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ZHX1 (NM_007222) Human Tagged ORF Clone

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SKU
RC206025
ZHX1 (Myc-DDK-tagged)-Human zinc fingers and homeoboxes 1 (ZHX1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $799.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZHX1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206025 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAGCAGGCGAAAATCAACAACACCTTGCATGGTCCTTGCCAGTGAACAAGATCCAGACCTTGAGT
TGATATCAGATTTGGATGAAGGTCCTCCTGTGCTTACACCTGTAGAAAACACCAGAGCAGAGAGTATCTC
AAGTGATGAAGAGGTTCATGAATCTGTGGATTCAGACAATCAGCAAAATAAAAAAGTTGAAGGTGGATAT
GAATGTAAATATTGTACTTTTCAAACTCCAGATCTAAATATGTTTACTTTTCATGTGGATTCGGAACATC
CCAATGTAGTGCTAAATTCATCCTATGTTTGTGTCGAATGCAATTTTCTTACCAAAAGGTATGATGCACT
TTCTGAGCATAATCTGAAATATCACCCAGGAGAAGAGAATTTTAAGTTGACTATGGTGAAACGTAATAAC
CAGACAATCTTTGAACAAACAATAAATGATCTGACTTTTGATGGTAGTTTTGTTAAAGAGGAGAATGCAG
AGCAAGCAGAATCTACAGAAGTTTCTTCTTCGGGAATATCTATCAGTAAAACTCCTATCATGAAAATGAT
GAAAAATAAAGTGGAAAATAAACGGATTGCAGTTCATCATAACTCAGTTGAGGACGTTCCTGAAGAGAAA
GAGAATGAAATCAAACCAGACCGTGAAGAAATTGTAGAAAATCCAAGTTCTTCAGCTTCTGAATCTAATA
CAAGTACTTCCATTGTAAACAGAATACATCCAAGTACTGCCAGCACGGTAGTGACACCAGCAGCAGTTCT
TCCTGGATTGGCACAGATGATAACTGCTGTATCTGCTCAGCAGAATTCTAATTTGATTCCCAAAGTCTTA
ATCCCTGTTAATAGCATTCCCACCTACAATGCTGCATTGGATAACAATCCCCTTTTACTTAACACCTACA
ACAAGTTCCCTTACCCAACAATGTCAGAAATTACAGTTCTTTCTGCTCAAGCAAAATATACAGAGGAACA
GATCAAGATATGGTTTTCAGCCCAACGTTTAAAACATGGTGTTAGTTGGACTCCCGAGGAAGTAGAGGAG
GCAAGAAGGAAACAATTCAATGGAACAGTGCATACTGTACCTCAGACCATAACTGTTATTCCTACACACA
TTTCCACAGGGAGTAATGGTTTACCATCTATTTTACAGACATGCCAAATAGTTGGTCAGCCTGGTCTGGT
CCTTACTCAAGTGGCTGGAACAAACACCTTGCCAGTTACAGCACCTATAGCCTTGACAGTGGCAGGCGTT
CCAAGTCAAAATAATATACAGAAAAGTCAGGTACCTGCTGCTCAGCCTACTGCAGAAACAAAGCCAGCAA
CAGCAGCAGTTCCAACTTCTCAAAGTGTCAAACATGAAACTGCATTGGTAAACCCTGATTCATTTGGCAT
TCGGGCAAAAAAGACAAAAGAGCAACTGGCAGAATTAAAAGTTAGCTACCTTAAAAATCAGTTTCCCCAT
GATTCAGAAATTATCAGACTTATGAAAATAACAGGCCTGACGAAAGGAGAGATTAAAAAATGGTTTAGTG
ACACAAGGTACAACCAGAGAAATTCAAAGAGTAATCAGTGCTTACATCTCAACAATGATTCCTCTACCAC
CATTATTATAGACTCCAGTGATGAAACCACGGAATCCCCAACTGTTGGTACTGCACAGCCTAAGCAATCC
TGGAATCCTTTTCCTGACTTTACTCCCCAAAAGTTTAAAGAGAAAACTGCAGAGCAGCTTCGTGTCCTTC
AGGCAAGTTTTCTCAACAGCTCTGTACTTACAGATGAAGAATTAAATAGGTTAAGGGCACAAACCAAACT
TACCAGAAGAGAAATCGATGCTTGGTTTACAGAGAAGAAGAAATCAAAAGCTTTAAAGGAAGAGAAAATG
GAAATAGATGAAAGTAATGCAGGTAGTTCCAAAGAAGAAGCTGGAGAAACTTCTCCTGCAGATGAATCTG
GTGCACCTAAGTCAGGGAGTACAGGCAAGATATGTAAAAAAACACCTGAGCAGCTGCACATGCTTAAGAG
TGCATTTGTCCGGACACAGTGGCCATCACCAGAAGAGTATGACAAGTTGGCCAAAGAAAGCGGGCTTGCT
AGAACAGACATAGTTAGTTGGTTTGGGGACACCCGTTATGCTTGGAAGAATGGAAACTTGAAATGGTACT
ACTACTATCAGAGCGCCAATTCAAGTAGTATGAATGGTCTGTCTTCCCTTAGGAAAAGAGGGAGAGGGAG
ACCCAAAGGACGGGGAAGAGGAAGACCGCGTGGGCGGCCTAGAGGAAGCAAAAGAATTAACAACTGGGAC
AGGGGACCATCACTCATAAAATTTAAAACTGGAACTGCAATACTTAAGGATTATTACCTGAAGCGCAAGT
TTCTTAATGAGCAAGACCTTGATGAACTTGTTAACAAATCACATATGGGCTATGAGCAGGTCAGAGAGTG
GTTTGCAGAAAGACAGAGAAGATCAGAATTAGGTATAGAATTATTTGAGGAAAATGAGGAGGAAGATGAA
GTTATTGATGACCAGGAAGAGGATGAAGAAGAAACAGATGATAGTGACACTTGGGAACCTCCACGACATG
TGAAACGGAAGCTGTCTAAATCAGATGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206025 protein sequence
Red=Cloning site Green=Tags(s)

