GPX8 (NM_001008397) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GPX8 |
| Synonyms | EPLA847; GPx-8; GSHPx-8; UNQ847 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001008397 |
| ORF Size | 627 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001008397.1, NP_001008398.1 |
| RefSeq Size | 3750 bp |
| RefSeq ORF | 630 bp |
| Locus ID | 493869 |
| UniProt ID | Q8TED1 |
| Cytogenetics | 5q11.2 |
| Protein Families | Transmembrane |
| MW | 23.9 kDa |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001008397" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205924L3 | Lenti ORF clone of Human glutathione peroxidase 8 (putative) (GPX8), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC205924L4 | Lenti ORF clone of Human glutathione peroxidase 8 (putative) (GPX8), mGFP tagged | 10 ug |
$750.00
|
|
| RG205924 | GPX8 (tGFP-tagged) - Human glutathione peroxidase 8 (putative) (GPX8) | 10 ug |
$650.00
|
|
| SC301289 | GPX8 (untagged)-Human glutathione peroxidase 8 (putative) (GPX8) | 10 ug |
$450.00
|
|
| SC321120 | GPX8 (untagged)-Human glutathione peroxidase 8 (putative) (GPX8) | 10 ug |
$450.00
|
|
Citations
Glutathione peroxidase 8 negatively regulates caspase-4/11 to protect against colitis
Hsu, Chou, Chen et al
EMBO Mol Med (2019) 12 (1), e9386 DOI: 10.15252/emmm.201809386
Hsu, Chou, Chen et al
EMBO Mol Med (2019) 12 (1), e9386 DOI: 10.15252/emmm.201809386
AI Summary: OriGene’s cDNA constructs for caspase-4, GPx7, and GPx8 were used as PCR templates to generate expression plasmids, enabling the study of these proteins’ roles in regulating inflammation and colitis.
Snippet: … Materials and Methods Plasmids, antibodies, and reagents Caspase‐4, GPx7, and GPx8 were PCR‐amplified from cDNA constructs (OriGene, Rockville, MD, USA) and cloned into the expression vector pHRSIN (Hsu et al, 2015). … Article Glutathione peroxidase 8 negatively regulates caspase-4/11 to protect against colitis Jye-Lin Hsu 1,2,* , Jen-Wei Chou 3 , Tzu-Fan Chen 1,2 , Jeh-Ting Hsu 4 , Fang-Yi Su 5 , Joung-Liang Lan 6 , Po-Chang Wu;Caspase‐4, GPx7, and GPx8 were PCR‐amplified from cDNA constructs (OriGene, Rockville, MD, USA) and cloned into the expression vector pHRSIN (Hsu et al, 2015). …The N‐terminal truncated GPx8 gene was PCR‐amplified and cloned into Escherichia coli (E. …
Showing 1-1 of 1 papers.
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