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ARPC1A (NM_006409) Human Tagged ORF Clone

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SKU
RC208053
ARPC1A (Myc-DDK-tagged)-Human actin related protein 2/3 complex, subunit 1A, 41kDa (ARPC1A), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ARPC1A or NM_006409"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ARPC1A
Synonyms Arc40; HEL-68; HEL-S-307; SOP2Hs; SOP2L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208053 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCACTGCATCAGTTTTTACTAGAGCCAATCACCTGTCATGCCTGGAACAGGGATCGTACTCAGATTG
CCCTCAGTCCCAATAATCACGAAGTGCACATCTATAAGAAGAACGGGAGCCAGTGGGTGAAAGCTCATGA
ACTCAAGGAGCACAACGGACACATCACAGGTATTGACTGGGCTCCCAAGAGCGACCGCATTGTCACTTGT
GGGGCAGACCGCAATGCCTATGTCTGGAGTCAGAAAGATGGTGTTTGGAAGCCAACCCTGGTGATCCTGA
GAATTAATCGCGCAGCTACTTTTGTGAAGTGGTCCCCCCTAGAGAACAAATTTGCTGTGGGAAGTGGAGC
ACGACTCATTTCTGTTTGTTACTTTGAGTCTGAAAATGACTGGTGGGTGAGCAAGCACATTAAAAAGCCG
ATTCGCTCCACAGTCCTCAGCTTGGATTGGCATCCCAACAACGTTTTGCTGGCAGCAGGATCATGTGACT
TCAAATGCAGAGTGTTTTCTGCCTACATTAAAGAAGTGGATGAAAAGCCAGCCAGCACGCCCTGGGGCAG
CAAGATGCCTTTTGGGCAGCTGATGTCAGAGTTTGGTGGCAGTGGCACTGGTGGCTGGGTCCACGGGGTA
AGCTTCTCTGCCAGTGGGAGCCGCCTGGCCTGGGTCAGCCACGACAGCACCGTGTCTGTTGCTGATGCCT
CAAAAAGTGTGCAGGTCTCGACTCTGAAGACAGAGTTCCTGCCGCTCCTAAGTGTGTCATTTGTCTCAGA
GAACAGCGTCGTGGCTGCTGGCCATGACTGCTGCCCAATGCTCTTTAACTACGATGACCGCGGCTGCCTG
ACCTTCGTCTCCAAGTTAGATATTCCAAAACAGAGCATCCAACGCAACATGTCTGCCATGGAACGCTTCC
GCAACATGGACAAGAGAGCCACAACTGAGGACCGCAACACGGCCTTGGAGACGCTGCACCAGAATAGCAT
CACTCAAGTCTCTATTTATGAGGTGGACAAGCAAGATTGTCGCAAATTTTGCACTACTGGCATCGATGGA
GCCATGACAATTTGGGATTTCAAGACCCTCGAGTCTTCCATCCAGGGCCTCCGGATAATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208053 protein sequence
Red=Cloning site Green=Tags(s)

MSLHQFLLEPITCHAWNRDRTQIALSPNNHEVHIYKKNGSQWVKAHELKEHNGHITGIDWAPKSDRIVTC
GADRNAYVWSQKDGVWKPTLVILRINRAATFVKWSPLENKFAVGSGARLISVCYFESENDWWVSKHIKKP
IRSTVLSLDWHPNNVLLAAGSCDFKCRVFSAYIKEVDEKPASTPWGSKMPFGQLMSEFGGSGTGGWVHGV
SFSASGSRLAWVSHDSTVSVADASKSVQVSTLKTEFLPLLSVSFVSENSVVAAGHDCCPMLFNYDDRGCL
TFVSKLDIPKQSIQRNMSAMERFRNMDKRATTEDRNTALETLHQNSITQVSIYEVDKQDCRKFCTTGIDG
AMTIWDFKTLESSIQGLRIM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006409
ORF Size 1110 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006409.4
RefSeq Size 1623 bp
RefSeq ORF 1113 bp
Locus ID 10552
UniProt ID Q92747, V9HVZ6
Cytogenetics 7q22.1
Domains WD40
Protein Pathways Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
MW 41.6 kDa
Summary This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2010
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"NM_006409" in other vectors (5)
SKU Description Size Price
RC208053L3 Lenti ORF clone of Human actin related protein 2/3 complex, subunit 1A, 41kDa (ARPC1A), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC208053L4 Lenti ORF clone of Human actin related protein 2/3 complex, subunit 1A, 41kDa (ARPC1A), transcript variant 1, mGFP tagged 10 ug
$757.00
RG208053 ARPC1A (tGFP-tagged) - Human actin related protein 2/3 complex, subunit 1A, 41kDa (ARPC1A), transcript variant 1 10 ug
$489.00 $657.00
SC116099 ARPC1A (untagged)-Human actin related protein 2/3 complex, subunit 1A, 41kDa (ARPC1A), transcript variant 1 10 ug
$457.00
SC324215 ARPC1A (untagged)-Human actin related protein 2/3 complex, subunit 1A, 41kDa (ARPC1A), transcript variant 1 10 ug
$457.00

Plasmid Map for RC208053

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208053
Circular map for RC208053

file-alt Citations

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