MASRRKSTTPCMVLASEQDPDLELISDLDEGPPVLTPVENTRAESISSDEEVHESVDSDNQQNKKVEGGY
ECKYCTFQTPDLNMFTFHVDSEHPNVVLNSSYVCVECNFLTKRYDALSEHNLKYHPGEENFKLTMVKRNN
QTIFEQTINDLTFDGSFVKEENAEQAESTEVSSSGISISKTPIMKMMKNKVENKRIAVHHNSVEDVPEEK
ENEIKPDREEIVENPSSSASESNTSTSIVNRIHPSTASTVVTPAAVLPGLAQMITAVSAQQNSNLIPKVL
IPVNSIPTYNAALDNNPLLLNTYNKFPYPTMSEITVLSAQAKYTEEQIKIWFSAQRLKHGVSWTPEEVEE
ARRKQFNGTVHTVPQTITVIPTHISTGSNGLPSILQTCQIVGQPGLVLTQVAGTNTLPVTAPIALTVAGV
PSQNNIQKSQVPAAQPTAETKPATAAVPTSQSVKHETALVNPDSFGIRAKKTKEQLAELKVSYLKNQFPH
DSEIIRLMKITGLTKGEIKKWFSDTRYNQRNSKSNQCLHLNNDSSTTIIIDSSDETTESPTVGTAQPKQS
WNPFPDFTPQKFKEKTAEQLRVLQASFLNSSVLTDEELNRLRAQTKLTRREIDAWFTEKKKSKALKEEKM
EIDESNAGSSKEEAGETSPADESGAPKSGSTGKICKKTPEQLHMLKSAFVRTQWPSPEEYDKLAKESGLA
RTDIVSWFGDTRYAWKNGNLKWYYYYQSANSSSMNGLSSLRKRGRGRPKGRGRGRPRGRPRGSKRINNWD
RGPSLIKFKTGTAILKDYYLKRKFLNEQDLDELVNKSHMGYEQVREWFAERQRRSELGIELFEENEEEDE
VIDDQEEDEEETDDSDTWEPPRHVKRKLSKSDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007222
ORF Size 2619 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007222.2
RefSeq Size 5139 bp
RefSeq ORF 2622 bp
Locus ID 11244
UniProt ID Q9UKY1, A0A024R9F1
Cytogenetics 8q24.13
Domains homeobox, zf-C2H2
Protein Families Transcription Factors
MW 98.1 kDa
Summary The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 1 of this gene family. In addition to forming homodimers, this protein heterodimerizes with members 2 and 3 of the zinc fingers and homeoboxes family. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 8 open reading frame 76 (C8orf76) gene. provided by RefSeq, Feb 2011
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Documents
Resources
"NM_007222" in other vectors (6)
SKU Description Size Price
RC206025L1 Lenti ORF clone of Human zinc fingers and homeoboxes 1 (ZHX1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,099.00
RC206025L2 Lenti ORF clone of Human zinc fingers and homeoboxes 1 (ZHX1), transcript variant 2, mGFP tagged 10 ug
$1,099.00
RC206025L3 Lenti ORF clone of Human zinc fingers and homeoboxes 1 (ZHX1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,099.00
RC206025L4 Lenti ORF clone of Human zinc fingers and homeoboxes 1 (ZHX1), transcript variant 2, mGFP tagged 10 ug
$1,099.00
RG206025 ZHX1 (tGFP-tagged) - Human zinc fingers and homeoboxes 1 (ZHX1), transcript variant 2 10 ug
$999.00
SC115644 ZHX1 (untagged)-Human zinc fingers and homeoboxes 1 (ZHX1), transcript variant 2 10 ug
$800.00

Plasmid Map for RC206025

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206025
Circular map for RC206025

file-alt Citations

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