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ZNF443 (NM_005815) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC207567
ZNF443 (Myc-DDK-tagged)-Human zinc finger protein 443 (ZNF443). Note: ORF is codon optimized
ORF Plasmid:
DDK tGFP
AAV Particle:
DDK
$589.00 $614.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF443
Synonyms ZK1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207567 ORF sequence, codon optimized.
Due to the complexity of NM_005815, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCCGTCGCACTCGAGGATGTTGCCGTTAATTTTACCCGCGAAGAATGGGCTCTGCTGGGACCCT
GCCAGAAAAACCTGTACAAGGACGTTATGCAGGAAACGATCCGGAATCTGGACTGTGTCGTCATGAAGTG
GAAAGATCAGAACATTGAAGATCAGTACCGGTATCCACGGAAGAATCTCAGATGCAGAATGCTGGAGCGC
TTCGTTGAGTCCAAGGACGGGACCCAGTGCGGCGAAACCAGTTCCCAGATACAAGACTCCATCGTCACTA
AAAACACCCTGCCCGGGGTTGGCCCTTGCGAATCATCCATGCGGGGCGAGAAAGTAATGGGCCATAGCTC
ATTGAACTGCTATATACGGGTGGGCGCGGGCCATAAGCCCCACGAGTATCATGAGTGTGGAGAAAAACCA
GACACGCACAAGCAGCGCGGCAAAGCTTTTTCCTATCATAATAGCTTTCAGACTCACGAACGGCTGCACA
CCGGGAAGAAACCGTATGATTGCAAGGAATGCGGGAAATCATTCAGCTCCCTGGGAAACCTCCAGCGGCA
CATGGCCGTCCAACGGGGAGATGGGCCATATAAATGCAAACTGTGCGGCAAAGCATTTTTCTGGCCATCC
CTTCTGCATATGCACGAGAGAACCCACACAGGCGAAAAACCTTACGAGTGCAAGCAATGCAGCAAGGCCT
TCTCATTTTACAGTAGCTATCTTCGCCACGAGCGGACCCATACCGGCGAAAAACCTTATGAATGCAAGCA
GTGCAGCAAAGCCTTTCCGTTTTACAGCAGCTACCTGCGCCACGAACGCACTCATACCGGCGAGAAGCCG
TACAAATGCAAACAATGCAGCAAGGCCTTTCCTGATTCAAGCTCTTGTCTTATTCACGAGCGAACTCACA
CAGGGGAAAAACCTTACACCTGCAAACAGTGCGGCAAGGCGTTCTCTGTGTCTGGGTCACTCCAAAGGCA
CGAAACTACACACTCCGCTGAAAAGCCATACGCCTGTCAACAGTGTGGGAAAGCCTTTCACCATCTGGGT
AGCTTTCAGCGCCATATGATCAGGCACACGGGCAATGGACCGCATAAGTGCAAGATTTGCGGCAAAGGCT
TTGATTGTCCTTCTAGTCTCCAGTCCCACGAGAGAACCCATACAGGGGAGAAGCCCTATGAGTGCAAACA
ATGCGGTAAGGCTCTCTCCCACCGGAGCTCTTTTCGCAGCCACATGATAATGCATACTGGTGACGGGCCC
CACAAATGCAAGGTTTGTGGTAAAGCGTTCGTCTATCCTTCTGTGTTCCAGCGGCATGAGAGAACACACA
CCGCCGAGAAGCCCTACAAATGCAAACAGTGTGGCAAGGCTTACCGCATTAGCTCCAGCCTGAGGAGGCA
CGAGACAACTCACACAGGGGAAAAGCCTTACAAGTGCAAGCTGGGCAAGGCCTTCATCGATTTCTGCTCA
TTTCAGAACCACAAGACGACTCACACTGGCGAAAAGCCATATGAATGCAAGGAGTGCGGCAAGGCCTTTA
GCCGATTCCGATACCTCTCACGACACAAAAGGACCCACACAGGGGAGAAACCCTACGAGTGCAAAACATG
TCGGAAAGCCTTTGGACACTACGACAACCTGAAGGTTCATGAAAGAATACATAGTGGTGAGAAACCCTAT
GAGTGCAAGGAGTGCGGAAAAGCTTTCTCTTGGCTGACCTGTTTCCTCAGACACGAACGCATTCATATGA
GAGAAAAGTCTTATGAGTGCCCGCAGTGTGGCAAGGCGTTTACACATTCAAGATTTTTGCAAGGACATGA
GAAAACCCATACGGGAGAAAACCCATATGAGTGTAAAGAATGTGGAAAGGCGTTCGCCTCCCTGAGTAGT
CTGCACCGACACAAGAAGACACACTGGAAAAAGACGCACACCGGGGAGAATCCTTACGAATGTAAAGAGT
GCGGCAAAGCTTTTGCCTCACTCAGCAGCCTCCACCGCCACAAGAAAACTCAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207567 representing NM_005815
Red=Cloning site Green=Tags(s)

