F8A2 (NM_001007523) Human Tagged ORF Clone

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SKU
RC207473
F8A2 (Myc-DDK-tagged)-Human coagulation factor VIII-associated 2 (F8A2)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol F8A2
Synonyms HAP40
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207473 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGCGGCTGCAGGCCTGGGCGGCGGCGGCGCCGGCCCGGGACCCGAGGCCGGGGACTTCCTGG
CCCGCTACCGGCTGGTATCGAACAAGCTGAAGAAGCGGTTCCTGCGGAAGCCGAACGTGGCGGAGGCCGG
CGAGCAGTTCGGACAGCTGGGCCGGGAGCTGCGCGCCCAGGAGTGTCTGCCCTACGCGGCCTGGTGCCAG
CTGGCGGTGGCGCGCTGCCAGCAGGCGCTCTTCCACGGGCCCGGGGAGGCGCTGGCCCTCACCGAGGCCG
CCCGCCTCTTCCTGCGGCAGGAGCGCGACGCGCGCCAGCGCCTGGTCTGCCCCGCCGCCTACGGGGAGCC
GCTGCAGGCCGCCGCCAGCGCCCTGGGCGCCGCGGTGCGTCTGCACCTCGAGCTGGGCCAGCCGGCCGCC
GCCGCCGCCCTCTGCCTCGAGCTGGCCGCCGCCCTGCGCGACCTGGGCCAGCCGGCCGCCGCCGCCGGTC
ACTTCCAGCGCGCCGCCCAGCTCCAGCTGCCCCAGCTGCCCCTGGCCGCGCTGCAGGCGCTTGGCGAGGC
CGCCTCCTGCCAGCTGCTGGCGCGCGACTACACCGGCGCCCTGGCGGTCTTCACGCGCATGCAGCGCCTG
GCGCGGGAGCACGGCAGCCACCCGGTGCAGTCACTGCCGCCGCCCCCGCCGCCGGCACCCCAGCCCGGGC
CCGGGGCGACGCCCGCCCTACCGGCCGCGCTGCTTCCTCCGAACTCCGGCTCGGCGGCGCCCTCTCCCGC
CGCCCTGGGCGCCTTCTCGGACGTGCTGGTCCGCTGCGAGGTGTCCCGCGTGCTGCTGCTGCTCCTCCTG
CAACCACCGCCCGCCAAGCTGCTGCCGGAGCACGCCCAGACCCTGGAGAAGTACTCCTGGGAGGCTTTTG
ACAGCCACGGGCAGGAGAGCAGCGGCCAGCTTCCCGAGGAGCTCTTTCTGCTGCTCCAGTCTTTGGTCAT
GGCTACCCACGAAAAGGACACGGAAGCCATCAAGTCGCTGCAGGTGGAGATGTGGCCACTGTTGACTGCT
GAGCAGAACCACCTCCTTCACCTCGTTCTGCAAGAAACCATCTCCCCCTCAGGACAGGGAGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207473 protein sequence
Red=Cloning site Green=Tags(s)

MAAAAAGLGGGGAGPGPEAGDFLARYRLVSNKLKKRFLRKPNVAEAGEQFGQLGRELRAQECLPYAAWCQ
LAVARCQQALFHGPGEALALTEAARLFLRQERDARQRLVCPAAYGEPLQAAASALGAAVRLHLELGQPAA
AAALCLELAAALRDLGQPAAAAGHFQRAAQLQLPQLPLAALQALGEAASCQLLARDYTGALAVFTRMQRL
AREHGSHPVQSLPPPPPPAPQPGPGATPALPAALLPPNSGSAAPSPAALGAFSDVLVRCEVSRVLLLLLL
QPPPAKLLPEHAQTLEKYSWEAFDSHGQESSGQLPEELFLLLQSLVMATHEKDTEAIKSLQVEMWPLLTA
EQNHLLHLVLQETISPSGQGV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001007523
ORF Size 1113 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001007523.1, NP_001007524.1
RefSeq Size 1116 bp
RefSeq ORF 1116 bp
Locus ID 474383
UniProt ID P23610
Cytogenetics Xq28
MW 39.1 kDa
Summary This gene is part of a region that is repeated three times on chromosome X, once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the middle copy. Although its function is unknown, the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII, this gene is transcribed abundantly in a wide variety of cell types. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001007523" in other vectors (4)
SKU Description Size Price
RC207473L3 Lenti ORF clone of Human coagulation factor VIII-associated 2 (F8A2), Myc-DDK-tagged 10 ug
$757.00
RC207473L4 Lenti ORF clone of Human coagulation factor VIII-associated 2 (F8A2), mGFP tagged 10 ug
$757.00
RG207473 F8A2 (tGFP-tagged) - Human coagulation factor VIII-associated 2 (F8A2) 10 ug
$657.00
SC301219 F8A2 (untagged)-Human coagulation factor VIII-associated 2 (F8A2) 10 ug
$503.00

Plasmid Map for RC207473

Download sequence for

Cloning scheme for RC207473

Circular map for RC207473

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