RFXDC1 (RFX6) (NM_173560) Human Tagged ORF Clone

SKU RC206174

RFX6 (Myc-DDK-tagged)-Human regulatory factor X, 6 (RFX6)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: RFXDC1
• Synonyms: dJ955L16.1; MTCHRS; MTFS; RFXDC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206174 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCAAGGTCCCGAAGCTGGAAGACACCTTCCTGCAGGCGCAGCCTGCGCCCCAACTGTCCCCGGGGA; TCCAGGAAGACTGCTGTGTGCAGCTCCTGGGCAAGGGCTTGCTAGTCTATCCGGAAGAAACAGTGTACCT; GGCGGCCGAAGGGCAGCCCGGGGGCGAGCAGGGCGGCGGGGAGAAAGGCGAAGACCCGGAGCTGCCGGGG; GCAGTGAAATCAGAAATGCACTTAAACAATGGTAACTTTTCCTCTGAAGAAGAGGACGCCGACAACCACG; ACAGCAAAACCAAAGCAGCGGATCAATACCTGTCTCAGAAGAAAACCATCACGCAGATTGTGAAGGATAA; AAAGAAGCAGACACAGCTCACGCTGCAGTGGCTTGAAGAGAATTACATTGTATGTGAAGGAGTTTGCTTA; CCACGGTGCATTCTTTATGCACACTACTTAGATTTCTGTAGGAAAGAGAAATTAGAGCCAGCCTGTGCGG; CCACCTTTGGAAAGACAATTCGCCAGAAGTTTCCCCTCCTAACAACAAGGCGGCTTGGAACAAGAGGCCA; TTCAAAGTATCATTACTATGGGATTGGCATCAAAGAGAGCAGTGCATATTACCACTCCGTTTATTCTGGA; AAGGGCTTGACAAGGTTTTCTGGAAGCAAGCTAAAGAATGAGGGTGGCTTCACTCGTAAATATTCGCTTA; GCTCAAAAACTGGAACACTTCTTCCAGAATTCCCCAGCGCTCAACACCTTGTATACCAAGGATGCATTTC; TAAGGACAAGGTTGATACGCTCATAATGATGTACAAAACTCACTGCCAGTGTATCCTGGACAATGCAATT; AATGGAAACTTTGAAGAGATCCAGCATTTTTTATTACACTTTTGGCAAGGAATGCCTGACCATCTCCTTC; CCCTGCTCGAAAATCCTGTTATCATTGATATTTTCTGTGTTTGTGACTCAATTCTTTATAAGGTTCTTAC; AGATGTACTCATTCCTGCAACAATGCAAGAAATGCCTGAAAGCTTATTAGCAGACATAAGAAATTTTGCT; AAAAATTGGGAACAGTGGGTTGTTTCATCCTTGGAAAACTTGCCAGAAGCTCTAACTGACAAGAAAATAC; CTATTGTGCGAAGATTTGTATCTTCTCTGAAACGACAAACATCTTTCTTACATCTTGCCCAGATTGCCAG; ACCAGCTCTCTTTGACCAGCATGTCGTTAATTCTATGGTGTCTGATATTGAAAGGGTTGATTTGAACAGC; ATTGGCTCTCAAGCCCTTCTTACCATTTCAGGCAGCACAGACACTGAATCTGGTATCTACACTGAACATG; ACTCTATCACTGTGTTCCAAGAACTGAAGGATCTCCTTAAGAAGAATGCCACTGTGGAGGCTTTTATTGA; ATGGTTGGATACTGTGGTAGAACAGAGAGTTATTAAGACCAGCAAACAAAATGGAAGGTCATTAAAGAAG; AGAGCTCAAGACTTTCTGTTAAAGTGGAGTTTTTTTGGTGCTCGAGTAATGCATAATCTCACCTTGAACA; ATGCATCCAGTTTTGGTTCTTTTCATTTGATTCGAATGCTTCTCGATGAATACATTCTCCTGGCCATGGA; GACCCAGTTTAATAATGACAAAGAGCAGGAGTTACAGAATTTATTGGACAAGTATATGAAGAATTCAGAT; GCGAGTAAAGCTGCTTTCACTGCTTCTCCGAGTTCATGCTTTCTGGCCAACCGTAATAAAGGGAGCATGG; TTTCCAGCGACGCTGTGAAGAATGAAAGCCACGTGGAGACAACCTATCTCCCTCTGCCATCCAGTCAACC; TGGAGGCCTAGGCCCTGCTCTGCACCAGTTCCCTGCTGGGAACACAGACAACATGCCGCTCACAGGTCAA; ATGGAGCTTTCACAGATTGCTGGTCATCTGATGACACCACCCATTTCTCCAGCCATGGCAAGCCGAGGAA; GTGTCATTAACCAAGGACCAATGGCAGGGAGGCCCCCAAGTGTGGGCCCAGTACTGTCAGCTCCATCACA; CTGCTCCACATACCCAGAGCCCATTTATCCCGCTCTCCCTCAAGCCAATCATGACTTTTATAGCACCAGC; TCTAACTACCAGACTGTGTTTAGGGCACAGCCCCACTCCACATCAGGACTCTATCCTCATCACACCGAGC; ATGGTCGATGCATGGCTTGGACTGAACAGCAGCTTTCAAGAGACTTCTTCAGTGGCAGCTGTGCGGGGTC; TCCATATAACTCCCGGCCACCGTCTAGCTATGGCCCATCCCTGCAAGCCCAGGATTCACACAATATGCAG; TTTTTAAATACAGGAAGCTTCAATTTCTTGAGCAACACAGGAGCTGCCAGCTGCCAAGGAGCAACACTGC; CTCCTAATTCACCAAATGGATACTATGGAAGCAACATAAACTACCCAGAGTCTCACAGGCTCGGATCAAT; GGTGAATCAGCACGTTTCTGTCATCAGCAGCATTCGTTCACTGCCCCCCTACAGTGACATCCACGATCCA; CTTAACATTTTAGATGACAGTGGTAGAAAACAGACCAGCTCGTTTTACACAGACACATCATCTCCAGTTG; CATGTCGAACTCCAGTCCTAGCTTCCAGTTTGCAAACCCCAATTCCTTCTTCCTCATCCCAATGTATGTA; TGGAACTTCCAACCAGTATCCAGCTCAAGAAACCCTGGACTCCCATGGAACAAGCAGTAGAGAAATGGTG; TCCTCTTTACCACCTATCAACACTGTGTTCATGGGAACAGCAGCTGGAGGCACT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206174 protein sequence; Red=Cloning site Green=Tags(s); ; MAKVPKLEDTFLQAQPAPQLSPGIQEDCCVQLLGKGLLVYPEETVYLAAEGQPGGEQGGGEKGEDPELPG; AVKSEMHLNNGNFSSEEEDADNHDSKTKAADQYLSQKKTITQIVKDKKKQTQLTLQWLEENYIVCEGVCL; PRCILYAHYLDFCRKEKLEPACAATFGKTIRQKFPLLTTRRLGTRGHSKYHYYGIGIKESSAYYHSVYSG; KGLTRFSGSKLKNEGGFTRKYSLSSKTGTLLPEFPSAQHLVYQGCISKDKVDTLIMMYKTHCQCILDNAI; NGNFEEIQHFLLHFWQGMPDHLLPLLENPVIIDIFCVCDSILYKVLTDVLIPATMQEMPESLLADIRNFA; KNWEQWVVSSLENLPEALTDKKIPIVRRFVSSLKRQTSFLHLAQIARPALFDQHVVNSMVSDIERVDLNS; IGSQALLTISGSTDTESGIYTEHDSITVFQELKDLLKKNATVEAFIEWLDTVVEQRVIKTSKQNGRSLKK; RAQDFLLKWSFFGARVMHNLTLNNASSFGSFHLIRMLLDEYILLAMETQFNNDKEQELQNLLDKYMKNSD; ASKAAFTASPSSCFLANRNKGSMVSSDAVKNESHVETTYLPLPSSQPGGLGPALHQFPAGNTDNMPLTGQ; MELSQIAGHLMTPPISPAMASRGSVINQGPMAGRPPSVGPVLSAPSHCSTYPEPIYPALPQANHDFYSTS; SNYQTVFRAQPHSTSGLYPHHTEHGRCMAWTEQQLSRDFFSGSCAGSPYNSRPPSSYGPSLQAQDSHNMQ; FLNTGSFNFLSNTGAASCQGATLPPNSPNGYYGSNINYPESHRLGSMVNQHVSVISSIRSLPPYSDIHDP; LNILDDSGRKQTSSFYTDTSSPVACRTPVLASSLQTPIPSSSSQCMYGTSNQYPAQETLDSHGTSSREMV; SSLPPINTVFMGTAAGGT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173560
• ORF Size: 2784 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_173560.1, NP_775831.1
• RefSeq Size: 3517 bp
• RefSeq ORF: 2787 bp
• Locus ID: 222546
• UniProt ID: Q8HWS3
• Cytogenetics: 6q22.1
• Protein Families: Transcription Factors
• MW: 102.4 kDa
• Summary: The nuclear protein encoded by this gene is a member of the regulatory factor X (RFX) family of transcription factors. Studies in mice suggest that this gene is specifically required for the differentiation of islet cells for the production of insulin, but not for the differentiation of pancreatic polypeptide-producing cells. It regulates the transcription factors involved in beta-cell maturation and function, thus, restricting the expression of the beta-cell differentiation and specification genes. Mutations in this gene are associated with Mitchell-Riley syndrome, which is characterized by neonatal diabetes with pancreatic hypoplasia, duodenal and jejunal atresia, and gall bladder agenesis.[provided by RefSeq, Sep 2010]