GNA14 (NM_004297) Human Tagged ORF Clone

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SKU
RC206547
GNA14 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha 14 (GNA14)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GNA14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206547 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCTGCTGCTGCCTGTCCGCGGAGGAGAAGGAGTCGCAGCGCATCAGCGCGGAGATCGAGCGAC
AGCTTCGTCGGGACAAGAAGGACGCGCGCCGTGAGCTTAAGCTGCTGCTGCTGGGAACTGGTGAAAGTGG
GAAAAGCACCTTTATCAAGCAGATGAGAATTATCCATGGGTCTGGTTACAGCGACGAAGACAGAAAGGGG
TTCACGAAGCTGGTTTACCAAAACATATTCACCGCCATGCAAGCCATGATCAGAGCGATGGACACGCTAA
GGATACAGTATGTGTGTGAACAGAATAAGGAAAATGCCCAGATAATCAGAGAAGTGGAAGTGGACAAGGT
CTCCATGCTCTCCAGGGAGCAGGTGGAGGCCATCAAGCAGCTCTGGCAAGATCCAGGCATCCAGGAGTGT
TACGACAGGAGGAGGGAGTACCAGCTGTCGGACTCTGCCAAATATTACCTGACTGACATTGACCGCATCG
CCACACCATCATTCGTGCCTACCCAACAAGATGTGCTTCGCGTCCGAGTGCCCACCACCGGCATCATTGA
GTATCCATTTGACTTGGAAAACATCATCTTTCGGATGGTGGATGTTGGTGGCCAACGATCGGAAAGACGG
AAGTGGATTCACTGCTTTGAGAGTGTCACCTCCATTATTTTCTTGGTTGCTCTGAGTGAATATGACCAGG
TCCTGGCTGAGTGTGACAACGAGAATCGCATGGAAGAGAGCAAAGCCTTATTTAAAACCATCATCACCTA
CCCCTGGTTTCTGAATTCGTCTGTGATTTTATTCTTGAACAAGAAGGATCTTTTGGAAGAGAAAATCATG
TACTCTCATCTAATTAGCTATTTCCCAGAATACACAGGACCGAAACAGGATGTCAGAGCTGCCAGAGACT
TTATCCTGAAGCTTTACCAAGATCAGAATCCTGACAAAGAGAAAGTCATCTACTCTCACTTCACATGTGC
TACAGATACAGACAATATTCGCTTTGTGTTTGCTGCTGTCAAAGACACAATTCTACAGCTAAACCTAAGG
GAATTCAACCTTGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206547 protein sequence
Red=Cloning site Green=Tags(s)

MAGCCCLSAEEKESQRISAEIERQLRRDKKDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDRKG
FTKLVYQNIFTAMQAMIRAMDTLRIQYVCEQNKENAQIIREVEVDKVSMLSREQVEAIKQLWQDPGIQEC
YDRRREYQLSDSAKYYLTDIDRIATPSFVPTQQDVLRVRVPTTGIIEYPFDLENIIFRMVDVGGQRSERR
KWIHCFESVTSIIFLVALSEYDQVLAECDNENRMEESKALFKTIITYPWFLNSSVILFLNKKDLLEEKIM
YSHLISYFPEYTGPKQDVRAARDFILKLYQDQNPDKEKVIYSHFTCATDTDNIRFVFAAVKDTILQLNLR
EFNLV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004297
ORF Size 1065 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004297.4
RefSeq Size 2492 bp
RefSeq ORF 1068 bp
Locus ID 9630
UniProt ID O95837
Cytogenetics 9q21.2
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway
MW 41.6 kDa
Summary This gene encodes a member of the guanine nucleotide-binding, or G protein family. G proteins are heterotrimers consisting of alpha, beta and gamma subunits. The encoded protein is a member of the alpha family of G proteins, more specifically the alpha q subfamily of G proteins. The encoded protein may play a role in pertussis-toxin resistant activation of phospholipase C-beta and its downstream effectors.provided by RefSeq, Feb 2009
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Documents
Resources
"NM_004297" in other vectors (4)
SKU Description Size Price
RC206547L3 Lenti ORF clone of Human guanine nucleotide binding protein (G protein), alpha 14 (GNA14), Myc-DDK-tagged 10 ug
$757.00
RC206547L4 Lenti ORF clone of Human guanine nucleotide binding protein (G protein), alpha 14 (GNA14), mGFP tagged 10 ug
$757.00
RG206547 GNA14 (tGFP-tagged) - Human guanine nucleotide binding protein (G protein), alpha 14 (GNA14) 10 ug
$657.00
SC122675 GNA14 (untagged)-Human guanine nucleotide binding protein (G protein), alpha 14 (GNA14) 10 ug
$457.00

Plasmid Map for RC206547

Download sequence for

Cloning scheme for RC206547

Circular map for RC206547

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