Myozenin 1 (MYOZ1) (NM_021245) Human Tagged ORF Clone

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SKU
RC206448
MYOZ1 (Myc-DDK-tagged)-Human myozenin 1 (MYOZ1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Myozenin 1
Synonyms CS-2; FATZ; MYOZ
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206448 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCTCTCAGGAACCCCGGCCCCTAATAAGAAGAGGAAATCCAGCAAGCTGATCATGGAACTCACTG
GAGGTGGACAGGAGAGCTCAGGCTTGAACCTGGGCAAAAAGATCAGTGTCCCAAGGGATGTGATGTTGGA
GGAACTGTCGCTGCTTACCAACCGGGGCTCCAAGATGTTCAAACTGCGGCAGATGAGGGTGGAGAAGTTT
ATTTATGAGAACCACCCTGATGTTTTCTCTGACAGCTCAATGGATCACTTCCAGAAGTTCCTTCCAACAG
TGGGGGGACAGCTGGGCACAGCTGGTCAGGGATTCTCATACAGCAAGAGCAACGGCAGAGGCGGCAGCCA
GGCAGGGGGCAGTGGCTCTGCCGGACAGTATGGCTCTGATCAGCAGCACCATCTGGGCTCTGGGTCTGGA
GCTGGGGGTACAGGTGGTCCCGCGGGCCAGGCTGGCAGAGGAGGAGCTGCTGGCACAGCAGGGGTTGGTG
AGACAGGATCAGGAGACCAGGCAGGCGGAGAAGGAAAACATATCACTGTGTTCAAGACCTATATTTCCCC
ATGGGAGCGAGCCATGGGGGTTGACCCCCAGCAAAAAATGGAACTTGGCATTGACCTGCTGGCCTATGGG
GCCAAAGCTGAACTTCCCAAATATAAGTCCTTCAACAGGACGGCAATGCCCTATGGTGGATATGAGAAGG
CCTCCAAACGCATGACCTTCCAGATGCCCAAGTTTGACCTGGGGCCCTTGCTGAGTGAACCCCTGGTCCT
CTACAACCAAAACCTCTCCAACAGGCCTTCTTTCAATCGAACCCCTATTCCCTGGCTGAGCTCTGGGGAG
CCTGTAGACTACAACGTGGATATTGGCATCCCCTTGGATGGAGAAACAGAGGAGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206448 protein sequence
Red=Cloning site Green=Tags(s)

MPLSGTPAPNKKRKSSKLIMELTGGGQESSGLNLGKKISVPRDVMLEELSLLTNRGSKMFKLRQMRVEKF
IYENHPDVFSDSSMDHFQKFLPTVGGQLGTAGQGFSYSKSNGRGGSQAGGSGSAGQYGSDQQHHLGSGSG
AGGTGGPAGQAGRGGAAGTAGVGETGSGDQAGGEGKHITVFKTYISPWERAMGVDPQQKMELGIDLLAYG
AKAELPKYKSFNRTAMPYGGYEKASKRMTFQMPKFDLGPLLSEPLVLYNQNLSNRPSFNRTPIPWLSSGE
PVDYNVDIGIPLDGETEEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021245
ORF Size 897 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021245.4
RefSeq Size 1583 bp
RefSeq ORF 900 bp
Locus ID 58529
UniProt ID Q9NP98
Cytogenetics 10q22.2
MW 31.7 kDa
Summary The protein encoded by this gene is primarily expressed in the skeletal muscle, and belongs to the myozenin family. Members of this family function as calcineurin-interacting proteins that help tether calcineurin to the sarcomere of cardiac and skeletal muscle. They play an important role in modulation of calcineurin signaling. provided by RefSeq, Apr 2012
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Documents
Resources
"NM_021245" in other vectors (5)
SKU Description Size Price
RC206448L3 Lenti ORF clone of Human myozenin 1 (MYOZ1), Myc-DDK-tagged 10 ug
$600.00
RC206448L4 Lenti ORF clone of Human myozenin 1 (MYOZ1), mGFP tagged 10 ug
$600.00
RG206448 MYOZ1 (tGFP-tagged) - Human myozenin 1 (MYOZ1) 10 ug
$489.00 $500.00
SC112943 MYOZ1 (untagged)-Human myozenin 1 (MYOZ1) 10 ug
$300.00
SC322546 MYOZ1 (untagged)-Human myozenin 1 (MYOZ1) 10 ug
$300.00

Plasmid Map for RC206448

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Cloning scheme for RC206448

Circular map for RC206448

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