PRUNE (PRUNE1) (NM_021222) Human Tagged ORF Clone

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SKU
RC206865
PRUNE (Myc-DDK-tagged)-Human prune homolog (Drosophila) (PRUNE)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRUNE
Synonyms DRES-17; DRES17; H-PRUNE; HTCD37; NMIHBA; PRUNE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206865 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGACTACCTGCAGGGTTGTCGAGCTGCTCTGCAGGAGTCCCGACCTCTACATGTTGTGCTGGGAA
ATGAAGCCTGTGATTTGGACTCCACAGTGTCTGCTCTTGCCCTGGCTTTTTACCTAGCAAAGACAACTGA
GGCTGAGGAAGTCTTTGTGCCAGTTTTAAATATAAAACGTTCTGAACTACCTCTGCGAGGTGACATTGTC
TTCTTTCTTCAGAAGGTTCATATTCCAGAGAGTATCTTGATTTTTCGGGATGAGATTGACCTCCATGCAT
TATACCAGGCTGGCCAACTCACCCTCATCCTTGTCGACCATCATATCTTATCCAAAAGTGACACAGCCCT
AGAGGAGGCAGTAGCAGAGGTGCTAGACCATCGACCCATCGAGCCGAAACACTGCCCTCCCTGCCATGTT
TCAGTTGAGCTGGTGGGGTCCTGTGCTACCCTGGTGACCGAGAGAATCCTGCAGGGGGCACCAGAGATCT
TGGACAGGCAAACTGCAGCCCTTCTGCATGGAACCATCATCCTGGACTGTGTCAACATGGACCTTAAAAT
TGGAAAGGCAACCCCAAAGGACAGCAAATATGTGGAGAAACTAGAGGCCCTTTTCCCAGACCTACCCAAG
AGAAATGATATATTTGATTCCCTACAAAAGGCAAAGTTTGATGTATCAGGACTGACCACTGAGCAGATGC
TGAGAAAAGACCAGAAGACTATCTATAGACAAGGCGTCAAGGTGGCCATTAGTGCAATATATATGGATTT
GGAGGCCTTTCTGCAGAGGTCTAACCTCCTTGCAGATCTCCATGCTTTCTGCCAGGCTCACAGCTATGAT
GTCCTGGTTGCCATGACTATCTTTTTCAACACTCACAATGAGCCAGTGCGGCAGTTGGCTATTTTCTGTC
CCCATGTGGCACTCCAAACAACGATCTGTGAAGTCCTGGAACGCTCCCACTCTCCACCCCTGAAGCTGAC
CCCTGCCTCAAGTACCCACCCTAACCTCCATGCCTATCTTCAAGGCAACACCCAGGTCTCTCGAAAGAAA
CTTCTGCCCCTGCTCCAGGAAGCCCTGTCAGCATATTTTGACTCCATGAAGATCCCTTCAGGACAGCCTG
AGACAGCAGATGTGTCCAGGGAGCAAGTGGACAAGGAATTGGACAGGGCAAGTAACTCCCTGATTTCTGG
ACTGAGTCAAGATGAGGAGGACCCTCCGCTGCCCCCGACGCCCATGAACAGCTTGGTGGATGAGTGCCCT
CTAGATCAGGGGCTGCCTAAACTCTCTGCTGAGGCCGTCTTCGAGAAGTGCAGTCAGATCTCACTGTCAC
AGTCTACCACAGCCTCCCTGTCCAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206865 protein sequence
Red=Cloning site Green=Tags(s)

MEDYLQGCRAALQESRPLHVVLGNEACDLDSTVSALALAFYLAKTTEAEEVFVPVLNIKRSELPLRGDIV
FFLQKVHIPESILIFRDEIDLHALYQAGQLTLILVDHHILSKSDTALEEAVAEVLDHRPIEPKHCPPCHV
SVELVGSCATLVTERILQGAPEILDRQTAALLHGTIILDCVNMDLKIGKATPKDSKYVEKLEALFPDLPK
RNDIFDSLQKAKFDVSGLTTEQMLRKDQKTIYRQGVKVAISAIYMDLEAFLQRSNLLADLHAFCQAHSYD
VLVAMTIFFNTHNEPVRQLAIFCPHVALQTTICEVLERSHSPPLKLTPASSTHPNLHAYLQGNTQVSRKK
LLPLLQEALSAYFDSMKIPSGQPETADVSREQVDKELDRASNSLISGLSQDEEDPPLPPTPMNSLVDECP
LDQGLPKLSAEAVFEKCSQISLSQSTTASLSKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021222
ORF Size 1359 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021222.1, NP_067045.1
RefSeq Size 2995 bp
RefSeq ORF 1362 bp
Locus ID 58497
UniProt ID Q86TP1
Cytogenetics 1q21.3
Domains DHH, DHHA2
Protein Pathways Purine metabolism
MW 50.2 kDa
Summary This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2014
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Documents
Resources
"NM_021222" in other vectors (6)
SKU Description Size Price
RC206865L1 Lenti ORF clone of Human prune homolog (Drosophila) (PRUNE), Myc-DDK-tagged 10 ug
$757.00
RC206865L2 Lenti ORF clone of Human prune homolog (Drosophila) (PRUNE), mGFP tagged 10 ug
$757.00
RC206865L3 Lenti ORF clone of Human prune homolog (Drosophila) (PRUNE), Myc-DDK-tagged 10 ug
$757.00
RC206865L4 Lenti ORF clone of Human prune homolog (Drosophila) (PRUNE), mGFP tagged 10 ug
$757.00
RG206865 PRUNE (tGFP-tagged) - Human prune homolog (Drosophila) (PRUNE) 10 ug
$489.00 $657.00
SC319558 PRUNE (untagged)-Human prune homolog (Drosophila) (PRUNE) 10 ug
$457.00

Plasmid Map for RC206865

Download sequence for

Cloning scheme for RC206865

Circular map for RC206865

file-alt Citations

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