OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

BBS4 (NM_033028) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC206210
BBS4 (Myc-DDK-tagged)-Human Bardet-Biedl syndrome 4 (BBS4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $483.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA804560 BBS4 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5) 100 ul
$447.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "BBS4 or NM_033028"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BBS4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206210 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGGAGAGAGTCGCGACGAGAACTCAATTTCCTGTATCTACTGAGTCTCAAAAACCCCGGCAGA
AAAAAGCTCCAGAGTTTCCTATTTTGGAGAAGCAGAACTGGTTGATTCATCTTCATTATATCCGGAAAGA
TTATGAAGCCTGCAAGGCTGTTATCAAAGAACAGCTTCAAGAGACTCAGGGATTGTGTGAATATGCTATC
TATGTCCAAGCATTGATATTTCGCCTAGAAGGAAATATCCAAGAATCCCTAGAACTCTTCCAGACATGTG
CAGTTCTTAGTCCTCAGAGTGCTGATAACCTCAAGCAGGTGGCCAGATCTTTATTTCTTTTGGGAAAACA
TAAAGCTGCCATTGAAGTATATAATGAAGCAGCTAAACTCAACCAGAAAGATTGGGAGATCAGCCATAAC
CTAGGAGTTTGCTACATATACCTGAAGCAGTTCAACAAGGCACAAGACCAGTTGCACAATGCCCTGAATC
TTAATAGGCACGATCTGACTTATATAATGCTGGGGAAGATCCACTTGCTGGAGGGAGACTTGGACAAGGC
CATTGAAGTCTACAAGAAAGCAGTGGAGTTCTCACCAGAAAATACAGAGCTTCTTACAACTTTAGGATTA
CTCTACTTACAGCTCGGCATTTACCAGAAGGCATTTGAACATCTTGGCAATGCACTGACTTATGACCCTA
CCAACTACAAGGCCATCTTGGCAGCAGGCAGCATGATGCAGACCCACGGGGACTTTGATGTTGCCCTCAC
CAAATACAGAGTTGTGGCTTGTGCTGTTCCAGAAAGTCCTCCACTCTGGAATAACATTGGAATGTGTTTC
TTTGGCAAGAAGAAATATGTGGCGGCCATCAGCTGCCTGAAACGAGCCAACTACTTGGCACCCTTCGATT
GGAAGATTCTGTATAATTTGGGCCTTGTCCATTTGACCATGCAGCAGTATGCATCAGCTTTTCATTTTCT
CAGTGCGGCCATCAACTTCCAGCCAAAGATGGGGGAGCTCTACATGCTCTTGGCAGTGGCTCTGACCAAT
CTGGAAGATACAGAAAATGCCAAGAGAGCCTACGCAGAAGCAGTCCACCTGGATAAGTGTAACCCTTTAG
TAAACCTGAACTATGCTGTGCTGCTGTACAACCAGGGCGAGAAGAAGAACGCCCTGGTCCAATATCAGGA
GATGGAGAAGAAAGTCAGCCTACTCAAGGACAATAGCTCTCTGGAATTTGACTCTGAGATGGTGGAGATG
GCTCAGAAGTTGGGAGCTGCTCTCCAGGTTGGGGAGGCACTGGTCTGGACCAAACCAGTTAAAGATCCCA
AATCAAAGCACCAGACCACTTCAACCAGCAAACCTGCCAGTTTCCAGCAGCCTCTGGGCTCTAATCAAGC
TCTAGGACAGGCAATGTCTTCAGCAGCTGCATACAGGACGCTCCCCTCAGGTGCTGGAGGAACATCCCAG
TTCACAAAGCCCCCATCTCTTCCTCTGGAGCCAGAGCCTGCGGTGGAATCAAGTCCAACTGAAACATCAG
AACAAATAAGAGAGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206210 protein sequence
Red=Cloning site Green=Tags(s)

MAEERVATRTQFPVSTESQKPRQKKAPEFPILEKQNWLIHLHYIRKDYEACKAVIKEQLQETQGLCEYAI
YVQALIFRLEGNIQESLELFQTCAVLSPQSADNLKQVARSLFLLGKHKAAIEVYNEAAKLNQKDWEISHN
LGVCYIYLKQFNKAQDQLHNALNLNRHDLTYIMLGKIHLLEGDLDKAIEVYKKAVEFSPENTELLTTLGL
LYLQLGIYQKAFEHLGNALTYDPTNYKAILAAGSMMQTHGDFDVALTKYRVVACAVPESPPLWNNIGMCF
FGKKKYVAAISCLKRANYLAPFDWKILYNLGLVHLTMQQYASAFHFLSAAINFQPKMGELYMLLAVALTN
LEDTENAKRAYAEAVHLDKCNPLVNLNYAVLLYNQGEKKNALVQYQEMEKKVSLLKDNSSLEFDSEMVEM
AQKLGAALQVGEALVWTKPVKDPKSKHQTTSTSKPASFQQPLGSNQALGQAMSSAAAYRTLPSGAGGTSQ
FTKPPSLPLEPEPAVESSPTETSEQIREK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033028
ORF Size 1557 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033028.5
RefSeq Size 2515 bp
RefSeq ORF 1560 bp
Locus ID 585
UniProt ID Q96RK4, A0A0S2Z3A9
Cytogenetics 15q24.1
Domains TPR
MW 58.3 kDa
Summary This gene is a member of the Bardet-Biedl syndrome (BBS) gene family. Bardet-Biedl syndrome is an autosomal recessive disorder characterized by severe pigmentary retinopathy, obesity, polydactyly, renal malformation and cognitive disability. The proteins encoded by BBS gene family members are structurally diverse. The similar phenotypes exhibited by mutations in BBS gene family members are likely due to the protein's shared roles in cilia formation and function. Many BBS proteins localize to the basal bodies, ciliary axonemes, and pericentriolar regions of cells. BBS proteins may also be involved in intracellular trafficking via microtubule-related transport. The protein encoded by this gene has sequence similarity to O-linked N-acetylglucosamine (O-GlcNAc) transferases in plants and archaebacteria and in human forms a multi-protein "BBSome" complex with seven other BBS proteins. Alternate splicing results in multiple transcript variants. provided by RefSeq, Mar 2016
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_033028" in other vectors (4)
SKU Description Size Price
RC206210L3 Lenti ORF clone of Human Bardet-Biedl syndrome 4 (BBS4), Myc-DDK-tagged 10 ug
$783.00
RC206210L4 Lenti ORF clone of Human Bardet-Biedl syndrome 4 (BBS4), mGFP tagged 10 ug
$783.00
RG206210 BBS4 (tGFP-tagged) - Human Bardet-Biedl syndrome 4 (BBS4) 10 ug
$489.00 $683.00
SC127001 BBS4 (untagged)-Human Bardet-Biedl syndrome 4 (BBS4) 10 ug
$484.00

Plasmid Map for RC206210

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206210
Circular map for RC206210

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products