DDX26 (INTS6) (NM_012141) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC208036L4
Lenti ORF clone of Human integrator complex subunit 6 (INTS6), transcript variant 1, mGFP tagged
  $1,112.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DDX26
Synonyms DBI-1; DDX26; DDX26A; DICE1; HDB; INT6; Notchl2
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC208036).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012141
ORF Size 2661 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012141.2
RefSeq Size 7465 bp
RefSeq ORF 2664 bp
Locus ID 26512
UniProt ID Q9UL03
Cytogenetics 13q14.3
Protein Families Druggable Genome
MW 100.4 kDa
Summary DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. The protein encoded by this gene is a DEAD box protein that is part of a complex that interacts with the C-terminus of RNA polymerase II and is involved in 3' end processing of snRNAs. In addition, this gene is a candidate tumor suppressor and is located in the critical region of loss of heterozygosity (LOH). Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2015
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Documents
Resources
"NM_012141" in other vectors (5)
SKU Description Size Price
RC208036 INTS6 (Myc-DDK-tagged)-Human integrator complex subunit 6 (INTS6), transcript variant 1 10 ug
$812.00
RC208036L3 Lenti ORF clone of Human integrator complex subunit 6 (INTS6), transcript variant 1, Myc-DDK-tagged 10 ug
$1,112.00
RG208036 INTS6 (tGFP-tagged) - Human integrator complex subunit 6 (INTS6), transcript variant 1 10 ug
$1,012.00
SC115535 INTS6 (untagged)-Human integrator complex subunit 6 (INTS6), transcript variant 1 10 ug
$813.00
SC322618 INTS6 (untagged)-Human integrator complex subunit 6 (INTS6), transcript variant 1 10 ug
$813.00

Plasmid Map for RC208036L4

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Cloning scheme for RC208036L4

Circular map for RC208036L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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