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THNSL1 (NM_024838) Human Tagged ORF Clone

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SKU
RC207320
THNSL1 (Myc-DDK-tagged)-Human threonine synthase-like 1 (S. cerevisiae) (THNSL1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $680.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol THNSL1
Synonyms TSH1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207320 representing NM_024838
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCACTTTAACCGATGTCATCATCTGAAAAAGATAACACAGAAATGTTTTTCTAGTATACATGTTA
AAACGGATAAACATGCACAGCGATTTCTTTCAAGAACCTTTGCACTTGCGGAATTGAGGAAGTCATGGTA
TTCAACCCACTCTCTTGTTGGAGACAAAAATATTATCCTGATGGGACCTCCTGGTGCTGGGAAAACAACA
GTAGGCAGAATAATAGGTCAGAAACTAGGTTGTTGTGTCATAGATGTGGATGATGATATCCTTGAAAAAA
CCTGGAATATGAGTGTGTCTGAAAAATTACAGGATGTTGGTAATGAGCAATTTTTAGAAGAGGAAGGAAA
AGCTGTGTTAAACTTCTCTGCATCTGGAAGTGTGATTTCCCTTACTGGGTCCAATCCAATGCATGATGCT
AGCATGTGGCATCTGAAGAAAAATGGAATAATTGTATACCTGGATGTACCTCTACTAGATCTAATTTGTC
GTCTAAAATTAATGAAGACAGATAGGATTGTAGGTCAGAATTCTGGAACATCTATGAAAGACTTACTTAA
ATTTAGAAGACAGTATTATAAGAAGTGGTATGATGCTCGTGTTTTCTGTGAAAGTGGGGCTTCCCCAGAG
GAGGTAGCTGACAAAGTGCTGAATGCAATTAAAAGATACCAAGATGTGGACTCGGAAACATTCATTTCAA
CAAGACACGTTTGGCCTGAAGACTGTGAACAGAAGGTTTCAGCAAAATTCTTTAGTGAAGCTGTAATTGA
GGGGTTGGCTTCTGATGGTGGCCTCTTTGTTCCTGCAAAGGAGTTTCCAAAATTAAGCTGCGGGGAGTGG
AAAAGCCTAGTAGGAGCAACCTACGTAGAAAGAGCACAGATACTGTTGGAAAGATGTATCCATCCTGCAG
ACATACCTGCTGCCAGGTTGGGAGAAATGATTGAAACTGCTTATGGGGAAAACTTTGCCTGCTCAAAAAT
TGCTCCTGTCAGGCACCTTTCAGGCAACCAGTTCATCCTGGAGTTGTTTCATGGACCAACAGGATCATTT
AAAGATTTGTCTTTACAGCTTATGCCTCATATTTTTGCACACTGTATCCCACCAAGTTGCAATTATATGA
TACTTGTAGCTACTTCAGGAGACACAGGGAGTGCAGTCTTAAATGGTTTTAGTCGTCTAAATAAGAATGA
TAAGCAAAGGATAGCTGTGGTTGCATTTTTTCCTGAGAATGGAGTAAGTGATTTTCAAAAAGCACAAATA
ATTGGCAGTCAGAGAGAAAATGGATGGGCAGTGGGTGTTGAGTCAGATTTTGATTTTTGCCAGACAGCTA
TAAAAAGAATTTTTAATGATTCTGATTTTACTGGCTTTCTTACTGTGGAATATGGAACAATCTTAAGTTC
GGCTAACTCCATAAACTGGGGCCGACTACTTCCGCAGGTAGTTTATCATGCTTCCGCATATCTTGATCTT
GTTAGTCAAGGATTTATTTCTTTTGGAAGCCCAGTCGATGTCTGTATTCCCACAGGAAACTTTGGTAACA
TTTTAGCAGCAGTGTATGCCAAAATGATGGGAATCCCGATTCGAAAATTTATCTGTGCCTCTAATCAGAA
CCATGTTTTGACTGATTTTATAAAAACAGGACATTATGATCTAAGGGAAAGAAAACTAGCACAAACCTTT
TCACCGTCAATAGATATTCTCAAATCTTCAAACCTAGAACGACATTTACACTTGATGGCTAATAAAGATG
GACAGCTAATGACAGAATTATTTAATCGATTAGAAAGTCAGCATCATTTCCAGATAGAAAAGGCTCTAGT
TGAGAAACTTCAGCAGGATTTTGTAGCTGACTGGTGCTCTGAGGGAGAGTGCCTAGCAGCTATTAACTCC
ACCTATAATACTTCAGGGTATATTTTGGATCCACACACTGCTGTTGCAAAAGTGGTTGCAGATAGGGTGC
AAGACAAAACTTGCCCTGTGATTATCTCATCTACAGCCCATTACTCAAAGTTTGCACCTGCTATCATGCA
GGCTTTAAAGATTAAAGAAATCAATGAGACTTCATCAAGTCAGCTCTATTTGCTGGGTTCATACAATGCA
TTACCTCCACTGCATGAGGCTTTATTAGAGAGAACAAAACAGCAAGAGAAGATGGAGTACCAGGTCTGTG
CAGCTGATATGAATGTCTTGAAGAGTCATGTGGAACAACTTGTCCAAAATCAATTCATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207320 representing NM_024838
Red=Cloning site Green=Tags(s)

