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MFSD8 (NM_152778) Human Tagged ORF Clone

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SKU
RC206118
MFSD8 (Myc-DDK-tagged)-Human major facilitator superfamily domain containing 8 (MFSD8)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $482.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "MFSD8 or NM_152778"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MFSD8
Synonyms CCMD; CLN7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206118 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCCTGCGGAACGAAAGTGAACAGGAGCCGCTCTTAGGCGACACACCTGGAAGCAGAGAATGGG
ACATTTTAGAGACTGAAGAGCATTATAAGAGCCGATGGAGATCTATTAGGATTTTATATCTTACTATGTT
TCTCAGCAGTGTAGGGTTTTCTGTAGTGATGATGTCCATATGGCCATATCTCCAAAAGATTGATCCGACA
GCTGATACAAGTTTTTTGGGCTGGGTTATTGCTTCATATAGTCTTGGCCAAATGGTAGCTTCACCTATAT
TTGGTTTATGGTCTAATTATAGACCAAGAAAAGAGCCTCTTATTGTCTCCATCTTGATTTCCGTGGCAGC
CAACTGCCTCTATGCATATCTCCACATCCCAGCTTCTCATAATAAATACTACATGCTGGTTGCTCGTGGA
TTGTTGGGAATTGGAGCAGGAAATGTAGCAGTTGTTAGATCATATACTGCTGGTGCTACTTCCCTTCAGG
AAAGAACAAGTTCCATGGCAAACATAAGCATGTGTCAAGCATTAGGTTTTATTCTAGGTCCAGTTTTTCA
GACTTGTTTTACATTCCTTGGAGAAAAAGGTGTGACATGGGATGTGATTAAACTGCAGATAAACATGTAT
ACAACACCAGTTTTACTTAGCGCCTTCCTGGGAATTTTAAATATTATTCTGATCCTTGCCATACTAAGAG
AACATCGTGTGGATGACTCAGGAAGACAGTGTAAAAGTATTAATTTTGAAGAAGCAAGTACAGATGAAGC
TCAGGTTCCCCAAGGAAATATTGACCAGGTTGCTGTTGTGGCCATCAATGTTCTGTTTTTTGTGACTCTA
TTTATCTTTGCCCTTTTTGAAACCATCATTACTCCATTAACAATGGATATGTATGCCTGGACTCAAGAAC
AAGCTGTGTTATATAATGGCATAATACTTGCTGCTCTTGGGGTTGAAGCCGTTGTTATTTTCTTAGGAGT
TAAGTTGCTTTCCAAAAAGATTGGCGAGCGTGCTATTCTACTGGGAGGACTCATCGTTGTATGGGTTGGC
TTCTTTATCTTGTTACCTTGGGGAAATCAATTTCCCAAAATACAGTGGGAAGATTTGCACAATAATTCAA
TCCCTAATACCACATTTGGGGAAATTATTATTGGTCTTTGGAAGTCTCCAATGGAAGATGACAATGAAAG
ACCAACTGGTTGCTCGATTGAACAAGCCTGGTGCCTCTACACCCCGGTGATTCATCTGGCCCAGTTCCTT
ACATCAGCTGTGCTAATAGGATTAGGCTATCCAGTCTGCAATCTTATGTCCTATACTCTATATTCAAAAA
TTCTAGGACCAAAACCTCAGGGTGTATACATGGGCTGGTTAACAGCATCTGGAAGTGGAGCCCGGATTCT
TGGGCCTATGTTCATCAGCCAAGTGTATGCTCACTGGGGACCACGATGGGCATTCAGCCTGGTGTGTGGA
ATAATAGTGCTCACCATCACCCTCCTGGGAGTGGTTTACAAAAGACTCATTGCTCTTTCTGTAAGATATG
GGAGGATTCAGGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206118 protein sequence
Red=Cloning site Green=Tags(s)

MAGLRNESEQEPLLGDTPGSREWDILETEEHYKSRWRSIRILYLTMFLSSVGFSVVMMSIWPYLQKIDPT
ADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYLHIPASHNKYYMLVARG
LLGIGAGNVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPVFQTCFTFLGEKGVTWDVIKLQINMY
TTPVLLSAFLGILNIILILAILREHRVDDSGRQCKSINFEEASTDEAQVPQGNIDQVAVVAINVLFFVTL
FIFALFETIITPLTMDMYAWTQEQAVLYNGIILAALGVEAVVIFLGVKLLSKKIGERAILLGGLIVVWVG
FFILLPWGNQFPKIQWEDLHNNSIPNTTFGEIIIGLWKSPMEDDNERPTGCSIEQAWCLYTPVIHLAQFL
TSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGPMFISQVYAHWGPRWAFSLVCG
IIVLTITLLGVVYKRLIALSVRYGRIQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152778
ORF Size 1554 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152778.3
RefSeq Size 4562 bp
RefSeq ORF 1557 bp
Locus ID 256471
UniProt ID Q8NHS3
Cytogenetics 4q28.2
Domains sugar_tr
Protein Families Transmembrane
Protein Pathways Lysosome
MW 57.6 kDa
Summary This gene encodes a ubiquitous integral membrane protein that contains a transporter domain and a major facilitator superfamily (MFS) domain. Other members of the major facilitator superfamily transport small solutes through chemiosmotic ion gradients. The substrate transported by this protein is unknown. The protein likely localizes to lysosomal membranes. Mutations in this gene are correlated with a variant form of late infantile-onset neuronal ceroid lipofuscinoses (vLINCL). provided by RefSeq, Oct 2008
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Documents
Resources
"NM_152778" in other vectors (4)
SKU Description Size Price
RC206118L3 Lenti ORF clone of Human major facilitator superfamily domain containing 8 (MFSD8), Myc-DDK-tagged 10 ug
$782.00
RC206118L4 Lenti ORF clone of Human major facilitator superfamily domain containing 8 (MFSD8), mGFP tagged 10 ug
$782.00
RG206118 MFSD8 (tGFP-tagged) - Human major facilitator superfamily domain containing 8 (MFSD8) 10 ug
$489.00 $682.00
SC100793 MFSD8 (untagged)-Human major facilitator superfamily domain containing 8 (MFSD8) 10 ug
$483.00

Plasmid Map for RC206118

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206118
Circular map for RC206118

file-alt Citations

MFSD6 is an entry receptor for enterovirus D68
Varanese, Xu, Peters et al
Nature (2025)
Cited by 34
AI Summary: OriGene contributed to this study by providing Myc- and Flag-tagged MFSD6 (RC219252) and MFSD8 (RC206118) overexpression constructs, which were used to investigate the role of MFSD6 as an entry receptor for enterovirus D68.
Snippet: For cloning overexpression constructs, MFSD6 (RC219252) and MFSD8 (RC206118) tagged 777 with Myc and Flag on the C terminus were ordered through Origene. T
Showing 1-1 of 1 papers.
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