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RRP1B (NM_015056) Human Tagged ORF Clone

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SKU
RC206073
RRP1B (Myc-DDK-tagged)-Human ribosomal RNA processing 1 homolog B (S. cerevisiae) (RRP1B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $694.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RRP1B
Synonyms KIAA0179; Nnp1; NNP1L; PPP1R136; RRP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206073 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCCGCCATGCAGCCGGCCGAGATCCAATTTGCCCAGCGGCTGGCGTCCAGCGAGAAGGGCATCC
GGGACCGAGCGGTGAAGAAGCTGCGCCAGTACATCAGCGTGAAGACGCAGAGGGAGACAGGAGGTTTCAG
TCAGGAAGAACTTCTGAAAATCTGGAAGGGGCTCTTCTACTGCATGTGGGTGCAGGATGAACCCCTTCTA
CAGGAAGAGCTCGCCAACACCATTGCACAGCTAGTCCATGCTGTTAACAACTCAGCGGCTCAACACCTGT
TCATTCAGACCTTTTGGCAAACCATGAATCGAGAATGGAAAGGAATAGACAGGCTACGCCTGGACAAATA
CTATATGCTGATTCGTCTGGTCCTGAGGCAGTCCTTTGAAGTCTTGAAGCGAAATGGCTGGGAAGAAAGC
CGAATCAAGGTTTTCTTGGATGTCCTGATGAAGGAGGTCCTGTGTCCTGAGAGTCAGTCTCCTAATGGAG
TGAGATTCCACTTCATTGATATTTACCTGGATGAACTCTCCAAAGTCGGGGGGAAGGAGCTTTTAGCAGA
TCAGAATCTCAAGTTTATCGATCCATTCTGCAAAATTGCTGCAAAGACGAAGGACCACACCCTGGTACAG
ACCATAGCTCGGGGTGTCTTCGAAGCTATCGTAGATCAGTCTCCTTTTGTGCCTGAAGAGACGATGGAGG
AACAGAAGACAAAAGTGGGTGATGGTGACCTCTCTGCTGAGGAGATACCTGAAAATGAGGTATCCTTGAG
AAGAGCTGTCAGTAAAAAGAAGACAGCACTGGGCAAAAACCATTCCAGAAAAGATGGACTCAGTGATGAA
AGAGGAAGAGATGACTGTGGAACCTTTGAGGACACAGGGCCCCTTCTCCAGTTTGACTATAAGGCTGTTG
CTGATCGACTCCTGGAAATGACCAGCAGGAAGAACACGCCCCACTTCAACAGGAAGCGCCTCTCCAAACT
CATCAAGAAATTCCAAGACCTTTCTGAAGGAAGCAGTATATCTCAACTCAGTTTTGCGGAGGACATTTCT
GCTGATGAAGATGACCAAATCCTCAGTCAAGGAAAGCATAAGAAGAAAGGAAATAAACTTTTAGAGAAAA
CTAACTTGGAAAAGGAGAAAGGAAGCAGAGTCTTTTGTGTAGAGGAAGAGGACAGTGAAAGCAGTCTTCA
AAAGAGAAGAAGGAAGAAGAAGAAGAAGCACCACCTGCAGCCTGAAAATCCAGGCCCAGGGGGTGCAGCC
CCGTCCCTGGAACAGAACCGGGGCAGGGAGCCCGAGGCCTCTGGGCCGAAAGCCCTGAAGGCACGTGTGG
CCGAGCCAGGTGCAGAGGCCACGTCCAGCACTGGGGAGGAGAGTGGCTCCGAGCATCCTCCAGCCGTCCC
CATGCACAATAAAAGGAAACGGCCACGGAAGAAGAGCCCGAGGGCCCACAGGGAAATGTTGGAATCAGCA
GTGTTGCCCCCAGAGGACATGTCTCAGAGTGGCCCGAGTGGCAGTCATCCTCAGGGACCTAGAGGGTCCC
CGACAGGTGGAGCCCAACTCCTAAAAAGGAAGCGGAAACTTGGAGTTGTGCCCGTCAATGGCAGTGGCCT
GTCCACGCCGGCCTGGCCTCCATTGCAGCAGGAAGGCCCTCCCACAGGCCCCGCAGAGGGGGCGAACAGC
CACACCACGCTGCCCCAGCGCAGGAGGCTGCAGAAAAAGAAGGCAGGGCCCGGCAGCCTGGAGCTCTGTG
GCCTGCCCAGCCAGAAAACAGCAAGTTTGAAAAAGAGGAAGAAAATGAGAGTGATGTCAAACTTGGTGGA
GCACAACGGGGTGCTGGAGTCCGAAGCTGGGCAACCCCAGGCTCTGGGAAGCAGTGGGACTTGCAGTTCC
CTGAAGAAGCAGAAGCTGAGGGCAGAGAGCGACTTTGTGAAGTTTGACACCCCCTTCTTACCAAAGCCCC
TGTTCTTCAGAAGAGCCAAGAGCAGCACTGCCACCCACCCTCCAGGCCCTGCCGTCCAGCTAAACAAGAC
ACCATCCAGCTCCAAGAAAGTCACCTTTGGGCTGAACAGAAACATGACTGCCGAATTCAAGAAGACAGAC
AAGAGTATCTTGGTCAGTCCCACGGGCCCTTCTCGAGTGGCCTTCGACCCTGAACAGAAGCCCCTCCACG
GGGTGCTGAAGACCCCCACCAGCTCACCTGCCAGCTCACCCCTGGTGGCCAAGAAGCCCCTGACCACCAC
ACCAAGGAGAAGGCCCAGGGCTATGGATTTCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206073 protein sequence
Red=Cloning site Green=Tags(s)

