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FBXL16 (NM_153350) Human Tagged ORF Clone

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SKU
RC206362
FBXL16 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 16 (FBXL16)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXL16
Synonyms C16orf22; c380A1.1; Fbl16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206362 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAGCCCGGGCATCGACGGCGACCCCAAGCCTCCATGCTTGCCTCGAAACGGTCTGGTGAAGCTGC
CGGGCCAGCCCAACGGCCTGGGTGCGGCCAGCATCACCAAGGGCACGCCAGCCACCAAGAACCGCCCCTG
CCAGCCACCACCCCCACCCACCCTCCCACCACCAAGCCTGGCTGCTCCACTGTCCCGGGCTGCCCTGGCT
GGGGGCCCGTGCACCCCGGCAGGTGGACCAGCCTCAGCCTTGGCACCTGGGCACCCAGCGGAGCGGCCGC
CGCTGGCCACGGACGAGAAGATCCTCAATGGGCTCTTCTGGTATTTCTCGGCCTGCGAGAAGTGTGTGCT
GGCCCAGGTGTGCAAGGCCTGGCGGCGCGTGCTGTACCAGCCCAAGTTCTGGGCAGGCCTCACGCCGGTG
CTGCATGCCAAGGAGCTCTACAACGTGCTGCCTGGTGGCGAGAAGGAGTTCGTGAACCTGCAGGGTTTTG
CCGCCAGAGGCTTCGAGGGCTTCTGCCTGGTTGGCGTCTCCGACCTGGACATCTGTGAGTTCATTGACAA
CTATGCGCTCTCCAAGAAGGGTGTCAAAGCCATGAGCCTCAAGCGCTCCACCATCACGGACGCAGGCCTC
GAGGTTATGCTTGAACAGATGCAGGGCGTGGTGCGTCTGGAGCTGTCGGGCTGCAACGACTTCACCGAGG
CCGGGCTGTGGTCCAGCCTGAGCGCGCGCATCACCTCGCTGAGCGTGAGTGACTGCATCAACGTGGCCGA
CGACGCCATCGCGGCCATCTCGCAGCTGCTGCCCAACCTGGCGGAGCTGAGCCTGCAGGCCTACCACGTG
ACGGACACGGCGCTGGCCTACTTCACGGCGCGCCAGGGCCACAGCACGCACACGCTGCGCCTGCTCTCCT
GCTGGGAGATCACCAACCACGGCGTGGTCAACGTGGTGCACAGCCTGCCCAACCTCACCGCGCTCAGCCT
CTCGGGCTGCTCCAAGGTCACCGACGACGGCGTGGAGCTCGTGGCCGAGAACCTGCGCAAGCTGCGCAGC
CTTGACCTCTCGTGGTGCCCACGCATCACCGACATGGCGCTGGAGTACGTGGCCTGCGACCTGCACCGCC
TAGAGGAGCTCGTGCTCGACAGGTGTGTACGCATCACGGACACTGGCCTCAGCTATCTGTCCACCATGTC
GTCCCTCCGCAGCCTCTACCTGCGATGGTGCTGCCAGGTGCAAGACTTCGGGCTGAAGCACCTCCTGGCC
CTGGGGAGTTTGCGCCTCCTGTCTCCGGCAGGCTGCCCGCTGCTCACCACCACCGGGCTGTCGGGCCTGG
TGCAGCTGCAGGAGCTGGAGGAGCTGGAGCTGACCAACTGCCCCGGGGCCACCCCCGAGCTCTTCAAGTA
TTTCTCGCAGCACCTGCCCCGCTGCCTCGTCATTGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206362 protein sequence
Red=Cloning site Green=Tags(s)

MSSPGIDGDPKPPCLPRNGLVKLPGQPNGLGAASITKGTPATKNRPCQPPPPPTLPPPSLAAPLSRAALA
GGPCTPAGGPASALAPGHPAERPPLATDEKILNGLFWYFSACEKCVLAQVCKAWRRVLYQPKFWAGLTPV
LHAKELYNVLPGGEKEFVNLQGFAARGFEGFCLVGVSDLDICEFIDNYALSKKGVKAMSLKRSTITDAGL
EVMLEQMQGVVRLELSGCNDFTEAGLWSSLSARITSLSVSDCINVADDAIAAISQLLPNLAELSLQAYHV
TDTALAYFTARQGHSTHTLRLLSCWEITNHGVVNVVHSLPNLTALSLSGCSKVTDDGVELVAENLRKLRS
LDLSWCPRITDMALEYVACDLHRLEELVLDRCVRITDTGLSYLSTMSSLRSLYLRWCCQVQDFGLKHLLA
LGSLRLLSPAGCPLLTTTGLSGLVQLQELEELELTNCPGATPELFKYFSQHLPRCLVIE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153350
ORF Size 1437 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153350.2, NP_699181.1
RefSeq Size 3543 bp
RefSeq ORF 1440 bp
Locus ID 146330
UniProt ID Q8N461
Cytogenetics 16p13.3
Domains F-box, LRR, LRR_CC
MW 51.6 kDa
Summary Members of the F-box protein family, such as FBXL16, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 PubMed 15520277).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_153350" in other vectors (6)
SKU Description Size Price
RC206362L1 Lenti ORF clone of Human F-box and leucine-rich repeat protein 16 (FBXL16), Myc-DDK-tagged 10 ug
$757.00
RC206362L2 Lenti ORF clone of Human F-box and leucine-rich repeat protein 16 (FBXL16), mGFP tagged 10 ug
$757.00
RC206362L3 Lenti ORF clone of Human F-box and leucine-rich repeat protein 16 (FBXL16), Myc-DDK-tagged 10 ug
$757.00
RC206362L4 Lenti ORF clone of Human F-box and leucine-rich repeat protein 16 (FBXL16), mGFP tagged 10 ug
$757.00
RG206362 FBXL16 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 16 (FBXL16) 10 ug
$657.00
SC316382 FBXL16 (untagged)-Human F-box and leucine-rich repeat protein 16 (FBXL16) 10 ug
$457.00

Plasmid Map for RC206362

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206362
Circular map for RC206362

file-alt Citations

Suppression of Breast Cancer Progression by FBXL16 Via Oxygen-Independent Regulation of HIF1α Stability
Kim, Zhao, Myung et al
SSRN Journal (2021) 37 (8), 109996 DOI: 10.1016/j.celrep.2021.109996
Cited by 31
AI Summary: OriGene products, specifically the FBXL16 vector (#RC206362) and the VHL vector (#RC216151), were used in this study to investigate the roles of FBXL16 and VHL in the regulation of HIF1α stability and breast cancer progression.
Snippet: …The vectors of FBXL16 (#RC206362) and VHL 488 (#RC216151) were purchased from Origene (Rockville, MD, USA);the HIF1α vector (#18949) was 489 obtained from Addgene (Watertown, MA, USA)
Showing 1-1 of 1 papers.
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