RFXDC1 (RFX6) (NM_173560) Human Tagged ORF Clone
$850.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RFXDC1 |
| Synonyms | dJ955L16.1; MTCHRS; MTFS; RFXDC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_173560 |
| ORF Size | 2784 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_173560.1, NP_775831.1 |
| RefSeq Size | 3517 bp |
| RefSeq ORF | 2787 bp |
| Locus ID | 222546 |
| UniProt ID | Q8HWS3 |
| Cytogenetics | 6q22.1 |
| Protein Families | Transcription Factors |
| MW | 102.4 kDa |
| Summary | The nuclear protein encoded by this gene is a member of the regulatory factor X (RFX) family of transcription factors. Studies in mice suggest that this gene is specifically required for the differentiation of islet cells for the production of insulin, but not for the differentiation of pancreatic polypeptide-producing cells. It regulates the transcription factors involved in beta-cell maturation and function, thus, restricting the expression of the beta-cell differentiation and specification genes. Mutations in this gene are associated with Mitchell-Riley syndrome, which is characterized by neonatal diabetes with pancreatic hypoplasia, duodenal and jejunal atresia, and gall bladder agenesis.provided by RefSeq, Sep 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_173560" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206174L3 | Lenti ORF clone of Human regulatory factor X, 6 (RFX6), Myc-DDK-tagged | 10 ug |
$1,150.00
|
|
| RC206174L4 | Lenti ORF clone of Human regulatory factor X, 6 (RFX6), mGFP tagged | 10 ug |
$1,150.00
|
|
| RG206174 | RFX6 (tGFP-tagged) - Human regulatory factor X, 6 (RFX6) | 10 ug |
$1,050.00
|
|
| SC126045 | RFX6 (untagged)-Human regulatory factor X, 6 (RFX6) | 10 ug |
$851.00
|
|
Citations
Deletion of RFX6, a Diabetes-Associated Gene, Impairs iPSC-Derived Islet Organoid Development and Survival, With No Impact on the Generation of PDX1+/NKX6.1+ Progenitors
Aldous, Elsayed, Memon et al
bioRxiv (2024)
Aldous, Elsayed, Memon et al
bioRxiv (2024)
Snippet: Cells were trypsinized and resuspended in differentiation media with 10 µM Y-27632. Resuspended cells were transfected with either RFX6 plasmid (RFX6 (Myc-DDK-tagged), RC206174, OriGene, USA) or empty vector. …
Identification of novel, clinically correlated autoantigens in the monogenic autoimmune syndrome APS1 by proteome-wide PhIP-Seq
Vazquez, Ferré, Scheel et al
Elife (2020) 9 DOI: 10.7554/eLife.55053
Vazquez, Ferré, Scheel et al
Elife (2020) 9 DOI: 10.7554/eLife.55053
AI Summary: OriGene products, specifically the 293T mock transfection construct (PS100001) and the 293T RFX6 transfection construct (RC206174), were used in this study to facilitate the identification and validation of novel autoantigens in APS1.
Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Transfected construct (human) 293T mock transfection construct Origene PS100001 Transfected construct (human) 293T RFX6 transfection construct Origene RC206174 Antibody Goat Anti-NLRP5, polyclonal Santa Cruz sc-50630 RLBA 1:50 Antibody Mouse Anti-SOX10, monoclonal Abcam ab181466 RLBA 1:25
Identification of novel, clinically correlated autoantigens in the monogenic autoimmune syndrome APS1 by PhIP-Seq
Vazquez, Ferre, Scheel et al
bioRxiv (2020)
Vazquez, Ferre, Scheel et al
bioRxiv (2020)
Snippet: …For transfections, 0.1ug of sequence-verified pCMV-insert-MYC-FLAG overexpression vectors containing either no insert (Origene #PS100001;‘mock’ transfection) or RFX6 insert (Origene #RC206174) were transfected into each well. 24 hours post-transfection, cells were washed in 1X PBS and fixed in 4% PFA for 10 minutes at room temperature
Showing 1-3 of 3 papers.
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