MASVALEDVAVNFTREEWALLGPCQKNLYKDVMQETIRNLDCVVMKWKDQNIEDQYRYPRKNLRCRMLER
FVESKDGTQCGETSSQIQDSIVTKNTLPGVGPCESSMRGEKVMGHSSLNCYIRVGAGHKPHEYHECGEKP
DTHKQRGKAFSYHNSFQTHERLHTGKKPYDCKECGKSFSSLGNLQRHMAVQRGDGPYKCKLCGKAFFWPS
LLHMHERTHTGEKPYECKQCSKAFSFYSSYLRHERTHTGEKPYECKQCSKAFPFYSSYLRHERTHTGEKP
YKCKQCSKAFPDSSSCLIHERTHTGEKPYTCKQCGKAFSVSGSLQRHETTHSAEKPYACQQCGKAFHHLG
SFQRHMIRHTGNGPHKCKICGKGFDCPSSLQSHERTHTGEKPYECKQCGKALSHRSSFRSHMIMHTGDGP
HKCKVCGKAFVYPSVFQRHERTHTAEKPYKCKQCGKAYRISSSLRRHETTHTGEKPYKCKLGKAFIDFCS
FQNHKTTHTGEKPYECKECGKAFSRFRYLSRHKRTHTGEKPYECKTCRKAFGHYDNLKVHERIHSGEKPY
ECKECGKAFSWLTCFLRHERIHMREKSYECPQCGKAFTHSRFLQGHEKTHTGENPYECKECGKAFASLSS
LHRHKKTHWKKTHTGENPYECKECGKAFASLSSLHRHKKTH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005815
ORF Size 2013 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005815.1, NM_005815.2, NM_005815.3, NM_005815.4, NP_005806.1, NP_005806.2
RefSeq Size 2573 bp
RefSeq ORF 2016 bp
Locus ID 10224
UniProt ID Q9Y2A4
Cytogenetics 19p13.2
Domains KRAB, zf-C2H2
Protein Families Druggable Genome, Transcription Factors
MW 77.6 kDa
Summary Zinc finger proteins (ZNFs) bind DNA and, through this binding, regulate gene transcription. Most ZNFs contain conserved C2H2 motifs and are classified as Kruppel-type zinc fingers. For a general description of these proteins, see ZNF91 (MIM 603971).supplied by OMIM, Jul 2002
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"NM_005815" in other vectors (2)
SKU Description Size Price
RG207567 ZNF443 (tGFP-tagged) - Human zinc finger protein 443 (ZNF443). Note: ORF is codon optimized 10 ug
$814.00
SC116474 ZNF443 (untagged)-Human zinc finger protein 443 (ZNF443) 10 ug
$835.00

Plasmid Map for RC207567

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RC207567
Circular map for RC207567

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