HHIP (NM_022475) Human Tagged ORF Clone
$589.00
$641.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | HHIP |
| Synonyms | HIP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_022475 |
| ORF Size | 2100 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_022475.3 |
| RefSeq Size | 3555 bp |
| RefSeq ORF | 2103 bp |
| Locus ID | 64399 |
| UniProt ID | Q96QV1 |
| Cytogenetics | 4q31.21 |
| Domains | EGF |
| Protein Families | Secreted Protein |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer |
| MW | 78.9 kDa |
| Summary | This gene encodes a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height.provided by RefSeq, Feb 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_022475" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206868L1 | Lenti ORF clone of Human hedgehog interacting protein (HHIP), Myc-DDK-tagged | 10 ug |
$941.00
|
|
| RC206868L2 | Lenti ORF clone of Human hedgehog interacting protein (HHIP), mGFP tagged | 10 ug |
$941.00
|
|
| RC206868L3 | Lenti ORF clone of Human hedgehog interacting protein (HHIP), Myc-DDK-tagged | 10 ug |
$941.00
|
|
| RC206868L4 | Lenti ORF clone of Human hedgehog interacting protein (HHIP), mGFP tagged | 10 ug |
$941.00
|
|
| RG206868 | HHIP (tGFP-tagged) - Human hedgehog interacting protein (HHIP) | 10 ug |
$789.00
$841.00
|
|
| SC120676 | HHIP (untagged)-Human hedgehog interacting protein (HHIP) | 10 ug |
$706.00
|
|
Citations
Downregulation of hedgehog-interacting protein (HHIP) contributes to hexavalent chromium-induced malignant transformation of human bronchial epithelial cells
Li, Zhang, Murphy et al
Carcinogenesis (2020)
Li, Zhang, Murphy et al
Carcinogenesis (2020)
Cited by 21
AI Summary: OriGene’s pCMV6-HHIP plasmid (RC206868) was used to ectopically express Myc-tagged HHIP in Cr(VI)-transformed cells, enabling the study of HHIP’s role in suppressing hedgehog signaling and malignant cell behaviors.
Snippet: Cr(VI)-transformed cells stably expressing Myc-tagged HHIP were established by transfecting pCMV6-HHIP (Origene, RC206868) using lipofectamine LTX reagent (Thermo Fisher Scientific, 15338030) and selected with 800ng/ml G418 (Thermo Fisher Scientific, 10131035;- Cr(VI)-transformed cells stably expressing Myc-tagged HHIP were established by transfecting pCMV6-HHIP (Origene, RC206868) using lipofectamine LTX reagent (Thermo Fisher Scientific, 15338030) and selected with 800ng/ml G418 (Thermo Fisher Scientific, 10131035
Hypoxia triggers hedgehog-mediated tumor-stromal interactions in pancreatic cancer
Spivak-Kroizman, Hostetter, Posner et al
Cancer Res. (2013) 73 (11), 3235-47 DOI: 10.1158/0008-5472.CAN-11-1433
Spivak-Kroizman, Hostetter, Posner et al
Cancer Res. (2013) 73 (11), 3235-47 DOI: 10.1158/0008-5472.CAN-11-1433
Showing 1-2 of 2 papers.
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