CCDC6 (NM_005436) Human Tagged ORF Clone

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SKU
RC207773
CCDC6 (Myc-DDK-tagged)-Human coiled-coil domain containing 6 (CCDC6)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC6
Synonyms D10S170; H4; PTC; TPC; TST1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207773 representing NM_005436
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACAGCGCCAGCGAGAGCGACACGGACGGGGCGGGGGGCAACAGCAGCAGCTCGGCCGCCATGC
AGTCGTCCTGCTCGTCGACCTCGGGCGGCGGCGGTGGCGGCGGGGGAGGCGGCGGCGGTGGGAAGTCGGG
GGGCATTGTCATCTCGCCGTTCCGCCTGGAGGAGCTCACCAACCGCCTGGCCTCGCTGCAGCAAGAGAAC
AAGGTGCTGAAGATAGAGCTGGAGACCTACAAACTGAAGTGCAAGGCACTGCAGGAGGAGAACCGCGACC
TGCGCAAAGCCAGCGTGACCATCCAAGCCAGGGCTGAGCAGGAAGAAGAATTCATTAGTAACACTTTATT
CAAGAAAATTCAGGCTTTGCAGAAGGAGAAAGAAACCCTTGCTGTAAATTATGAGAAAGAAGAAGAATTC
CTCACTAATGAGCTCTCCAGAAAATTGATGCAGTTGCAGCATGAGAAAGCCGAACTAGAACAGCATCTTG
AACAAGAGCAGGAATTTCAGGTCAACAAACTGATGAAGAAAATTAAAAAACTGGAGAATGACACCATTTC
TAAGCAACTTACATTAGAACAGTTGAGACGGGAGAAGATTGACCTTGAAAATACATTGGAACAAGAACAA
GAAGCACTAGTTAATCGCCTCTGGAAAAGGATGGATAAGCTTGAAGCTGAAAAGCGAATCCTGCAGGAAA
AATTAGACCAGCCCGTCTCTGCTCCACCATCGCCTAGAGATATCTCCATGGAGATTGATTCTCCAGAAAA
TATGATGCGTCACATCAGGTTTTTAAAGAATGAAGTGGAACGGCTGAAGAAGCAACTGAGAGCTGCTCAG
TTACAGCATTCAGAGAAAATGGCACAGTATCTGGAGGAGGAACGTCACATGAGAGAAGAGAACTTGAGGC
TCCAGAGGAAGCTGCAGAGGGAGATGGAGAGAAGAGAAGCCCTCTGTCGACAGCTCTCCGAGAGTGAGTC
CAGCTTAGAAATGGACGACGAAAGGTATTTTAATGAGATGTCTGCACAAGGATTAAGACCTCGCACTGTG
TCCAGCCCGATCCCTTACACACCTTCTCCGAGTTCAAGCAGGCCTATATCACCTGGTCTATCATATGCAA
GTCACACGGTTGGTTTCACGCCACCAACTTCACTGACTAGAGCTGGAATGTCTTATTACAATTCCCCGGG
TCTTCACGTGCAGCACATGGGAACATCCCATGGTATCACAAGGCCTTCACCACGGAGAAGCAACAGTCCT
GACAAATTCAAACGGCCCACGCCGCCTCCATCTCCCAACACACAGACCCCAGTCCAGCCACCTCCGCCTC
CACCTCCGCCACCCATGCAGCCCACGGTCCCCTCAGCAGCCACCTCGCAGCCTACTCCTTCGCAACATTC
GGCGCACCCCTCCTCCCAGCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207773 representing NM_005436
Red=Cloning site Green=Tags(s)

MADSASESDTDGAGGNSSSSAAMQSSCSSTSGGGGGGGGGGGGGKSGGIVISPFRLEELTNRLASLQQEN
KVLKIELETYKLKCKALQEENRDLRKASVTIQARAEQEEEFISNTLFKKIQALQKEKETLAVNYEKEEEF
LTNELSRKLMQLQHEKAELEQHLEQEQEFQVNKLMKKIKKLENDTISKQLTLEQLRREKIDLENTLEQEQ
EALVNRLWKRMDKLEAEKRILQEKLDQPVSAPPSPRDISMEIDSPENMMRHIRFLKNEVERLKKQLRAAQ
LQHSEKMAQYLEEERHMREENLRLQRKLQREMERREALCRQLSESESSLEMDDERYFNEMSAQGLRPRTV
SSPIPYTPSPSSSRPISPGLSYASHTVGFTPPTSLTRAGMSYYNSPGLHVQHMGTSHGITRPSPRRSNSP
DKFKRPTPPPSPNTQTPVQPPPPPPPPPMQPTVPSAATSQPTPSQHSAHPSSQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005436
ORF Size 1422 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005436.5
RefSeq Size 5842 bp
RefSeq ORF 1425 bp
Locus ID 8030
UniProt ID Q16204, Q05CP8
Cytogenetics 10q21.2
Protein Pathways Pathways in cancer, Thyroid cancer
MW 53.3 kDa
Summary This gene encodes a coiled-coil domain-containing protein. The encoded protein is ubiquitously expressed and may function as a tumor suppressor. A chromosomal rearrangement resulting in the expression of a fusion gene containing a portion of this gene and the intracellular kinase-encoding domain of the ret proto-oncogene is the cause of thyroid papillary carcinoma.provided by RefSeq, Sep 2010
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Documents
Resources
"NM_005436" in other vectors (6)
SKU Description Size Price
RC207773L1 Lenti-ORF clone of CCDC6 (Myc-DDK-tagged)-Human coiled-coil domain containing 6 (CCDC6) 10 ug
$986.00
RC207773L2 Lenti-ORF clone of CCDC6 (mGFP-tagged)-Human coiled-coil domain containing 6 (CCDC6) 10 ug
$986.00
RC207773L3 Lenti-ORF clone of CCDC6 (Myc-DDK-tagged)-Human coiled-coil domain containing 6 (CCDC6) 10 ug
$986.00
RC207773L4 Lenti-ORF clone of CCDC6 (mGFP-tagged)-Human coiled-coil domain containing 6 (CCDC6) 10 ug
$986.00
RG207773 CCDC6 (tGFP-tagged) - Human coiled-coil domain containing 6 (CCDC6) 10 ug
$886.00
SC116736 CCDC6 (untagged)-Human coiled-coil domain containing 6 (CCDC6) 10 ug
$686.00

Plasmid Map for RC207773

Download sequence for

Cloning scheme for RC207773

Circular map for RC207773

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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