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EXOC5 (NM_006544) Human Tagged ORF Clone

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SKU
RC207906
EXOC5 (Myc-DDK-tagged)-Human exocyst complex component 5 (EXOC5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $648.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EXOC5
Synonyms HSEC10; PRO1912; SEC10; SEC10L1; SEC10P
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207906 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACCACGGCCGAGCTCTTCGAGGAGCCTTTTGTGGCAGATGAATATATTGAACGTCTTGTATGGA
GAACCCCAGGAGGAGGCTCTAGAGGTGGACCTGAAGCTTTTGATCCTAAAAGATTATTAGAAGAATTTGT
AAATCATATTCAGGAACTCCAGATAATGGATGAAAGGATTCAGAGGAAAGTAGAGAAACTAGAGCAACAA
TGTCAGAAAGAAGCCAAGGAATTTGCCAAGAAGGTACAAGAGCTGCAGAAAAGCAATCAGGTTGCCTTCC
AACATTTCCAAGAACTAGATGAGCACATTAGCTATGTAGCAACTAAAGTCTGTCACCTTGGAGACCAGTT
AGAGGGGGTAAACACACCCAGACAACGGGCAGTGGAGGCTCAGAAATTGATGAAATACTTTAATGAGTTT
CTAGATGGAGAATTGAAATCTGATGTTTTTACAAATTCTGAAAAGATAAAGGAAGCAGCAGACATCATTC
AGAAGTTGCACCTAATTGCCCAAGAGTTACCTTTTGATAGATTTTCAGAAGTTAAATCCAAAATTGCAAG
TAAATACCATGATTTAGAATGCCAGCTGATTCAGGAGTTTACCAGTGCTCAAAGAAGAGGTGAAATCTCC
AGAATGAGAGAAGTAGCAGCAGTTTTACTTCATTTTAAGGGTTATTCCCATTGTGTTGATGTTTATATAA
AGCAGTGCCAGGAGGGTGCTTATTTGAGAAATGATATATTTGAAGACGCTGGAATACTCTGTCAAAGAGT
GAACAAACAAGTTGGAGATATCTTCAGTAATCCAGAAACAGTCCTGGCTAAACTTATTCAAAATGTATTT
GAAATCAAACTACAGAGTTTTGTGAAAGAGCAGTTAGAAGAATGTAGGAAGTCCGATGCAGAGCAATATC
TCAAAAATCTCTATGATCTGTATACAAGAACCACCAATCTTTCCAGCAAGCTGATGGAGTTTAATTTAGG
TACTGATAAACAGACTTTCTTGTCTAAGCTTATCAAATCCATTTTCATTTCCTATTTGGAGAACTATATT
GAGGTGGAGACTGGATATTTGAAAAGCAGAAGTGCTATGATCCTACAGCGCTATTATGATTCGAAAAACC
ATCAAAAGAGATCCATTGGCACAGGAGGTATTCAAGATTTGAAGGAAAGAATTAGACAGCGTACCAACTT
ACCACTTGGGCCAAGTATCGATACTCATGGGGAGACTTTTCTATCCCAAGAAGTGGTGGTTAATCTTTTA
CAAGAAACCAAACAAGCCTTTGAAAGATGTCATAGGCTCTCTGATCCTTCTGACTTACCAAGGAATGCCT
TCAGAATTTTTACCATTCTTGTGGAATTTTTATGTATTGAGCATATTGATTATGCTTTGGAAACAGGACT
TGCTGGAATTCCCTCTTCAGATTCTAGGAATGCAAATCTTTATTTTTTGGACGTTGTGCAACAGGCCAAT
ACTATTTTTCATCTTTTTGACAAACAGTTTAATGATCACCTTATGCCACTAATAAGCTCTTCTCCTAAGT
TATCTGAATGCCTTCAGAAGAAAAAAGAAATAATTGAACAAATGGAGATGAAATTGGATACTGGCATTGA
TAGGACATTAAATTGTATGATTAGACAGATGAAGCATATTTTGGCTGCAGAACAGAAGAAAACAGATTTT
AAGCCAGAAGATGAAAACAATGTTTTGATTCAATATACTAATGCCTGTGTAAAAGTCTGTGCTTACGTAA
GAAAACAAGTGGAGAAGATTAAAAATTCCATGGATGGGAAGAATGTGGATACAGTTTTGATGGAACTTGG
AGTACGTTTTCATCGACTTATCTATGAGCATCTTCAACAATATTCCTACAGTTGTATGGGTGGCATGTTG
GCCATTTGTGATGTAGCCGAATATAGGAAGTGTGCCAAAGACTTCAAGATTCCAATGGTATTACATCTTT
TTGATACTCTGCATGCTCTTTGCAATCTTCTGGTAGTTGCCCCAGATAATTTAAAGCAAGTCTGCTCAGG
AGAACAACTTGCTAATCTGGACAAGAATATACTTCACTCCTTCGTACAACTTCGTGCTGATTATAGATCT
GCCCGCCTTGCTCGACACTTCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207906 protein sequence
Red=Cloning site Green=Tags(s)

