PLK4 (NM_014264) Human Tagged ORF Clone
$1,332.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PLK4 |
| Synonyms | MCCRP2; SAK; STK18 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014264 |
| ORF Size | 2910 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014264.5 |
| RefSeq Size | 3870 bp |
| RefSeq ORF | 2913 bp |
| Locus ID | 10733 |
| UniProt ID | O00444 |
| Cytogenetics | 4q28.1 |
| Domains | pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 109 kDa |
| Summary | This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. provided by RefSeq, Jun 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014264" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206015L1 | Lenti ORF clone of Human polo-like kinase 4 (PLK4), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,632.00
|
|
| RC206015L2 | Lenti ORF clone of Human polo-like kinase 4 (PLK4), transcript variant 1, mGFP tagged | 10 ug |
$1,632.00
|
|
| RC206015L3 | Lenti ORF clone of Human polo-like kinase 4 (PLK4), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,632.00
|
|
| RC206015L4 | Lenti ORF clone of Human polo-like kinase 4 (PLK4), transcript variant 1, mGFP tagged | 10 ug |
$1,632.00
|
|
| RG206015 | PLK4 (tGFP-tagged) - Human polo-like kinase 4 (PLK4), transcript variant 1 | 10 ug |
$1,532.00
|
|
| SC115082 | PLK4 (untagged)-Human polo-like kinase 4 (PLK4), transcript variant 1 | 10 ug |
$889.00
|
|
| SC323369 | PLK4 (untagged)-Kinase deficient mutant (K41M) of Human polo-like kinase 4 (Drosophila) (PLK4) | 10 ug |
$889.00
|
|
Citations
TFEB- and TFE3-dependent autophagy activation supports cancer proliferation in the absence of centrosomes
Kao, Su, Huang et al
Autophagy (2022) 18 (12), 2830-2850 DOI: 10.1080/15548627.2022.2051880
Kao, Su, Huang et al
Autophagy (2022) 18 (12), 2830-2850 DOI: 10.1080/15548627.2022.2051880
Cited by 23
Phosphorylation of synucleins by members of the Polo-like kinase family
Mbefo, Paleologou, Boucharaba et al
J Biol Chem (2010) 285 (4), 2807-22 DOI: 10.1074/jbc.M109.081950
Mbefo, Paleologou, Boucharaba et al
J Biol Chem (2010) 285 (4), 2807-22 DOI: 10.1074/jbc.M109.081950
Showing 1-2 of 2 papers.
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