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STARD8 (NM_014725) Human Tagged ORF Clone

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SKU
RC207530
STARD8 (Myc-DDK-tagged)-Human StAR-related lipid transfer (START) domain containing 8 (STARD8), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $891.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol STARD8
Synonyms ARHGAP38; DLC3; STARTGAP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207530 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCTTGAATAATTGTGCCTCGATGAAACTGGAGGTTCATTTTCAAAGCAAGCAGAATGAAGACTCAG
AAGAGGAAGAGCAGTGTACCATCAGTAGCCACTGGGCCTTCCAGCAGGAAAGTAAGTGCTGGTCTCCTAT
GGGGTCCTCTGATCTGTTGGCCCCACCGAGCCCTGGCCTGCCAGCGACCTCAAGCTGTGAGAGCGTCCTC
ACCGAGCTTAGTGCCACCTCTCTGCCAGTCATCACCGTGAGCCTACCACCCGAGCCAGCAGACTTGCCCT
TGCCAGGCCGTGCCCCCAGCTCGAGTGACCGGCCCCTCCTCAGCCCCACCCAGGGCCAGGAGGGTCCCCA
GGACAAAGCCAAGAAGCGCCATCGTAACCGTAGCTTCCTCAAGCACCTTGAATCTCTGAGGCGGAAGGAA
AAGAGTGGCAGCCAGCAAGCAGAGCCCAAGCATAGTCCAGCCACCTCAGAGAAGGTCTCCAAAGCCTCAT
CTTTCCGCAGTTGTCGTGGCTTCCTCTCAGCTGGATTTTACAGGGCCAAGAACTGGGCCGCCACCTCAGC
CGGTGGCAGTGGTGCCAATACTCGGAAGGCCTGGGAGGCCTGGCCTGTGGCCTCGTTCCGGCATCCTCAG
TGGACACACCGGGGTGATTGCCTGGTGCACGTTCCTGGGGACCACAAACCAGGCACATTCCCTCGCTCCC
TGTCCATTGAGAGCCTGTGTCCTGAGGATGGACACCGCCTGGCAGACTGGCAGCCAGGTAGGCGGTGGGG
CTGTGAGGGGCGCCGGGGCTCCTGTGGCTCAACGGGCAGCCATGCCAGCACGTATGACAACTTGCCTGAG
CTGTACCCAGCTGAGCCTGTAATGGTTGGGGCTGAGGCTGAAGATGAAGATGATGAGGAGAGTGGGGGCA
GCTATGCTCACCTAGACGACATCCTCCAGCACGTGTGGGGGCTACAGCAACGAGTAGAGCTGTGGTCTCG
GGCCATGTACCCAGACCTGGGGCCTGGAGATGAGGAAGAGGAGGAGGCCACTTCATCAGTAGAAATAGCC
ACAGTTGAGGTCAAATGCCAAGCTGAGGCTCTCAGCCAGATGGAGGTTCCGGCCCATGGAGAGTCCCCAG
CCTGGGCCCAGGCTGAAGTCCAGCCAGCAGTCCTGGCTCCGGCTCAGGCTCCAGCTGAGGCTGAACCAGT
GGCACAGGAAGAGGCTGAGGCCCCGGCCCCAGCCCCGGCCCCGGCCCCAGCCCAGGACAGTGAGCAGGAG
GCACATTCAGGCGGGGAACCCACCTTTGCCTCTAGCCTGTCTGTGGAAGAAGGACACTCCATTTCTGACA