MATTAELFEEPFVADEYIERLVWRTPGGGSRGGPEAFDPKRLLEEFVNHIQELQIMDERIQRKVEKLEQQ
CQKEAKEFAKKVQELQKSNQVAFQHFQELDEHISYVATKVCHLGDQLEGVNTPRQRAVEAQKLMKYFNEF
LDGELKSDVFTNSEKIKEAADIIQKLHLIAQELPFDRFSEVKSKIASKYHDLECQLIQEFTSAQRRGEIS
RMREVAAVLLHFKGYSHCVDVYIKQCQEGAYLRNDIFEDAGILCQRVNKQVGDIFSNPETVLAKLIQNVF
EIKLQSFVKEQLEECRKSDAEQYLKNLYDLYTRTTNLSSKLMEFNLGTDKQTFLSKLIKSIFISYLENYI
EVETGYLKSRSAMILQRYYDSKNHQKRSIGTGGIQDLKERIRQRTNLPLGPSIDTHGETFLSQEVVVNLL
QETKQAFERCHRLSDPSDLPRNAFRIFTILVEFLCIEHIDYALETGLAGIPSSDSRNANLYFLDVVQQAN
TIFHLFDKQFNDHLMPLISSSPKLSECLQKKKEIIEQMEMKLDTGIDRTLNCMIRQMKHILAAEQKKTDF
KPEDENNVLIQYTNACVKVCAYVRKQVEKIKNSMDGKNVDTVLMELGVRFHRLIYEHLQQYSYSCMGGML
AICDVAEYRKCAKDFKIPMVLHLFDTLHALCNLLVVAPDNLKQVCSGEQLANLDKNILHSFVQLRADYRS
ARLARHFS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006544
ORF Size 2124 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006544.4
RefSeq Size 8522 bp
RefSeq ORF 2127 bp
Locus ID 10640
UniProt ID O00471
Cytogenetics 14q22.3
MW 82 kDa
Summary The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. provided by RefSeq, Jul 2008
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"NM_006544" in other vectors (4)
SKU Description Size Price
RC207906L3 Lenti ORF clone of Human exocyst complex component 5 (EXOC5), Myc-DDK-tagged 10 ug
$948.00
RC207906L4 Lenti ORF clone of Human exocyst complex component 5 (EXOC5), mGFP tagged 10 ug
$948.00
RG207906 EXOC5 (tGFP-tagged) - Human exocyst complex component 5 (EXOC5) 10 ug
$789.00 $848.00
SC127665 EXOC5 (untagged)-Human exocyst complex component 5 (EXOC5) 10 ug
$649.00

Plasmid Map for RC207906

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207906
Circular map for RC207906

file-alt Citations

SmartBac, a new baculovirus system for large protein complex production
Zhai, Zhang, Yu et al
Journal of Structural Biology: X (2019)
Snippet: All human exocyst genes were purchased from Origene, USA. For each gene, two cycles of PCR were done to produce the target overlapped ends (M0492, NEB). 4V2G was digested by NdeI, KpnI and EcoRI (NEB)
Showing 1-1 of 1 papers.
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