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SLC35E3 (NM_018656) Human Tagged ORF Clone

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SKU
RC205831
SLC35E3 (Myc-DDK-tagged)-Human solute carrier family 35, member E3 (SLC35E3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC35E3
Synonyms BLOV1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205831 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATTGCTGGTGGACCGAGTGCGGGGCCACTGGCGAATCGCCGCCGGGCTCCTGTTCAACCTGCTGG
TGTCCATCTGCATTGTGTTCCTCAACAAATGGATTTATGTGTACCACGGCTTCCCCAACATGAGCCTGAC
CCTGGTGCACTTCGTGGTCACCTGGCTGGGCTTGTATATCTGCCAGAAGCTGGACATCTTTGCCCCCAAA
AGTCTGCCGCCCTCCAGGCTCCTCCTCCTGGCCCTCAGCTTCTGTGGCTTTGTGGTCTTCACTAACCTTT
CTCTGCAGAACAACACCATAGGCACCTATCAGCTGGCCAAGGCCATGACCACGCCGGTGATCATAGCCAT
CCAGACCTTCTGCTACCAGAAAACCTTCTCCACCAGAATCCAGCTCACGCTGATTCCTATAACTTTAGGT
GTAATCCTAAATTCTTATTACGATGTGAAGTTTAATTTCCTTGGAATGGTGTTTGCTGCTCTTGGTGTTT
TAGTTACATCCCTTTATCAAGTGTGGGTAGGAGCCAAACAGCATGAATTACAAGTGAACTCAATGCAGCT
GCTGTACTACCAGGCTCCGATGTCATCTGCCATGTTGCTGGTTGCTGTGCCCTTCTTTGAGCCAGTGTTT
GGAGAAGGAGGAATATTTGGTCCCTGGTCAGTTTCTGCTTTGCTTATGGTGCTGCTATCTGGAGTAATAG
CTTTCATGGTGAACTTATCAATTTATTGGATCATTGGGAACACTTCACCTGTCACCTATAACATGTTCGG
ACACTTCAAGTTCTGCATTACTTTATTCGGAGGATATGTTTTATTTAAGGATCCACTGTCCATTAATCAG
GCCCTTGGCATTTTATGTACATTATTTGGCATTCTCGCCTATACCCACTTTAAGCTCAGTGAACAGGAAG
GAAGTAGGAGTAAACTGGCACAACGTCCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205831 protein sequence
Red=Cloning site Green=Tags(s)

MALLVDRVRGHWRIAAGLLFNLLVSICIVFLNKWIYVYHGFPNMSLTLVHFVVTWLGLYICQKLDIFAPK
SLPPSRLLLLALSFCGFVVFTNLSLQNNTIGTYQLAKAMTTPVIIAIQTFCYQKTFSTRIQLTLIPITLG
VILNSYYDVKFNFLGMVFAALGVLVTSLYQVWVGAKQHELQVNSMQLLYYQAPMSSAMLLVAVPFFEPVF
GEGGIFGPWSVSALLMVLLSGVIAFMVNLSIYWIIGNTSPVTYNMFGHFKFCITLFGGYVLFKDPLSINQ
ALGILCTLFGILAYTHFKLSEQEGSRSKLAQRP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018656
ORF Size 939 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018656.5
RefSeq Size 2347 bp
RefSeq ORF 942 bp
Locus ID 55508
UniProt ID Q7Z769, A0A024RB82
Cytogenetics 12q15
Protein Families Transmembrane
MW 35.1 kDa
Summary Putative transporter.UniProtKB/Swiss-Prot Function
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"NM_018656" in other vectors (4)
SKU Description Size Price
RC205831L3 Lenti ORF clone of Human solute carrier family 35, member E3 (SLC35E3), Myc-DDK-tagged 10 ug
$600.00
RC205831L4 Lenti ORF clone of Human solute carrier family 35, member E3 (SLC35E3), mGFP tagged 10 ug
$600.00
RG205831 SLC35E3 (tGFP-tagged) - Human solute carrier family 35, member E3 (SLC35E3) 10 ug
$489.00 $500.00
SC113413 SLC35E3 (untagged)-Human solute carrier family 35, member E3 (SLC35E3) 10 ug
$300.00

Plasmid Map for RC205831

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205831
Circular map for RC205831

file-alt Citations

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