OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

LRWD1 (NM_152892) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC207141
LRWD1 (Myc-DDK-tagged)-Human leucine-rich repeats and WD repeat domain containing 1 (LRWD1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $602.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA371036 LRWD1 rabbit polyclonal antibody
100 ul
$310.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "LRWD1 or NM_152892"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRWD1
Synonyms CENP-33; ORCA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207141 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCCCCTCTCGGCGCGGCTGCTAATGCAGCGCGGGCGCCCCAAGAGCGACCGGCTGGGGAAGATCC
GGAGTCTGGACCTGTCAGGATTGGAGCTGCTTTCCGAGCACCTGGACCCCAAACTCCTGTGCCGCCTGAC
GCAGCTGCAGGAGCTTGACCTGTCTAACAACCACCTGGAGACGCTGCCGGACAACCTGGGCCTGTCCCAC
CTGCGTGTCCTCCGCTGCGCCAACAACCAGCTGGGGGATGTTACTGCCTTGTGCCAGTTCCCCAAGCTCG
AGGAACTCAGCCTGGAGGGCAACCCCTTCCTGACGGTCAATGACAACCTGAAAGTCTCCTTTCTCCTGCC
CACGCTCCGTAAGGTCAATGGCAAGGATGCGTCCTCAACTTACTCTCAGGTGGAGAACCTGAATCGGGAG
CTGACCAGCAGGGTCACAGCTCACTGGGAGAAGTTCATGGCCACACTGGGTCCTGAAGAGGAGGCTGAGA
AGGCCCAGGCGGACTTTGTGAAGTCGGCTGTCAGGGATGTCCGCTACGGGCCCGAGTCCCTCAGCGAGTT
CACCCAGTGGCGGGTGCGGATGATCTCTGAGGAGCTGGTGGCCGCCAGTAGGACCCAGGTGCAAAAGGCT
AACAGCCCAGAGAAGCCCCCAGAAGCTGGAGCTGCCCACAAGCCCAGGGCCAGACTGGCGGCCTTGAAAC
GGCCAGACGACGTCCCACTCAGCCTCTCTCCCAGCAAGCGGGCGTGTGCCTCCCCGTCGGCCCAGGTGGA
GGGCAGCCCTGTGGCAGGCTCCGATGGCAGCCAGCCTGCTGTGAAGCTGGAGCCCCTGCACTTCCTGCAG
TGCCACAGCAAGAACAACAGCCCCCAGGACCTCGAGACCCAGCTGTGGGCCTGTGCCTTCGAGCCGGCCT
GGGAGGAGGGGGCCACATCCCAGACCGTGGCCACGTGCGGCGGGGAGGCTGTGTGCGTAATTGATTGCCA
GACGGGCATCGTGCTCCACAAGTACAAGGCACCCGGCGAGGAGTTCTTTTCTGTGGCCTGGACCGCTCTG
ATGGTGGTCACACAGGCTGGCCACAAGAAGCGCTGGAGTGTGCTGGCGGCTGCAGGCCTACGGGGCCTGG
TCCGGCTGCTGCACGTGCGTGCCGGCTTCTGCTGCGGGGTCATCCGAGCCCACAAGAAGGCCATCGCCAC
CCTGTGCTTCAGCCCCGCCCACGAGACCCATCTCTTCACGGCCTCCTATGACAAGCGGATCATCCTCTGG
GACATCGGGGTGCCCAACCAGGACTACGAATTCCAGGCCAGCCAGCTGCTCACACTGGACACCACCTCTA
TCCCCCTGCGCCTCTGCCCTGTCGCCTCCTGCCCGGACGCCCGCCTGCTGGCCGGCTGCGAGGGCGGCTG
CTGCTGCTGGGACGTGCGGCTGGACCAGCCCCAAAAGAGGAGGGTGTGTGAAGTGGAATTCGTCTTCTCT
GAGGGCTCCGAGGCATCTGGACGGAGAGTGGATGGGCTGGCATTTGTGAATGAGGACATCGTGGCCTCCA
AGGGGAGCGGCCTGGGCACCATCTGCCTGTGGAGCTGGAGGCAGACGTGGGGGGGCCGGGGCAGCCAGTC
CACGGTGGCAGTGGTGGTCCTGGCGCGGCTGCAATGGTCGTCCACCGAGTTGGCCTACTTCTCGCTCAGC
GCCTGCCCTGATAAGGGGATTGTGCTCTGTGGGGATGAGGAGGGCAACGTGTGGCTCTACGACGTCAGCA
ACATCCTGAAGCAGCCACCCCTGCTGCCGGCAGCCCTGCAGGCCCCCACACAGATCCTGAAGTGGCCCCA
GCCCTGGGCCCTTGGCCAGGTGGTGACCAAGACCATGGTGAACACAGTGGTGGCCAATGCCTCCTTCACC
TACCTCACCGCCCTGACGGACTCCAACATCGTAGCCATCTGGGGGAGGATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207141 protein sequence
Red=Cloning site Green=Tags(s)

MGPLSARLLMQRGRPKSDRLGKIRSLDLSGLELLSEHLDPKLLCRLTQLQELDLSNNHLETLPDNLGLSH
LRVLRCANNQLGDVTALCQFPKLEELSLEGNPFLTVNDNLKVSFLLPTLRKVNGKDASSTYSQVENLNRE
LTSRVTAHWEKFMATLGPEEEAEKAQADFVKSAVRDVRYGPESLSEFTQWRVRMISEELVAASRTQVQKA
NSPEKPPEAGAAHKPRARLAALKRPDDVPLSLSPSKRACASPSAQVEGSPVAGSDGSQPAVKLEPLHFLQ
CHSKNNSPQDLETQLWACAFEPAWEEGATSQTVATCGGEAVCVIDCQTGIVLHKYKAPGEEFFSVAWTAL
MVVTQAGHKKRWSVLAAAGLRGLVRLLHVRAGFCCGVIRAHKKAIATLCFSPAHETHLFTASYDKRIILW
DIGVPNQDYEFQASQLLTLDTTSIPLRLCPVASCPDARLLAGCEGGCCCWDVRLDQPQKRRVCEVEFVFS
EGSEASGRRVDGLAFVNEDIVASKGSGLGTICLWSWRQTWGGRGSQSTVAVVVLARLQWSSTELAYFSLS
ACPDKGIVLCGDEEGNVWLYDVSNILKQPPLLPAALQAPTQILKWPQPWALGQVVTKTMVNTVVANASFT
YLTALTDSNIVAIWGRM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152892
ORF Size 1941 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152892.3
RefSeq Size 2220 bp
RefSeq ORF 1944 bp
Locus ID 222229
UniProt ID Q9UFC0, A0A140VJD0
Cytogenetics 7q22.1
Domains LRR, WD40
Protein Families Druggable Genome
MW 70.9 kDa
Summary The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Dec 2015
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_152892" in other vectors (4)
SKU Description Size Price
RC207141L3 Lenti ORF clone of Human leucine-rich repeats and WD repeat domain containing 1 (LRWD1), Myc-DDK-tagged 10 ug
$902.00
RC207141L4 Lenti ORF clone of Human leucine-rich repeats and WD repeat domain containing 1 (LRWD1), mGFP tagged 10 ug
$902.00
RG207141 LRWD1 (tGFP-tagged) - Human leucine-rich repeats and WD repeat domain containing 1 (LRWD1) 10 ug
$802.00
SC108154 LRWD1 (untagged)-Human leucine-rich repeats and WD repeat domain containing 1 (LRWD1) 10 ug
$603.00

Plasmid Map for RC207141

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207141
Circular map for RC207141

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products