MAPAMQPAEIQFAQRLASSEKGIRDRAVKKLRQYISVKTQRETGGFSQEELLKIWKGLFYCMWVQDEPLL
QEELANTIAQLVHAVNNSAAQHLFIQTFWQTMNREWKGIDRLRLDKYYMLIRLVLRQSFEVLKRNGWEES
RIKVFLDVLMKEVLCPESQSPNGVRFHFIDIYLDELSKVGGKELLADQNLKFIDPFCKIAAKTKDHTLVQ
TIARGVFEAIVDQSPFVPEETMEEQKTKVGDGDLSAEEIPENEVSLRRAVSKKKTALGKNHSRKDGLSDE
RGRDDCGTFEDTGPLLQFDYKAVADRLLEMTSRKNTPHFNRKRLSKLIKKFQDLSEGSSISQLSFAEDIS
ADEDDQILSQGKHKKKGNKLLEKTNLEKEKGSRVFCVEEEDSESSLQKRRRKKKKKHHLQPENPGPGGAA
PSLEQNRGREPEASGPKALKARVAEPGAEATSSTGEESGSEHPPAVPMHNKRKRPRKKSPRAHREMLESA
VLPPEDMSQSGPSGSHPQGPRGSPTGGAQLLKRKRKLGVVPVNGSGLSTPAWPPLQQEGPPTGPAEGANS
HTTLPQRRRLQKKKAGPGSLELCGLPSQKTASLKKRKKMRVMSNLVEHNGVLESEAGQPQALGSSGTCSS
LKKQKLRAESDFVKFDTPFLPKPLFFRRAKSSTATHPPGPAVQLNKTPSSSKKVTFGLNRNMTAEFKKTD
KSILVSPTGPSRVAFDPEQKPLHGVLKTPTSSPASSPLVAKKPLTTTPRRRPRAMDFF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015056
ORF Size 2274 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015056.3
RefSeq Size 5087 bp
RefSeq ORF 2277 bp
Locus ID 23076
UniProt ID Q14684
Cytogenetics 21q22.3
MW 84.4 kDa
Summary Positively regulates DNA damage-induced apoptosis by acting as a transcriptional coactivator of proapoptotic target genes of the transcriptional activator E2F1 (PubMed:20040599). Likely to play a role in ribosome biogenesis by targeting serine/threonine protein phosphatase PP1 to the nucleolus (PubMed:20926688). Involved in regulation of mRNA splicing (By similarity). Inhibits SIPA1 GTPase activity (By similarity). Involved in regulating expression of extracellular matrix genes (By similarity). Associates with chromatin and may play a role in modulating chromatin structure (PubMed:19710015).UniProtKB/Swiss-Prot Function
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"NM_015056" in other vectors (4)
SKU Description Size Price
RC206073L3 Lenti ORF clone of Human ribosomal RNA processing 1 homolog B (S. cerevisiae) (RRP1B), Myc-DDK-tagged 10 ug
$994.00
RC206073L4 Lenti ORF clone of Human ribosomal RNA processing 1 homolog B (S. cerevisiae) (RRP1B), mGFP tagged 10 ug
$994.00
RG206073 RRP1B (tGFP-tagged) - Human ribosomal RNA processing 1 homolog B (S. cerevisiae) (RRP1B) 10 ug
$894.00
SC304213 RRP1B (untagged)-Human ribosomal RNA processing 1 homolog B (S. cerevisiae) (RRP1B) 10 ug
$764.00

Plasmid Map for RC206073

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206073
Circular map for RC206073

file-alt Citations

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