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Granzyme B (GZMB) (NM_004131) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC206495
GZMB (Myc-DDK-tagged)-Human granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
2 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Granzyme B or NM_004131"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Granzyme B
Synonyms C11; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206495 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAACCAATCCTGCTTCTGCTGGCCTTCCTCCTGCTGCCCAGGGCAGATGCAGGGGAGATCATCGGGG
GACATGAGGCCAAGCCCCACTCCCGCCCCTACATGGCTTATCTTATGATCTGGGATCAGAAGTCTCTGAA
GAGGTGCGGTGGCTTCCTGATACAAGACGACTTCGTGCTGACAGCTGCTCACTGTTGGGGAAGCTCCATA
AATGTCACCTTGGGGGCCCACAATATCAAAGAACAGGAGCCGACCCAGCAGTTTATCCCTGTGAAAAGAC
CCATCCCCCATCCAGCCTATAATCCTAAGAACTTCTCCAACGACATCATGCTACTGCAGCTGGAGAGAAA
GGCCAAGCGGACCAGAGCTGTGCAGCCCCTCAGGCTACCTAGCAACAAGGCCCAGGTGAAGCCAGGGCAG
ACATGCAGTGTGGCCGGCTGGGGGCAGACGGCCCCCCTGGGAAAACACTCACACACACTACAAGAGGTGA
AGATGACAGTGCAGGAAGATCGAAAGTGCGAATCTGACTTACGCCATTATTACGACAGTACCATTGAGTT
GTGCGTGGGGGACCCAGAGATTAAAAAGACTTCCTTTAAGGGGGACTCTGGAGGCCCTCTTGTGTGTAAC
AAGGTGGCCCAGGGCATTGTCTCCTATGGACGAAACAATGGCATGCCTCCACGAGCCTGCACCAAAGTCT
CAAGCTTTGTACACTGGATAAAGAAAACCATGAAACGCCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206495 protein sequence
Red=Cloning site Green=Tags(s)

MQPILLLLAFLLLPRADAGEIIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIQDDFVLTAAHCWGSSI
NVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQ
TCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCN
KVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004131
ORF Size 741 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004131.6
RefSeq Size 941 bp
RefSeq ORF 744 bp
Locus ID 3002
UniProt ID P10144
Cytogenetics 14q12
Domains Tryp_SPc
Protein Families Druggable Genome, Protease
Protein Pathways Allograft rejection, Autoimmune thyroid disease, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus
MW 27.7 kDa
Summary This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. provided by RefSeq, Sep 2016
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"NM_004131" in other vectors (6)
SKU Description Size Price
RC206495L1 Lenti ORF clone of Human granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB), Myc-DDK-tagged 10 ug
$600.00
RC206495L2 Lenti ORF clone of Human granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB), mGFP tagged 10 ug
$600.00
RC206495L3 Lenti ORF clone of Human granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB), Myc-DDK-tagged 10 ug
$600.00
RC206495L4 Lenti ORF clone of Human granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB), mGFP tagged 10 ug
$600.00
RG206495 GZMB (tGFP-tagged) - Human granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB) 10 ug
$489.00 $500.00
SC321693 GZMB (untagged)-Human granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) (GZMB) 10 ug
$300.00

Plasmid Map for RC206495

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206495
Circular map for RC206495

file-alt Citations

A Microfluidic Platform for Single Cell Fluorometric Granzyme B Profiling
Briones, Espulgar, Koyama et al
Theranostics (2020) 10 (1), 123-132 DOI: 10.7150/thno.37728
Cited by 28
AI Summary: In this study, OriGene’s anti-human Granzyme B antibody (Cat# TA336463) and GrB-transduced Jurkat cells (Cat# RC220838L2) were used to validate and benchmark the single-cell Granzyme B activity measurements on the microfluidic platform, supporting the assessment of cytotoxic responses in immune cell populations.
FigureFigureFigure
A Microfluidic Platform for Single Cell Fluorometric Granzyme B Profiling
Briones, Espulgar, Koyama et al
Theranostics (2019) 10 (1), 123-132 DOI: 10.7150/thno.37728
AI Summary: OriGene’s Granzyme B plasmid (RC206495) was used as the source of Granzyme B cDNA for generating lentiviral constructs to transduce Jurkat cells, enabling the study of single-cell Granzyme B activity in the microfluidic platform described in the paper.
Snippet: Briefly, a CDS of GrB was cloned from a commercially available GrB plasmid (Origene, RC206495) and inserted into lentiviral vector CSII-EF-MCS-IRES2-Venus (RIKEN, Dr. Miyoshi's lab). Jurkat cells were transduced with lentiviral GrB plasmids by spinfection;;- Briefly, a CDS of GrB was cloned from a commercially available GrB plasmid (Origene, RC206495) and inserted into lentiviral vector CSII-EF-MCS- IRES2-Venus (RIKEN, Dr. Miyoshi's lab). …
Showing 1-2 of 2 papers.
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