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SLC6A1 (NM_003042) Human Tagged ORF Clone

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SKU
RC206290
SLC6A1 (Myc-DDK-tagged)-Human solute carrier family 6 (neurotransmitter transporter, GABA), member 1 (SLC6A1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $558.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC6A1
Synonyms GABATHG; GABATR; GAT1; MAE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206290 representing NM_003042
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCAACGGCAGCAAGGTGGCCGACGGGCAGATCTCCACCGAGGTCAGCGAGGCCCCTGTGGCCA
ATGACAAGCCCAAAACCTTGGTGGTCAAGGTGCAGAAGAAGGCGGCAGACCTCCCCGACCGGGACACGTG
GAAGGGCCGCTTCGACTTCCTCATGTCCTGTGTGGGCTATGCCATCGGCCTGGGCAACGTCTGGAGGTTC
CCCTATCTCTGCGGGAAAAATGGTGGGGGAGCCTTCCTGATCCCCTATTTCCTGACACTCATCTTTGCGG
GGGTCCCACTCTTCCTGCTGGAGTGCTCCCTGGGCCAGTACACCTCCATCGGGGGGCTAGGGGTATGGAA
GCTGGCTCCTATGTTCAAGGGCGTGGGCCTTGCGGCTGCTGTGCTATCATTCTGGCTGAACATCTACTAC
ATCGTCATCATCTCCTGGGCCATTTACTACCTGTACAACTCCTTCACCACGACACTGCCGTGGAAACAGT
GCGACAACCCCTGGAACACAGACCGCTGCTTCTCCAACTACAGCATGGTCAACACTACCAACATGACCAG
CGCTGTGGTGGAGTTCTGGGAGCGCAACATGCATCAGATGACGGACGGGCTGGATAAGCCAGGTCAGATC
CGCTGGCCACTGGCCATCACGCTGGCCATCGCCTGGATCCTTGTGTATTTCTGTATCTGGAAGGGTGTTG
GCTGGACTGGAAAGGTGGTCTACTTTTCAGCCACATACCCCTACATCATGCTGATCATCCTGTTCTTCCG
TGGAGTGACGCTGCCCGGGGCCAAGGAGGGCATCCTCTTCTACATCACACCCAACTTCCGCAAGCTGTCT
GACTCCGAGGTGTGGCTGGATGCGGCAACCCAGATCTTCTTCTCATACGGGCTGGGCCTGGGGTCCCTGA
TCGCTCTCGGGAGCTACAACTCTTTCCACAACAATGTCTACAGGGACTCCATCATCGTCTGCTGCATCAA
TTCGTGCACCAGCATGTTCGCAGGATTCGTCATCTTCTCCATCGTGGGCTTCATGGCCCATGTCACGAAG
AGGTCCATTGCTGATGTGGCGGCCTCAGGCCCCGGGCTGGCGTTCCTGGCATACCCAGAGGCGGTGACCC
AGCTGCCTATCTCCCCACTCTGGGCCATCCTCTTCTTCTCCATGCTGTTGATGCTGGGCATTGACAGCCA
GTTCTGCACTGTGGAGGGCTTCATCACAGCCCTGGTGGATGAGTACCCCAGGCTCCTCCGCAACCGCAGA
GAGCTCTTCATTGCTGCTGTCTGCATCATCTCCTACCTGATCGGTCTCTCTAACATCACTCAGGGGGGTA
TTTATGTCTTCAAACTCTTTGACTACTACTCTGCCAGTGGCATGAGCCTGCTGTTCCTCGTGTTCTTTGA
ATGTGTCTCTATTTCCTGGTTTTACGGTGTCAACCGATTCTATGACAATATCCAAGAGATGGTTGGATCC
AGGCCCTGCATCTGGTGGAAACTCTGCTGGTCTTTCTTCACACCAATCATTGTGGCGGGCGTGTTCATTT
TCAGTGCTGTGCAGATGACGCAACTCACCATGGGAAACTATGTTTTCCCCAAGTGGGGCCAGGGTGTGGG
CTGGCTGATGGCTCTGTCTTCCATGGTCCTCATCCCCGGGTACATGGCCTACATGTTCCTCACCTTAAAG
GGCTCCCTGAAGCAGCGCATCCAAGTCATGGTCCAGCCCAGCGAAGACATCGTTCGCCCAGAGAATGGTC
CTGAGCAGCCCCAGGCGGGCAGCTCCACCAGCAAGGAGGCCTACATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206290 representing NM_003042
Red=Cloning site Green=Tags(s)

