GPR182 (NM_007264) Human Tagged ORF Clone

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SKU
RC206505
GPR182 (Myc-DDK-tagged)-Human G protein-coupled receptor 182 (GPR182)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPR182
Synonyms 7TMR; ADMR; AM-R; AMR; G10D; gamrh; hrhAMR; L1-R
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206505 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGTGAAACCCAGCTGGGGGCCTGGCCCCTCGGAGGGGGTCACCGCAGTGCCTACCAGTGACCTTG
GAGAGATCCACAACTGGACCGAGCTGCTTGACCTCTTCAACCACACTTTGTCTGAGTGCCACGTGGAGCT
CAGCCAGAGCACCAAGCGCGTGGTCCTCTTTGCCCTCTACCTGGCCATGTTTGTGGTTGGGCTGGTGGAG
AACCTCCTGGTGATATGCGTCAACTGGCGCGGCTCAGGCCGGGCAGGGCTGATGAACCTCTACATCCTCA
ACATGGCCATCGCGGACCTGGGCATTGTCCTGTCTCTGCCCGTGTGGATGCTGGAGGTCACGCTGGACTA
CACCTGGCTCTGGGGCAGCTTCTCCTGCCGCTTCACTCACTACTTCTACTTTGTCAACATGTATAGCAGC
ATCTTCTTCCTGGTGTGCCTCAGTGTCGACCGCTATGTCACCCTCACCAGCGCCTCCCCCTCCTGGCAGC
GTTACCAGCACCGAGTGCGGCGGGCCATGTGTGCAGGCATCTGGGTCCTCTCGGCCATCATCCCGCTGCC
TGAGGTGGTCCACATCCAGCTGGTGGAGGGCCCTGAGCCCATGTGCCTCTTCATGGCACCTTTTGAAACG
TACAGCACCTGGGCCCTGGCGGTGGCCCTGTCCACCACCATCCTGGGCTTCCTGCTGCCCTTCCCTCTCA
TCACAGTCTTCAATGTGCTGACAGCCTGCCGGCTGCGGCAGCCAGGACAACCCAAGAGCCGGCGCCACTG
CCTGCTGCTGTGCGCCTACGTGGCCGTCTTTGTCATGTGCTGGCTGCCCTATCATGTGACCCTGCTGCTG
CTCACACTGCATGGGACCCACATCTCCCTCCACTGCCACCTGGTCCACCTGCTCTACTTCTTCTATGATG
TCATTGACTGCTTCTCCATGCTGCACTGTGTCATCAACCCCATCCTTTACAACTTTCTCAGCCCACACTT
CCGGGGCCGGCTCCTGAATGCTGTAGTCCATTACCTTCCTAAGGACCAGACCAAGGCGGGCACATGCGCC
TCCTCTTCCTCCTGTTCCACCCAGCATTCCATCATCATCACCAAGGGTGATAGCCAGCCTGCTGCAGCAG
CCCCCCACCCTGAGCCAAGCCTGAGCTTTCAGGCACACCATTTGCTTCCAAATACTTCCCCCATCTCTCC
CACTCAGCCTCTTACACCCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206505 protein sequence
Red=Cloning site Green=Tags(s)

MSVKPSWGPGPSEGVTAVPTSDLGEIHNWTELLDLFNHTLSECHVELSQSTKRVVLFALYLAMFVVGLVE
NLLVICVNWRGSGRAGLMNLYILNMAIADLGIVLSLPVWMLEVTLDYTWLWGSFSCRFTHYFYFVNMYSS
IFFLVCLSVDRYVTLTSASPSWQRYQHRVRRAMCAGIWVLSAIIPLPEVVHIQLVEGPEPMCLFMAPFET
YSTWALAVALSTTILGFLLPFPLITVFNVLTACRLRQPGQPKSRRHCLLLCAYVAVFVMCWLPYHVTLLL
LTLHGTHISLHCHLVHLLYFFYDVIDCFSMLHCVINPILYNFLSPHFRGRLLNAVVHYLPKDQTKAGTCA
SSSSCSTQHSIIITKGDSQPAAAAPHPEPSLSFQAHHLLPNTSPISPTQPLTPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007264
ORF Size 1212 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007264.4
RefSeq Size 1620 bp
RefSeq ORF 1215 bp
Locus ID 11318
UniProt ID O15218
Cytogenetics 12q13.3
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
MW 45.3 kDa
Summary Adrenomedullin is a potent vasodilator peptide that exerts major effects on cardiovascular function. This gene encodes a seven-transmembrane protein that belongs to the family 1 of G-protein coupled receptors. Studies of the rat counterpart suggest that the encoded protein may function as a receptor for adrenomedullin. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_007264" in other vectors (6)
SKU Description Size Price
RC206505L1 Lenti ORF clone of Human G protein-coupled receptor 182 (GPR182), Myc-DDK-tagged 10 ug
$757.00
RC206505L2 Lenti ORF clone of Human G protein-coupled receptor 182 (GPR182), mGFP tagged 10 ug
$757.00
RC206505L3 Lenti ORF clone of Human G protein-coupled receptor 182 (GPR182), Myc-DDK-tagged 10 ug
$757.00
RC206505L4 Lenti ORF clone of Human G protein-coupled receptor 182 (GPR182), mGFP tagged 10 ug
$757.00
RG206505 GPR182 (tGFP-tagged) - Human G protein-coupled receptor 182 (GPR182) 10 ug
$489.00 $657.00
SC115624 GPR182 (untagged)-Human G protein-coupled receptor 182 (GPR182) 10 ug
$457.00

Plasmid Map for RC206505

Download sequence for

Cloning scheme for RC206505

Circular map for RC206505

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