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SELH (SELENOH) (NM_170746) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC205870
C11orf31 (Myc-DDK-tagged)-Human chromosome 11 open reading frame 31 (C11orf31), (Note, selenocysteine protein, internal stop codon, see reference data summary)
ORF Plasmid:
  $118.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged ORF Clone
Target Symbol SELH
Synonyms C11orf31; C17orf10; SELH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205870 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCCCGCGGGAGGAAGCGTAAGGCTGAGGCCGCGGTGGTCGCCGTAGCCGAGAAGCGAGAGAAGC
TGGCGAACGGCGGGGAGGGAATGGAGGAGGCGACCGTTGTTATCGAGCATTGCACTAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205870 protein sequence
Red=Cloning site Green=Tags(s)

MAPRGRKRKAEAAVVAVAEKREKLANGGEGMEEATVVIEHCTS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_170746
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 1294 bp
RefSeq ORF 369 bp
Locus ID 280636
UniProt ID Q8IZQ5
Cytogenetics 11q12.1
Summary This gene encodes a nucleolar protein, which belongs to the SelWTH family. It functions as an oxidoreductase, and has been shown to protect neurons against UVB-induced damage by inhibiting apoptotic cell death pathways, promote mitochondrial biogenesis and mitochondrial function, and suppress cellular senescence through genome maintenance and redox regulation. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, May 2016
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"NM_170746" in other vectors (2)
SKU Description Size Price
RG205870 C11orf31 (GFP-tagged) - Human chromosome 11 open reading frame 31 (C11orf31), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) 10 ug
$489.00
SC100672 C11orf31 (untagged)-Human chromosome 11 open reading frame 31 (C11orf31) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) 10 ug
$462.00

Plasmid Map for RC205870

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Cloning scheme for RC205870
Circular map for RC205870

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