PDGF beta (PDGFB) (NM_002608) Human Tagged ORF Clone
SKU
RC206587
PDGFB (Myc-DDK-tagged)-Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PDGF beta |
| Synonyms | c-sis; IBGC5; PDGF-2; PDGF2; SIS; SSV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002608 |
| ORF Size | 723 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002608.4 |
| RefSeq Size | 3393 bp |
| RefSeq ORF | 726 bp |
| Locus ID | 5155 |
| UniProt ID | P01127 |
| Cytogenetics | 22q13.1 |
| Domains | PDGF, PDGF_N |
| Protein Families | Druggable Genome |
| Protein Pathways | Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma |
| MW | 27.3 kDa |
| Summary | This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002608" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206587L1 | Lenti ORF clone of Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC206587L2 | Lenti ORF clone of Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RC206587L3 | Lenti ORF clone of Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC206587L4 | Lenti ORF clone of Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG206587 | PDGFB (tGFP-tagged) - Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC111665 | PDGFB (untagged)-Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1 | 10 ug |
$450.00
|
|
| SC324287 | PDGFB (untagged)-Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1 | 10 ug |
$450.00
|
|
Citations
Analysis of gene expression and functional characterization of XPR1: a pathogenic gene for primary familial brain calcification
Yao, Zhao, Wang et al
Cell Tissue Res. (2017)
Yao, Zhao, Wang et al
Cell Tissue Res. (2017)
Effect of sustained PDGF nonviral gene delivery on repair of tooth-supporting bone defects
Plonka, Khorsand, Yu et al
Gene Ther. (2017) 24 (1), 31-39 DOI: 10.1038/gt.2016.73
Plonka, Khorsand, Yu et al
Gene Ther. (2017) 24 (1), 31-39 DOI: 10.1038/gt.2016.73
AI Summary: OriGene Technologies supplied the pPDGF plasmid DNA used in this study to enable sustained PDGF gene delivery for evaluating its effects on periodontal bone defect repair.
Snippet: Plasmid DNA (4.9 Kb) encoding PDGF protein (pPDGF) driven by a CMV promoter/enhancer was obtained from Origene Technologies, Inc. (Rockville, MD)
Integration of growth factor gene delivery with collagen-triggered wound repair cascades using collagen-mimetic peptides
Urello, Kiick, Sullivan
Bioeng Transl Med (2016) 1 (2), 207-219 DOI: 10.1002/btm2.10037
Urello, Kiick, Sullivan
Bioeng Transl Med (2016) 1 (2), 207-219 DOI: 10.1002/btm2.10037
AI Summary: OriGene’s pCMV-PDGF-BB plasmid was used in this study to enable gene delivery of platelet-derived growth factor-BB, facilitating analysis of its effects on wound healing processes within collagen-based biomaterials.
Snippet: Type I bovine collagen was purchased from Advanced BioMatrix (San Diego, CA) and pCMV‐PDGF‐BB plasmid was purchased from Origene Technologies, Inc. (Rockville, MD)
A Pilot Study Evaluating Combinatorial and Simultaneous Delivery of Polyethylenimine-Plasmid DNA Complexes Encoding for VEGF and PDGF for Bone Regeneration in Calvarial Bone Defects
D'Mello, Elangovan, Hong et al
Curr Pharm Biotechnol (2015) 16 (7), 655-60
D'Mello, Elangovan, Hong et al
Curr Pharm Biotechnol (2015) 16 (7), 655-60
AI Summary: In this study, OriGene products—specifically the pPDGF-B and pVEGF plasmid DNAs—were used to create gene delivery complexes that enabled the investigation of bone regeneration in rat calvarial defects.
Snippet: The transgene nuclear delivery potential of the PEI-pPDGF- B (~ 4.9 Kb pDNA, Origene Technologies, Inc., Rockville, MD) and PEI-pVEGF (~ 4.9 Kb pDNA, Origene Technolo- gies, Inc.) complexes prepared at N/P ratio of 10 and the subsequent expression of PDGF ... Cite SaveSaving
Natural polymer based gene activated matrices for bone regeneration
D'mello
Thesis (2015)
D'mello
Thesis (2015)
Snippet: Plasmid DNA (4.9 Kb) encoding PDGF-B protein (pPDGF-B) driven by CMV promoter/enhancer was obtained from Origene Technologies, Inc. (Rockville, MD)
Showing 1-5 of 5 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
