NSE2 (NSMCE2) (NM_173685) Human Tagged ORF Clone
SKU
RC207639
NSMCE2 (Myc-DDK-tagged)-Human non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2)
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NSE2 |
| Synonyms | C8orf36; MMS21; NSE2; ZMIZ7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_173685 |
| ORF Size | 741 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_173685.4 |
| RefSeq Size | 1225 bp |
| RefSeq ORF | 744 bp |
| Locus ID | 286053 |
| UniProt ID | Q96MF7 |
| Cytogenetics | 8q24.13 |
| MW | 27.9 kDa |
| Summary | This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. A knockout of the orthologous mouse gene is lethal prior to embryonic day 10.5. Naturally occurring mutations in this gene, that abolish the SUMO ligase activity, are associated with primordial dwarfism and extreme insulin resistance. provided by RefSeq, Mar 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_173685" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207639L1 | Lenti ORF clone of Human non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC207639L2 | Lenti ORF clone of Human non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2), mGFP tagged | 10 ug |
$750.00
|
|
| RC207639L3 | Lenti ORF clone of Human non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC207639L4 | Lenti ORF clone of Human non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2), mGFP tagged | 10 ug |
$750.00
|
|
| RG207639 | NSMCE2 (tGFP-tagged) - Human non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2) | 10 ug |
$650.00
|
|
| SC123332 | NSMCE2 (untagged)-Human non-SMC element 2, MMS21 homolog (S. cerevisiae) (NSMCE2) | 10 ug |
$300.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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