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Centaurin alpha 2 (ADAP2) (NM_018404) Human Tagged ORF Clone

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SKU
RC207501
ADAP2 (Myc-DDK-tagged)-Human ArfGAP with dual PH domains 2 (ADAP2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Centaurin alpha 2 or NM_018404"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Centaurin alpha 2
Synonyms cent-b; CENTA2; HSA272195
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207501 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCCC

ATGGGCGATCGCGAGCGCAACAAGAAGCGGCTGCTGGAGCTGCTGCGGGCGCCGGACACAGGCAACGCGC
ACTGCGCCGACTGCGGGGCGGCAGATCCCGACTGGGCCTCTTACAAGCTGGGGATCTTCATCTGTCTCAA
CTGCTGCGGCGTCCACCGTAACTTCCCTGACATCAGCAGAGTTAAATCTGTGCGACTTGACTTCTGGGAC
GACAGTATTGTGGAGTTTATGATCCACAATGGAAACCTCCGTGTGAAGGCCAAGTTCGAAGCCAGAGTCC
CAGCTTTCTACTACATCCCCCAGGCCAACGACTGCCTGGTCTTAAAGGAACAATGGATTCGAGCTAAGTA
TGAGAGACGGGAATTTATGGCTGATGGGGAAACCATCTCGCTCCCAGGTAACCGAGAAGGATTCCTGTGG
AAGCGAGGAAGGGACAACTCACAGTTTCTGAGAAGGAAGTTTGTACTTCTGGCAAGAGAAGGCCTCCTGA
AGTACTTCACAAAGGAACAGGGTAAAAGCCCCAAAGCTGTCATCAGCATTAAGGACTTGAATGCCACCTT
CCAGACAGAGAAGATAGGGCACCCCCATGGGCTGCAGATCACCTACAGGAGAGATGGCCACACCAGGAAC
CTGTTTGTGTATCATGAAAGTGGGAAGGAGATAGTGGACTGGTTCAATGCCCTCCGTGCAGCCCGTCTGC
AGTACCTAAAAATGGCCTTTCCTGAACTCCCAGAGTCTGAGCTCGTGCCATTCCTCACCAGGAACTACCT
CAAACAAGGCTTCATGGAAAAGACTGGGCCAAAGAAAGAACCTTTCAAGAAAAGGTGGTTCGCCCTGGAT
TGCCATGAGCGGAGGCTGCTCTATTACAAGAACCCACTGGATGCCTTCGAGCAGGGCCAGGTTTTTCTTG
GGAACAAGGAGCAGGGATATGAAGCCTACGAAGACCTGCCCAAGGGCATCCGAGGAAATCGCTGGAAAGC
CGGACTCACCATTGTCACCCCAGAGCGGAGATTTGTCCTCACTTGCCCCAGTGAGAAGGAACAGCAGGAA
TGGCTGGAAAGTTTGCGGGGTGTCCTGTCCAGCCCCTTGACGCCCCTCAACCGGCTTACTGCATCAACAG
AGAGTGGCCGCAGCAGCAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207501 protein sequence
Red=Cloning site Green=Tags(s)

MGDRERNKKRLLELLRAPDTGNAHCADCGAADPDWASYKLGIFICLNCCGVHRNFPDISRVKSVRLDFWD
DSIVEFMIHNGNLRVKAKFEARVPAFYYIPQANDCLVLKEQWIRAKYERREFMADGETISLPGNREGFLW
KRGRDNSQFLRRKFVLLAREGLLKYFTKEQGKSPKAVISIKDLNATFQTEKIGHPHGLQITYRRDGHTRN
LFVYHESGKEIVDWFNALRAARLQYLKMAFPELPESELVPFLTRNYLKQGFMEKTGPKKEPFKKRWFALD
CHERRLLYYKNPLDAFEQGQVFLGNKEQGYEAYEDLPKGIRGNRWKAGLTIVTPERRFVLTCPSEKEQQE
WLESLRGVLSSPLTPLNRLTASTESGRSSR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018404
ORF Size 1140 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018404.3
RefSeq Size 2757 bp
RefSeq ORF 1146 bp
Locus ID 55803
UniProt ID Q9NPF8
Cytogenetics 17q11.2
Domains ArfGap, PH
MW 44.2 kDa
Summary The protein encoded by this gene binds beta-tubulin and increases the stability of microtubules. The encoded protein can also translocate to the cell membrane and bind phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). In addition, this protein is a GTPase-activating protein for ADP ribosylation factor 6 and may be able to block the entry of some RNA viruses. provided by RefSeq, Oct 2016
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"NM_018404" in other vectors (4)
SKU Description Size Price
RC207501L3 Lenti ORF clone of Human ArfGAP with dual PH domains 2 (ADAP2), Myc-DDK-tagged 10 ug
$757.00
RC207501L4 Lenti ORF clone of Human ArfGAP with dual PH domains 2 (ADAP2), mGFP tagged 10 ug
$757.00
RG207501 ADAP2 (tGFP-tagged) - Human ArfGAP with dual PH domains 2 (ADAP2) 10 ug
$657.00
SC113495 ADAP2 (untagged)-Human ArfGAP with dual PH domains 2 (ADAP2) 10 ug
$457.00

Plasmid Map for RC207501

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207501
Circular map for RC207501

file-alt Citations

LY6E Restricts Entry of Human Coronaviruses, Including Currently Pandemic SARS-CoV-2
Zhao, Zheng, Chen et al
J Virol (2020) 94 (18) DOI: 10.1128/JVI.00562-20
AI Summary: In this study, OriGene products including the LY6E (RC208447) and GILT (RC217745) expression plasmids were used to investigate the roles of these proteins in restricting human coronavirus entry, demonstrating that overexpression of LY6E inhibits infection by multiple coronaviruses including SARS-CoV-2.
FigureFigureFigure
Showing 1-1 of 1 papers.
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