TMEM63A (NM_014698) Human Tagged ORF Clone
$739.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TMEM63A |
| Synonyms | HLD19; KIAA0792 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014698 |
| ORF Size | 2421 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014698.3 |
| RefSeq Size | 4469 bp |
| RefSeq ORF | 2424 bp |
| Locus ID | 9725 |
| UniProt ID | O94886, A1NY77 |
| Cytogenetics | 1q42.12 |
| Domains | DUF221 |
| Protein Families | Transmembrane |
| MW | 92.1 kDa |
| Summary | Acts as an osmosensitive calcium-permeable cation channel.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014698" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206992L3 | Lenti ORF clone of Human transmembrane protein 63A (TMEM63A), Myc-DDK-tagged | 10 ug |
$1,039.00
|
|
| RC206992L4 | Lenti ORF clone of Human transmembrane protein 63A (TMEM63A), mGFP tagged | 10 ug |
$1,039.00
|
|
| RG206992 | TMEM63A (tGFP-tagged) - Human transmembrane protein 63A (TMEM63A) | 10 ug |
$789.00
$939.00
|
|
| SC108719 | TMEM63A (untagged)-Human transmembrane protein 63A (TMEM63A) | 10 ug |
$740.00
|
|
| SC324187 | TMEM63A (untagged)-Human transmembrane protein 63A (TMEM63A) | 10 ug |
$740.00
|
|
Citations
Heterozygous Variants in the Mechanosensitive Ion Channel TMEM63A Result in Transient Hypomyelination during Infancy
Yan, Helman, Murthy et al
Am J Hum Genet (2019) 105 (5), 996-1004 DOI: 10.1016/j.ajhg.2019.09.011
Yan, Helman, Murthy et al
Am J Hum Genet (2019) 105 (5), 996-1004 DOI: 10.1016/j.ajhg.2019.09.011
Cited by 99
Heterozygous variants in the mechanosensitive ion channel TMEM63A result in transient hypomyelination during infancy
Yan, Helman, Murthy et al
Am. J. Hum. Genet. (2019) 105 (5), 996-1004 DOI: 10.1016/j.ajhg.2019.09.011
Yan, Helman, Murthy et al
Am. J. Hum. Genet. (2019) 105 (5), 996-1004 DOI: 10.1016/j.ajhg.2019.09.011
AI Summary: OriGene’s TMEM63A cDNA clone (Cat No.: RC206992) was used as the template for introducing patient-specific variants, enabling functional analysis of mutant TMEM63A channels in the study.
Snippet: We introduced the variants seen in these individuals by site directed mutagenesis 173 into TMEM63A clones purchased from Origene (NM 014698, Cat No.: RC206992). 174 Wild-type and mutant constructs were sub-cloned into an IRES mCherry vector and 175 transfected into PIEZO1-knockout Human Embryonic Kidney 293T cells. Using a 176 previously described cell-based stretch-activated channel activity assay [20, 22], we 177 characterised the impact of each of the three variants on TMEM63A channel activity 178 (Figure 4)
OSCA/TMEM63 are an Evolutionarily Conserved Family of Mechanically Activated Ion Channels
Murthy, Dubin, Whitwam et al
Elife (2018) 7 DOI: 10.7554/eLife.41844
Murthy, Dubin, Whitwam et al
Elife (2018) 7 DOI: 10.7554/eLife.41844
AI Summary: In this study, OriGene products—including the pCMV6-Entry vector and Myc-DDK-tagged TMEM63A, TMEM63B, and TMEM63C cDNA clones—were used to express and analyze the function of TMEM63 family proteins in mechanosensitivity assays.
OSCA/TMEM63 are an Evolutionarily Conserved Family of Mechanically Activated Ion Channels
Murthy, Dubin, Whitwam et al
bioRxiv (2018)
Murthy, Dubin, Whitwam et al
bioRxiv (2018)
Showing 1-4 of 4 papers.
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