MLHFNRCHHLKKITQKCFSSIHVKTDKHAQRFLSRTFALAELRKSWYSTHSLVGDKNIILMGPPGAGKTT
VGRIIGQKLGCCVIDVDDDILEKTWNMSVSEKLQDVGNEQFLEEEGKAVLNFSASGSVISLTGSNPMHDA
SMWHLKKNGIIVYLDVPLLDLICRLKLMKTDRIVGQNSGTSMKDLLKFRRQYYKKWYDARVFCESGASPE
EVADKVLNAIKRYQDVDSETFISTRHVWPEDCEQKVSAKFFSEAVIEGLASDGGLFVPAKEFPKLSCGEW
KSLVGATYVERAQILLERCIHPADIPAARLGEMIETAYGENFACSKIAPVRHLSGNQFILELFHGPTGSF
KDLSLQLMPHIFAHCIPPSCNYMILVATSGDTGSAVLNGFSRLNKNDKQRIAVVAFFPENGVSDFQKAQI
IGSQRENGWAVGVESDFDFCQTAIKRIFNDSDFTGFLTVEYGTILSSANSINWGRLLPQVVYHASAYLDL
VSQGFISFGSPVDVCIPTGNFGNILAAVYAKMMGIPIRKFICASNQNHVLTDFIKTGHYDLRERKLAQTF
SPSIDILKSSNLERHLHLMANKDGQLMTELFNRLESQHHFQIEKALVEKLQQDFVADWCSEGECLAAINS
TYNTSGYILDPHTAVAKVVADRVQDKTCPVIISSTAHYSKFAPAIMQALKIKEINETSSSQLYLLGSYNA
LPPLHEALLERTKQQEKMEYQVCAADMNVLKSHVEQLVQNQFI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024838
ORF Size 2229 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024838.5
RefSeq Size 3787 bp
RefSeq ORF 2232 bp
Locus ID 79896
UniProt ID Q8IYQ7, Q9H6P9
Cytogenetics 10p12.1
MW 83.1 kDa
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"NM_024838" in other vectors (4)
SKU Description Size Price
RC207320L3 Lenti-ORF clone of THNSL1 (Myc-DDK-tagged)-Human threonine synthase-like 1 (S. cerevisiae) (THNSL1) 10 ug
$980.00
RC207320L4 Lenti-ORF clone of THNSL1 (mGFP-tagged)-Human threonine synthase-like 1 (S. cerevisiae) (THNSL1) 10 ug
$980.00
RG207320 THNSL1 (tGFP-tagged) - Human threonine synthase-like 1 (S. cerevisiae) (THNSL1) 10 ug
$880.00
SC112007 THNSL1 (untagged)-Human threonine synthase-like 1 (S. cerevisiae) (THNSL1) 10 ug
$681.00

Plasmid Map for RC207320

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207320
Circular map for RC207320

file-alt Citations

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