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NOP58 (NM_015934) Human Tagged ORF Clone

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SKU
RC207514
NOP58 (Myc-DDK-tagged)-Human NOP58 ribonucleoprotein homolog (yeast) (NOP58)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $492.00
In Stock*
Size
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "NOP58 or NM_015934"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NOP58
Synonyms HSPC120; NOP5; NOP5/NOP58
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207514 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGTGCTGTTTGAAACGTCTGTGGGTTACGCCATCTTTAAGGTTCTAAATGAGAAGAAACTTCAAG
AGGTTGATAGTTTATGGAAAGAATTTGAAACTCCAGAGAAAGCAAACAAAATAGTAAAGCTAAAACATTT
TGAGAAATTTCAGGATACAGCAGAAGCATTAGCAGCATTCACAGCTCTGATGGAGGGCAAAATCAATAAG
CAGCTGAAAAAAGTTCTGAAGAAAATAGTAAAAGAAGCCCATGAACCGCTGGCAGTAGCTGATGCTAAAC
TAGGAGGGGTCATAAAGGAAAAGCTGAATCTCAGTTGTATCCATAGTCCTGTTGTTAATGAACTTATGAG
AGGAATTCGTTCACAAATGGATGGATTAATCCCTGGGGTAGAACCACGTGAAATGGCAGCTATGTGTCTT
GGATTGGCTCACAGCCTGTCTCGATATAGATTGAAGTTTAGCGCTGATAAAGTAGACACAATGATTGTTC
AGGCAATTTCCTTGTTAGATGACTTGGATAAAGAACTAAACAACTACATTATGCGATGTAGAGAATGGTA
TGGCTGGCATTTCCCTGAATTAGGAAAAATTATTTCAGATAATTTAACATACTGCAAGTGTTTACAGAAA
GTTGGCGATAGGAAGAACTATGCCTCTGCCAAGCTTTCTGAGTTGCTGCCAGAAGAAGTTGAAGCAGAAG
TGAAAGCAGCTGCAGAGATATCAATGGGAACAGAGGTTTCAGAAGAAGATATTTGCAATATTCTGCATCT
TTGCACCCAGGTGATTGAAATCTCTGAATATCGAACCCAGCTCTATGAATATCTACAAAATCGAATGATG
GCCATTGCACCCAATGTTACAGTCATGGTTGGGGAATTAGTTGGAGCACGGCTTATTGCTCATGCAGGTT
CTCTTTTAAATTTGGCCAAGCATGCAGCTTCTACCGTTCAGATTCTTGGAGCTGAAAAGGCACTTTTCAG
AGCCCTCAAATCTAGACGGGATACCCCTAAGTATGGTCTCATTTATCATGCTTCACTCGTGGGCCAGACA
AGTCCCAAACACAAAGGAAAGATTTCTCGAATGCTGGCAGCCAAAACCGTTTTGGCTATCCGTTATGATG
CTTTTGGTGAGGATTCAAGTTCTGCAATGGGAGTTGAGAACAGAGCCAAATTAGAGGCCAGGTTGAGAAC
TTTGGAAGACAGAGGGATAAGAAAAATAAGTGGAACAGGAAAAGCATTAGCAAAAACAGAAAAATATGAA
CACAAAAGTGAAGTGAAGACTTACGATCCTTCTGGTGACTCCACACTTCCAACCTGTTCTAAAAAACGCA
AAATAGAACAGGTAGATAAAGAGGATGAAATTACTGAAAAGAAAGCCAAAAAAGCCAAGATTAAAGTTAA
AGTTGAAGAAGAGGAAGAAGAAAAAGTGGCAGAAGAAGAAGAAACATCTGTGAAGAAGAAGAAGAAAAGG
GGTAAAAAGAAACACATTAAGGAAGAACCACTTTCTGAGGAAGAACCATGTACCAGCACAGCAATTGCTA
GTCCAGAGAAAAAGAAGAAAAAGAAAAAAAAGAGAGAGAACGAGGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207514 protein sequence
Red=Cloning site Green=Tags(s)

MLVLFETSVGYAIFKVLNEKKLQEVDSLWKEFETPEKANKIVKLKHFEKFQDTAEALAAFTALMEGKINK
QLKKVLKKIVKEAHEPLAVADAKLGGVIKEKLNLSCIHSPVVNELMRGIRSQMDGLIPGVEPREMAAMCL
GLAHSLSRYRLKFSADKVDTMIVQAISLLDDLDKELNNYIMRCREWYGWHFPELGKIISDNLTYCKCLQK
VGDRKNYASAKLSELLPEEVEAEVKAAAEISMGTEVSEEDICNILHLCTQVIEISEYRTQLYEYLQNRMM
AIAPNVTVMVGELVGARLIAHAGSLLNLAKHAASTVQILGAEKALFRALKSRRDTPKYGLIYHASLVGQT
SPKHKGKISRMLAAKTVLAIRYDAFGEDSSSAMGVENRAKLEARLRTLEDRGIRKISGTGKALAKTEKYE
HKSEVKTYDPSGDSTLPTCSKKRKIEQVDKEDEITEKKAKKAKIKVKVEEEEEEKVAEEEETSVKKKKKR
GKKKHIKEEPLSEEEPCTSTAIASPEKKKKKKKKRENED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015934
ORF Size 1587 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015934.5
RefSeq Size 1979 bp
RefSeq ORF 1590 bp
Locus ID 51602
UniProt ID Q9Y2X3
Cytogenetics 2q33.1
Domains Nop
MW 59.6 kDa
Summary The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs. provided by RefSeq, Nov 2015
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Documents
Resources
"NM_015934" in other vectors (4)
SKU Description Size Price
RC207514L3 Lenti ORF clone of Human NOP58 ribonucleoprotein homolog (yeast) (NOP58), Myc-DDK-tagged 10 ug
$792.00
RC207514L4 Lenti ORF clone of Human NOP58 ribonucleoprotein homolog (yeast) (NOP58), mGFP tagged 10 ug
$792.00
RG207514 NOP58 (tGFP-tagged) - Human NOP58 ribonucleoprotein homolog (yeast) (NOP58) 10 ug
$489.00 $692.00
SC321742 NOP58 (untagged)-Human NOP58 ribonucleoprotein homolog (yeast) (NOP58) 10 ug
$493.00

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