CTGTGGCCTCCTCCAGCGAACTTGACAGTAGTGGGAACTCCATGAATGAGGCTGAGGCTGCGGGGCCCCT
GGCTGGACTCCAGGCATCAATGCCCCGTGAACGGCGCGATTCAGGTGTTGGGGCCTCACTTACCAGACCC
TGCAGGAAGCTCCGTTGGCATAGCTTCCAGAACTCCCATCGTCCCAGCCTCAACTCAGAGTCGCTGGAGA
TCAACCGGCAGTTTGCAGGCCAGATCAACCTCCTGCACAAGGGCTCACTGCTGCGGCTTACCGCGTTCAT
GGAGAAGTACACTGTGCCCCACAAGCAGGGCTGGGTCTGGTCAATGCCCAAGTTCATGAGGAGGAACAAG
ACCCCAGATTACCGGGGACAGCACGTATTTGGGGTGCCACCCCTCATCCACGTGCAGCGCACGGGCCAGC
CACTGCCACAGAGCATTCAGCAAGCCATGCGCTACTTGCGCAGCCAGTGCCTGGACCAAGTAGGCATCTT
CCGCAAGTCTGGGGTCAAGTCCAGGATCCAGAACCTGCGTCAAATGAATGAGACCTCGCCTGACAATGTC
TGCTACGAGGGCCAGTCAGCCTACGACGTGGCTGACCTGCTAAAGCAGTATTTCCGGGACCTGCCTGAGC
CCATCTTCACCAGCAAGCTCACCACCACTTTCCTCCAGATCTACCAGCTCCTCCCCAAGGATCAGTGGTT
GGCAGCAGCACAAGCCGCCACCTTGCTGCTCCCCGATGAGAACCGAGAGGTGCTACAGACCCTGCTCTAC
TTCTTAAGTGACATTGCCTCTGCCGAGGAAAACCAGATGACAGCAGGCAACCTGGCAGTGTGCCTGGCGC
CCTCCATCTTCCACCTCAATGTCTCTAAGAAGGATAGCCCCTCTCCCAGGATCAAGAGCAAACGCAGCCT
CATTGGCAGGCCAGGCCCTAGGGACCTGAGTGACAACATGGCAGCCACCCAGGGCCTGTCGCACATGATC
AGTGACTGCAAGAAACTTTTCCAGGTGCCCCAGGACATGGTGCTGCAGCTGTGCAGCTCCTACAGCGCAG
CTGAGCTCAGCCCTCCCGGCCCAGCCCTGGCTGAGCTGCGTCAGGCCCAAGCTGCAGGGGTAAGCCTGAG
CCTCTACATGGAAGAGAATATCCAGGACCTGCTGCGTGATGCTGCTGAGCGCTTCAAGGGCTGGATGAGC
GTGCCAGGGCCCCAGCACACGGAGCTGGCTTGCAGGAAGGCACCGGATGGGCACCCCCTGCGGCTATGGA
AGGCATCCACAGAGGTGGCAGCCCCCCCAGCTGTGGTGCTGCATCGTGTTCTCCGGGAGCGGGCCCTCTG
GGATGAGGATCTGCTGCGGGCCCAGGTGCTGGAAGCCCTGATGCCGGGTGTGGAGCTGTACCACTATGTC
ACCGACAGCATGGCACCCCATCCCTGCCGCGACTTTGTGGTGCTTCGGATGTGGCGCTCTGACCTGCCTC
GTGGGGGTTGCCTGCTTGTCTCCCAGTCCCTGGATCCGGAACAACCTGTGCCAGAGTCGGGTGTGCGAGC
CCTCATGCTCACATCCCAGTACCTCATGGAGCCTTGCGGCTTGGGCCGCTCTCGGCTCACACACATCTGC
CGGGCTGACCTCAGGGGCCGTTCTCCTGACTGGTACAACAAAGTCTTTGGACACCTGTGTGCCATGGAAG
TGGCAAAGATCCGGGACTCCTTCCCCACCCTGCAGGCAGCGGGCCCTGAGACAAAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207530 protein sequence
Red=Cloning site Green=Tags(s)

