Progestin Receptor Beta (PAQR8) (NM_133367) Human Tagged ORF Clone

  • TrueORF®
SKU
RC206303
PAQR8 (Myc-DDK-tagged)-Human progestin and adipoQ receptor family member VIII (PAQR8)
  $457.00
5 Days*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Progestin Receptor Beta
Synonyms C6orf33; LMPB1; MPRB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206303 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGACCGCCATCTTGGAGCGCCTGAGCACCCTGTCGGTCAGCGGGCAGCGGCTGCGCCGCCTGCCCA
AGATCCTGGAGGATGGGCTTCCCAAGATGCCTTGCACTGTCCCAGAAACGGATGTGCCCCAGCTCTTCCG
GGAGCCTTACATCCGCACCGGCTACCGCCCCACGGGGCACGAGTGGCGCTACTACTTCTTCAGCCTCTTT
CAGAAACACAACGAGGTGGTCAACGTCTGGACCCATTTACTGGCAGCCCTGGCCGTCCTCTTGCGATTCT
GGGCCTTTGCCGAGGCTGAGGCCTTGCCATGGGCGTCTACCCACTCCCTGCCTCTGCTCCTCTTCATCCT
GTCGTCAATCACTTACCTCACCTGCAGCCTTCTGGCCCACCTGCTGCAGTCCAAGTCAGAGCTCTCCCAC
TACACCTTCTACTTTGTGGACTATGTTGGCGTGAGCGTTTACCAATATGGCAGTGCTTTGGCTCATTTCT
TCTACAGCTCTGACCAGGCCTGGTATGACCGGTTCTGGCTTTTCTTCTTGCCAGCAGCTGCCTTCTGTGG
CTGGTTATCTTGTGCTGGCTGTTGCTATGCCAAATATTGTTACCGGAGGCCTTATCCAGTCATGAGGAAG
ATCTGTCAAGTGGTGCCAGCAGGTCTGGCTTTTATCCTAGACATCAGCCCTGTGGCACACCGTGTGGCGC
TCTGTCACCTGGCTGGCTGCCAGGAGCAAGCAGCCTGGTACCACACCCTCCAGATCCTCTTCTTCCTGGT
TAGCGCTTATTTCTTCTCCTGCCCCGTGCCTGAGAAGTACTTCCCGGGTTCCTGTGACATCGTGGGCCAT
GGGCATCAGATCTTCCATGCATTTCTGTCCATCTGTACGCTCTCCCAGCTGGAGGCCATCCTCCTGGACT
ACCAGGGGCGGCAGGAGATCTTCCTGCAGCGCCATGGACCCCTATCTGTCCACATGGCCTGCCTCTCCTT
CTTCTTCCTGGCTGCCTGCAGTGCTGCCACCGCAGCCCTTCTGAGGCACAAAGTCAAGGCCAGACTGACC
AAGAAAGATTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206303 protein sequence
Red=Cloning site Green=Tags(s)

MTTAILERLSTLSVSGQRLRRLPKILEDGLPKMPCTVPETDVPQLFREPYIRTGYRPTGHEWRYYFFSLF
QKHNEVVNVWTHLLAALAVLLRFWAFAEAEALPWASTHSLPLLLFILSSITYLTCSLLAHLLQSKSELSH
YTFYFVDYVGVSVYQYGSALAHFFYSSDQAWYDRFWLFFLPAAAFCGWLSCAGCCYAKYCYRRPYPVMRK
ICQVVPAGLAFILDISPVAHRVALCHLAGCQEQAAWYHTLQILFFLVSAYFFSCPVPEKYFPGSCDIVGH
GHQIFHAFLSICTLSQLEAILLDYQGRQEIFLQRHGPLSVHMACLSFFFLAACSAATAALLRHKVKARLT
KKDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133367
ORF Size 1062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133367.5
RefSeq Size 4758 bp
RefSeq ORF 1065 bp
Locus ID 85315
UniProt ID Q8TEZ7, B3KXW6
Cytogenetics 6p12.2
Domains UPF0073
Protein Families Druggable Genome, Transmembrane
MW 40.4 kDa
Summary Plasma membrane progesterone (P4) receptor coupled to G proteins (PubMed:23763432). Seems to act through a G(i) mediated pathway (PubMed:23763432). May be involved in oocyte maturation (By similarity). Also binds dehydroepiandrosterone (DHEA), pregnanolone, pregnenolone and allopregnanolone (PubMed:23161870).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133367" in other vectors (4)
SKU Description Size Price
RC206303L3 Lenti ORF clone of Human progestin and adipoQ receptor family member VIII (PAQR8), Myc-DDK-tagged 10 ug
$757.00
RC206303L4 Lenti ORF clone of Human progestin and adipoQ receptor family member VIII (PAQR8), mGFP tagged 10 ug
$757.00
RG206303 PAQR8 (tGFP-tagged) - Human progestin and adipoQ receptor family member VIII (PAQR8) 10 ug
$489.00 $657.00
SC120538 PAQR8 (untagged)-Human progestin and adipoQ receptor family member VIII (PAQR8) 10 ug
$457.00

Plasmid Map for RC206303

Download sequence for

Cloning scheme for RC206303

Circular map for RC206303

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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