MATNGSKVADGQISTEVSEAPVANDKPKTLVVKVQKKAADLPDRDTWKGRFDFLMSCVGYAIGLGNVWRF
PYLCGKNGGGAFLIPYFLTLIFAGVPLFLLECSLGQYTSIGGLGVWKLAPMFKGVGLAAAVLSFWLNIYY
IVIISWAIYYLYNSFTTTLPWKQCDNPWNTDRCFSNYSMVNTTNMTSAVVEFWERNMHQMTDGLDKPGQI
RWPLAITLAIAWILVYFCIWKGVGWTGKVVYFSATYPYIMLIILFFRGVTLPGAKEGILFYITPNFRKLS
DSEVWLDAATQIFFSYGLGLGSLIALGSYNSFHNNVYRDSIIVCCINSCTSMFAGFVIFSIVGFMAHVTK
RSIADVAASGPGLAFLAYPEAVTQLPISPLWAILFFSMLLMLGIDSQFCTVEGFITALVDEYPRLLRNRR
ELFIAAVCIISYLIGLSNITQGGIYVFKLFDYYSASGMSLLFLVFFECVSISWFYGVNRFYDNIQEMVGS
RPCIWWKLCWSFFTPIIVAGVFIFSAVQMTQLTMGNYVFPKWGQGVGWLMALSSMVLIPGYMAYMFLTLK
GSLKQRIQVMVQPSEDIVRPENGPEQPQAGSSTSKEAYI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003042
ORF Size 1797 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003042.2, NP_003033.2
RefSeq Size 4493 bp
RefSeq ORF 1800 bp
Locus ID 6529
UniProt ID P30531, A0A024R2K8
Cytogenetics 3p25.3
Domains SNF
Protein Families Druggable Genome, Transmembrane
MW 66.9 kDa
Summary The protein encoded by this gene is a gamma-aminobutyric acid (GABA) transporter that localizes to the plasma membrane. The encoded protein removes GABA from the synaptic cleft, restoring it to presynaptic terminals. provided by RefSeq, Jan 2017
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"NM_003042" in other vectors (4)
SKU Description Size Price
RC206290L3 Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, GABA), member 1 (SLC6A1), Myc-DDK-tagged 10 ug
$858.00
RC206290L4 Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, GABA), member 1 (SLC6A1), mGFP tagged 10 ug
$858.00
RG206290 SLC6A1 (tGFP-tagged) - Human solute carrier family 6 (neurotransmitter transporter, GABA), member 1 (SLC6A1) 10 ug
$489.00 $758.00
SC126769 SLC6A1 (untagged)-Human solute carrier family 6 (neurotransmitter transporter, GABA), member 1 (SLC6A1) 10 ug
$558.00

Plasmid Map for RC206290

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206290
Circular map for RC206290

file-alt Citations

Silencing of SLC6A1 Enhanced Cell Apoptosis in Prostate Cancer Through a Mitochondrial Mechanism
Chen, Wang, Lin et al
Advances in Urology (2026) 2026 (1) DOI: 10.1155/aiu/7448726
AI Summary: OriGene products contributed to this study by providing the human SLC6A1 ORF clone plasmid (RC206290, pCMV6-Entry) for overexpression experiments and the matched empty vector (PS100001) as a control, enabling the investigation of SLC6A1’s role in prostate cancer cell apoptosis.
Snippet: siRNA to achieve SLC6A1 overexpression or silencing. For SLC6A1 overexpression, an OriGene human SLC6A1 ORF clone plasmid (RC206290, pCMV6-Entry) was used, and the matched empty vector pCMV6-Entry (PS100001) served as the vector control. For SLC6A1 knockdown, Stealth RNAi siRNA targeting human
Rescue of Epilepsy-Associated Mutations of the Highly Conserved Glycine Residue 443 in the Human GABA Transporter 1
Shah, Kovalchuk, Zerlotti et al
FASEB J (2025) 39 (11), e70614 DOI: 10.1096/fj.202403159RR
AI Summary: OriGene's WT hGAT-1 plasmid (cat. no. RC206290) was used as the template for generating epilepsy-associated G443 mutations in the human GABA transporter 1, enabling the study of their molecular and functional effects in this research.
Snippet: he desired mutations of hGAT‐1 were created using the QuikChange Lightning site‐directed mutagenesis kit (Agilent Technologies, Santa Clara, USA;cat. no. cat. no. 210518), using the WT hGAT‐1 (OriGene, cat. no. RC206290) inserted into the pEYFP‐C1 vector (Addgene;cat. no. 6006–1) (YFP‐hGAT‐1) as a template. The mutagenic primers (sequences shown are the sense strands in the 5′ to
FigureFigureFigure
Astrocytic γ-aminobutyric acid (GABA) transporters mediate guanidinoacetate transport in rat brain
Tachikawa, Yashiki, Akanuma et al
Neurochem. Int. (2017) 113, 1-7 DOI: 10.1016/j.neuint.2017.11.013
Cited by 14
AI Summary: OriGene’s human GAT1, GAT2, GAT3, and BGT1 cDNA constructs were used to express these GABA transporters in CHO cells, enabling the study to assess their roles in guanidinoacetate transport.
Snippet: Constructs of human GAT1-3 and BGT1 cDNAs (ORIGENE, Rockville, MD, USA) were introduced into the CHO cells with Effectene transfection reagent (QIAGEN Co., Hilden, Germany) according to the manufacturer's protocol
Showing 1-3 of 3 papers.
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