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USP46 (NM_022832) Human Tagged ORF Clone

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SKU
RC207470
USP46 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 46 (USP46), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol USP46
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207470 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGTCCGAAACATCGCCTCCATCTGTAATATGGGCACCAATGCCTCTGCTCTGGAAAAAGACATTG
GTCCAGAGCAGTTTCCAATCAATGAACACTATTTCGGATTGGTCAATTTTGGAAACACATGCTACTGTAA
CTCCGTGCTTCAGGCATTGTACTTCTGCCGTCCATTCCGGGAGAATGTGTTGGCATACAAGGCCCAGCAA
AAGAAGAAGGAAAACTTGCTGACGTGCCTGGCGGACCTTTTCCACAGCATTGCCACACAGAAGAAGAAGG
TTGGCGTCATCCCACCAAAGAAGTTCATTTCAAGGCTGAGAAAAGAGAATGATCTCTTTGATAACTACAT
GCAGCAGGATGCTCATGAATTTTTAAATTATTTGCTAAACACTATTGCGGACATCCTTCAGGAGGAGAAG
AAACAGGAAAAACAAAATGGAAAATTAAAAAATGGCAACATGAACGAACCTGCGGAAAATAATAAACCAG
AACTCACCTGGGTCCATGAGATTTTTCAGGGAACGCTTACCAATGAAACTCGATGCTTGAACTGTGAAAC
TGTTAGTAGCAAAGATGAAGATTTTCTTGACCTTTCTGTTGATGTGGAGCAGAATACATCCATTACCCAC
TGTCTAAGAGACTTCAGCAACACAGAAACACTGTGTAGTGAACAAAAATATTATTGTGAAACATGCTGCA
GCAAACAAGAAGCCCAGAAAAGGATGAGGGTAAAAAAGCTGCCCATGATCTTGGCCCTGCACCTAAAGCG
GTTCAAGTACATGGAGCAGCTGCACAGATACACCAAGCTGTCTTACCGTGTGGTCTTCCCTCTGGAACTC
CGGCTCTTCAACACCTCCAGTGATGCAGTGAACCTGGACCGCATGTATGACTTGGTTGCGGTGGTCGTTC
ACTGTGGCAGTGGTCCTAATCGTGGGCATTATATCACTATTGTGAAAAGTCACGGCTTCTGGCTTTTGTT
TGATGATGACATTGTAGAGAAAATAGATGCTCAAGCTATTGAAGAATTCTATGGCCTGACGTCAGATATA
TCAAAAAATTCAGAATCTGGATATATTTTATTCTATCAGTCAAGAGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207470 protein sequence
Red=Cloning site Green=Tags(s)

MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQ
KKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEK
KQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITH
CLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLEL
RLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDI
SKNSESGYILFYQSRE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022832
ORF Size 1098 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022832.4
RefSeq Size 7981 bp
RefSeq ORF 1101 bp
Locus ID 64854
UniProt ID P62068
Cytogenetics 4q12
Domains UCH
Protein Families Druggable Genome, Protease
MW 42.4 kDa
Summary Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 PubMed 14715245).supplied by OMIM, Jun 2009
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Documents
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"NM_022832" in other vectors (6)
SKU Description Size Price
RC207470L1 Lenti ORF clone of Human ubiquitin specific peptidase 46 (USP46), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC207470L2 Lenti ORF clone of Human ubiquitin specific peptidase 46 (USP46), transcript variant 1, mGFP tagged 10 ug
$757.00
RC207470L3 Lenti ORF clone of Human ubiquitin specific peptidase 46 (USP46), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC207470L4 Lenti ORF clone of Human ubiquitin specific peptidase 46 (USP46), transcript variant 1, mGFP tagged 10 ug
$757.00
RG207470 USP46 (tGFP-tagged) - Human ubiquitin specific peptidase 46 (USP46), transcript variant 1 10 ug
$489.00 $657.00
SC126495 USP46 (untagged)-Human ubiquitin specific peptidase 46 (USP46), transcript variant 1 10 ug
$457.00

Plasmid Map for RC207470

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207470
Circular map for RC207470

file-alt Citations

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