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KLHDC10 (NM_014997) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC206230
KLHDC10 (Myc-DDK-tagged)-Human kelch domain containing 10 (KLHDC10)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "KLHDC10 or NM_014997"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KLHDC10
Synonyms slim
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206230 representing NM_014997
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCCGCCCAGGGCTGGGACAGGAACCGCCGGAGGGGAGGAGGCGCCGCCGGCGCTGGTGGCGGAG
GTAGCGGGGCCGGCGGGGGCAGTGGGGGCAGCGGGGGTCGGGGGACTGGCCAGCTCAACCGCTTCGTGCA
ACTCTCCGGGCGGCCGCACCTGCCAGGTAAGAAGAAAATACGATGGGACCCAGTTAGGAGGCGCTTCATT
CAGTCCTGTCCCATCATAAGGATCCCTAACAGGTTTTTGAGAGGCCACAGACCTCCACCAGCACGAAGTG
GACATCGTTGTGTGGCAGATAATACCAACCTATATGTGTTTGGAGGTTATAACCCAGATTATGATGAATC
GGGAGGGCCTGATAATGAAGACTATCCTCTCTTCAGGGAACTCTGGAGGTATCATTTTGCTACAGGAGTA
TGGCACCAGATGGGCACAGATGGCTACATGCCCCGGGAATTGGCATCTATGTCACTTGTGCTGCATGGAA
ACAACCTGTTAGTATTTGGAGGTACGGGCATCCCATTTGGAGAGAGCAACGGCAATGACGTCCATGTGTG
TAATGTGAAGTATAAGAGATGGGCTTTGCTCAGCTGTCGGGGGAAGAAACCCAGTCGTATATATGGACAG
GCTATGGCCATCATCAATGGCTCCCTTTATGTCTTTGGAGGTACAACCGGCTATATTTACAGCACAGACC
TGCACAAGTTAGATCTCAATACCAGAGAGTGGACACAACTGAAACCAAACAACCTATCCTGTGATCTACC
AGAAGAGAGATACCGACATGAAATTGCACATGACGGGCAGAGGATTTACATCTTGGGAGGTGGTACTTCC
TGGACAGCATATTCCTTAAACAAGATCCATGCATACAACCTTGAAACGAATGCCTGGGAGGAAATTGCAA
CAAAACCCCATGAAAAAATAGGCTTTCCTGCAGCCCGAAGGTGTCACAGTTGTGTTCAAATAAAAAATGA
TGTATTTATTTGTGGGGGCTATAATGGAGAGGTGATCCTGGGAGATATCTGGAAGTTGAATCTGCAGACT
TTCCAATGGGTGAAGCTCCCAGCTACCATGCCAGAGCCAGTTTATTTTCACTGTGCAGCTGTTACACCAG
CTGGTTGCATGTACATTCATGGAGGAGTGGTGAACATCCATGAAAACAAACGGACTGGGTCATTGTTTAA
GATCTGGCTGGTGGTACCTAGCCTGCTGGAACTGGCATGGGAGAAGCTGCTTGCGGCCTTCCCTAACCTT
GCAAACCTCTCCCGAACACAACTTCTGCACCTTGGACTCACACAGGGACTCATCGAACGCTTGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206230 representing NM_014997
Red=Cloning site Green=Tags(s)

MSAAQGWDRNRRRGGGAAGAGGGGSGAGGGSGGSGGRGTGQLNRFVQLSGRPHLPGKKKIRWDPVRRRFI
QSCPIIRIPNRFLRGHRPPPARSGHRCVADNTNLYVFGGYNPDYDESGGPDNEDYPLFRELWRYHFATGV
WHQMGTDGYMPRELASMSLVLHGNNLLVFGGTGIPFGESNGNDVHVCNVKYKRWALLSCRGKKPSRIYGQ
AMAIINGSLYVFGGTTGYIYSTDLHKLDLNTREWTQLKPNNLSCDLPEERYRHEIAHDGQRIYILGGGTS
WTAYSLNKIHAYNLETNAWEEIATKPHEKIGFPAARRCHSCVQIKNDVFICGGYNGEVILGDIWKLNLQT
FQWVKLPATMPEPVYFHCAAVTPAGCMYIHGGVVNIHENKRTGSLFKIWLVVPSLLELAWEKLLAAFPNL
ANLSRTQLLHLGLTQGLIERLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014997
ORF Size 1326 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014997.4
RefSeq Size 6438 bp
RefSeq ORF 1329 bp
Locus ID 23008
UniProt ID Q6PID8
Cytogenetics 7q32.2
MW 49.1 kDa
Summary Participates in the oxidative stress-induced cell death through MAP3K5 activation. Inhibits PPP5C phosphatase activity on MAP3K5.UniProtKB/Swiss-Prot Function
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Documents
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"NM_014997" in other vectors (4)
SKU Description Size Price
RC206230L3 Lenti-ORF clone of KLHDC10 (Myc-DDK-tagged)-Human kelch domain containing 10 (KLHDC10) 10 ug
$757.00
RC206230L4 Lenti-ORF clone of KLHDC10 (mGFP-tagged)-Human kelch domain containing 10 (KLHDC10) 10 ug
$757.00
RG206230 KLHDC10 (tGFP-tagged) - Human kelch domain containing 10 (KLHDC10) 10 ug
$657.00
SC304201 KLHDC10 (untagged)-Human kelch domain containing 10 (KLHDC10) 10 ug
$503.00

Plasmid Map for RC206230

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206230
Circular map for RC206230

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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