATGGACCGGCTGGAGAGGCCACTGGTT
GACCTGCCGCTCCTCCTGGACCCGCCCTCCTACGTGCCCGACACGGTGGACCTCACCGATGACGCTCTGG
CCCGAAAATACTGGCTCACCTGCTTTGAGGAGGCCCTGGACGGGGTAGTGAAGCGCGCAGTGGCGAGCCA
GCCAGACTCTGTGGATGCAGCCGAGAGGGCGGAGAAGTTCCGGCAGAAGTACTGGAACAAGCTTCAGACC
CTGAGGCAGCAGCCCTTCGCCTATGGGACCCTGACCGTGCGCAGCCTGCTGGACACCAGGGAGCACTGTC
TGAACGAGTTCAACTTCCCGGATCCCTACTCCAAAGTGAAGCAGCGGGAGAATGGCGTGGCGCTGAGGTG
CTTCCCCGGGGTCGTGCGCTCCCTGGACGCGCTGGGCTGGGAGGAACGGCAGCTGGCGCTGGTGAAAGGC
CTCCTGGCGGGGAATGTCTTCGACTGGGGGGCCAAAGCCGTGTCTGCTGTCCTTGAATCCGACCCCTACT
TTGGGTTTGAAGAAGCAAAGAGGAAGTTACAAGAAAGACCCTGGCTCGTGGATTCCTACAGCGAGTGGCT
TCAGAGATTAAAGGGGCCCCCTCATAAATGTGCCTTAATTTTCGCAGATAACAGTGGAATAGACATCATT
TTGGGAGTCTTCCCCTTTGTCAGGGAGCTACTCCTTAGAGGGACAGAGGTCATCCTGGCGTGCAACTCAG
GCCCCGCCCTGAACGACGTGACCCACAGCGAGTCCCTCATCGTGGCAGAGCGTATTGCGGGCATGGACCC
TGTCGTGCACTCTGCGCTCCAGGAAGAGAGGCTGCTGCTGGTGCAGACGGGCTCCAGCTCCCCGTGCCTC
GACCTCAGCCGCCTGGATAAGGGGCTGGCCGCACTGGTGCGGGAGCGTGGCGCGGATCTGGTGGTCATCG
AGGGCATGGGCCGTGCTGTCCACACAAACTACCACGCAGCCCTGCGCTGCGAGAGCCTCAAGCTGGCCGT
CATCAAGAACGCGTGGCTGGCCGAGCGGCTGGGCGGCCGGCTCTTCAGCGTCATCTTCAAGTACGAGGTC
CCAGCCGAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208116 representing NM_018216
Red=Cloning site Green=Tags(s)

MAECGASGSGSSGDSLDKSITLPPDEIFRNLENAKRFAIDIGGSLTKLAYYSTVQHKVAKVRSFDHSGKD
TEREHEPPYEISVQEEITARLHFIKFENTYIEACLDFIKDHLVNTETKVIQATGGGAYKFKDLIEEKLRL
KVDKEDVMTCLIKGCNFVLKNIPHEAFVYQKDSDPEFRFQTNHPHIFPYLLVNIGSGVSIVKVETEDRFE
WVGGSSIGGGTFWGLGALLTKTKKFDELLHLASRGQHSNVDMLVRDVYGGAHQTLGLSGNLIASSFGKSA
TADQEFSKEDMAKSLLHMISNDIGQLACLHARLHSLDRVYFGGFFIRGHPVTMRTITYSINFFSKGEVQA
LFLRHEGYLGAIGAFLKGAEQDNPNQYSWGENYAGSSGLMSASPELGPAQRARSGTFDLLEMDRLERPLV
DLPLLLDPPSYVPDTVDLTDDALARKYWLTCFEEALDGVVKRAVASQPDSVDAAERAEKFRQKYWNKLQT
LRQQPFAYGTLTVRSLLDTREHCLNEFNFPDPYSKVKQRENGVALRCFPGVVRSLDALGWEERQLALVKG
LLAGNVFDWGAKAVSAVLESDPYFGFEEAKRKLQERPWLVDSYSEWLQRLKGPPHKCALIFADNSGIDII
LGVFPFVRELLLRGTEVILACNSGPALNDVTHSESLIVAERIAGMDPVVHSALQEERLLLVQTGSSSPCL
DLSRLDKGLAALVRERGADLVVIEGMGRAVHTNYHAALRCESLKLAVIKNAWLAERLGGRLFSVIFKYEV
PAE