MTLNNCASMKLEVHFQSKQNEDSEEEEQCTISSHWAFQQESKCWSPMGSSDLLAPPSPGLPATSSCESVL
TELSATSLPVITVSLPPEPADLPLPGRAPSSSDRPLLSPTQGQEGPQDKAKKRHRNRSFLKHLESLRRKE
KSGSQQAEPKHSPATSEKVSKASSFRSCRGFLSAGFYRAKNWAATSAGGSGANTRKAWEAWPVASFRHPQ
WTHRGDCLVHVPGDHKPGTFPRSLSIESLCPEDGHRLADWQPGRRWGCEGRRGSCGSTGSHASTYDNLPE
LYPAEPVMVGAEAEDEDDEESGGSYAHLDDILQHVWGLQQRVELWSRAMYPDLGPGDEEEEEATSSVEIA
TVEVKCQAEALSQMEVPAHGESPAWAQAEVQPAVLAPAQAPAEAEPVAQEEAEAPAPAPAPAPAQDSEQE
AHSGGEPTFASSLSVEEGHSISDTVASSSELDSSGNSMNEAEAAGPLAGLQASMPRERRDSGVGASLTRP
CRKLRWHSFQNSHRPSLNSESLEINRQFAGQINLLHKGSLLRLTAFMEKYTVPHKQGWVWSMPKFMRRNK
TPDYRGQHVFGVPPLIHVQRTGQPLPQSIQQAMRYLRSQCLDQVGIFRKSGVKSRIQNLRQMNETSPDNV
CYEGQSAYDVADLLKQYFRDLPEPIFTSKLTTTFLQIYQLLPKDQWLAAAQAATLLLPDENREVLQTLLY
FLSDIASAEENQMTAGNLAVCLAPSIFHLNVSKKDSPSPRIKSKRSLIGRPGPRDLSDNMAATQGLSHMI
SDCKKLFQVPQDMVLQLCSSYSAAELSPPGPALAELRQAQAAGVSLSLYMEENIQDLLRDAAERFKGWMS
VPGPQHTELACRKAPDGHPLRLWKASTEVAAPPAVVLHRVLRERALWDEDLLRAQVLEALMPGVELYHYV
TDSMAPHPCRDFVVLRMWRSDLPRGGCLLVSQSLDPEQPVPESGVRALMLTSQYLMEPCGLGRSRLTHIC
RADLRGRSPDWYNKVFGHLCAMEVAKIRDSFPTLQAAGPETKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014725
ORF Size 3069 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014725.4
RefSeq Size 4880 bp
RefSeq ORF 3072 bp
Locus ID 9754
UniProt ID Q92502
Cytogenetics Xq13.1
Domains RhoGAP, START
MW 112.6 kDa
Summary This gene encodes a member of a subfamily of Rho GTPase activating proteins that contain a steroidogenic acute regulatory protein related lipid transfer domain. The encoded protein localizes to focal adhesions and may be involved in regulating cell morphology. This protein may also function as a tumor suppressor. provided by RefSeq, Mar 2010
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Documents
Resources
"NM_014725" in other vectors (5)
SKU Description Size Price
RC207530L3 Lenti ORF clone of Human StAR-related lipid transfer (START) domain containing 8 (STARD8), transcript variant 2, Myc-DDK-tagged 10 ug
$1,191.00
RC207530L4 Lenti ORF clone of Human StAR-related lipid transfer (START) domain containing 8 (STARD8), transcript variant 2, mGFP tagged 10 ug
$1,191.00
RG207530 STARD8 (tGFP-tagged) - Human StAR-related lipid transfer (START) domain containing 8 (STARD8), transcript variant 2 10 ug
$789.00 $1,091.00
SC114878 STARD8 (untagged)-Human StAR-related lipid transfer (START) domain containing 8 (STARD8), transcript variant 2 10 ug
$891.00
SC324312 STARD8 (untagged)-Human StAR-related lipid transfer (START) domain containing 8 (STARD8), transcript variant 2 10 ug
$891.00

Plasmid Map for RC207530

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207530
Circular map for RC207530

file-alt Citations

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