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_018216
ORF Size 2319 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018216.1, NP_060686.1
RefSeq Size 2642 bp
RefSeq ORF 2322 bp
Locus ID 55229
UniProt ID Q9NVE7
Cytogenetics 1p36.32
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Pantothenate and CoA biosynthesis
MW 85.99 kDa
Summary This gene encodes a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is most abundant in muscle but is expressed in all tissues. provided by RefSeq, Jul 2008
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"NM_018216" in other vectors (4)
SKU Description Size Price
RC208116L3 Lenti-ORF clone of PANK4 (Myc-DDK-tagged)-Human pantothenate kinase 4 (PANK4) 10 ug
$1,008.00
RC208116L4 Lenti-ORF clone of PANK4 (mGFP-tagged)-Human pantothenate kinase 4 (PANK4) 10 ug
$1,008.00
RG208116 PANK4 (tGFP-tagged) - Human pantothenate kinase 4 (PANK4) 10 ug
$908.00
SC113651 PANK4 (untagged)-Human pantothenate kinase 4 (PANK4) 10 ug
$709.00

Plasmid Map for RC208116

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208116
Circular map for RC208116

file-alt Citations

LncRNA FEZF1-AS1 facilitates cisplatin resistance in non-small cell lung cancer through modulating the miR-32-5p-glutaminase axis
Lin, Wu, Liang et al
Am J Cancer Res (2024) 14 (6), 3153-3170 DOI: 10.62347/WUKN6549
AI Summary: OriGene contributed to this study by providing the LDHA overexpressing plasmid and control plasmid, which were used to investigate the molecular mechanisms underlying cisplatin resistance in non-small cell lung cancer.
Snippet: LDHA overexpressing plasmid and control plasmid were purchased from Origene.com. miR-32-5p precursor and control miRNA were purchased from GenePharma Inc. (
Kinome-Wide Synthetic Lethal Screen Identifies PANK4 as a Modulator of Temozolomide Resistance in Glioblastoma
Vella, Ditsiou, Chalari et al
Adv Sci (Weinh) (2024)
Cited by 10
AI Summary: OriGene products contributed to this study by providing the pCMV6-PANK4 overexpressing plasmid (#RC208116) and the pCMV6 empty vector (#PS100001), which were used to investigate the role of PANK4 in temozolomide resistance in glioblastoma.
Snippet: were purchased from Cell Signaling. The pCMV6-PANK4 overexpressing plasmid (#RC208116) and the pCMV6 empty vector (#PS100001) were purchased from Origene. pCMV6-PANK4 (D623A) and pCMV6-PANK4 (D659A) were generated and purchased from Genscript
Kinome-wide synthetic lethal screen identifies PANK4 as modulator of resistance in glioblastoma
Vella, Ditsiou, Chalari et al
Research Square (2023)
Cited by 1
Snippet: The pCMV6-PANK4 overexpressing plasmid (#RC208116) and the pCMV6 empty vector (#PS100001) were purchased from Origene. The pLKO.1-puro PANK4 shRNA (#SH0111;targeting sequence: GGACTCTTCTGCTTGTCACTT) and the pLKO.1-puro non-targeting scrambled shRNA (#SHC016) were purchased from Sigma-Aldrich
Showing 1-3 of 3 papers.
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