Alkaline Phosphatase (ALPL) (NM_000478) Human Tagged ORF Clone
SKU
RC205692
ALPL (Myc-DDK-tagged)-Human alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1
$488.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Alkaline Phosphatase |
| Synonyms | AP-TNAP; APTNAP; HOPS; HPPA; HPPC; HPPI; HPPO; TNALP; TNAP; TNSALP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000478 |
| ORF Size | 1572 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000478.5 |
| RefSeq Size | 2606 bp |
| RefSeq ORF | 1575 bp |
| Locus ID | 249 |
| UniProt ID | P05186, A0A024RAB4 |
| Cytogenetics | 1p36.12 |
| Domains | alk_phosphatase |
| Protein Families | Druggable Genome |
| Protein Pathways | Folate biosynthesis, Metabolic pathways |
| MW | 57.3 kDa |
| Summary | This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. provided by RefSeq, Oct 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000478" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205692L1 | Lenti ORF clone of Human alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1, Myc-DDK-tagged | 10 ug |
$788.00
|
|
| RC205692L2 | Lenti ORF clone of Human alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1, mGFP tagged | 10 ug |
$788.00
|
|
| RC205692L3 | Lenti ORF clone of Human alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1, Myc-DDK-tagged | 10 ug |
$788.00
|
|
| RC205692L4 | Lenti ORF clone of Human alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1, mGFP tagged | 10 ug |
$788.00
|
|
| RG205692 | ALPL (tGFP-tagged) - Human alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1 | 10 ug |
$489.00
$688.00
|
|
| SC107943 | ALPL (untagged)-Human alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1 | 10 ug |
$489.00
|
|
| SC320807 | ALPL (untagged)-Human alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1 | 10 ug |
$489.00
|
|
Citations
The ALPL Gene Variant Project: Results of the first 100 reclassified variants
Farman, Malli, Rehder et al
JBMR Plus (2025)
Farman, Malli, Rehder et al
JBMR Plus (2025)
AI Summary: OriGene's ALPL WT plasmid (#RC205692) was used as a control and for generating mutant constructs in transfection experiments to assess the functional impact of different ALPL gene variants in this study.
Snippet: Transfection (lipofectamine 3000, Invitrogen , #L3000015) involved using 100% ALPL WT plasmid (Origene, #RC205692) as control, or 100% mutant plasmid (in the following referred to as “single transfection”), or a mix of 50% WT / 50% mutant plasmid (in the following referred to as “co-transfection”)
The Global ALPL gene variant classification project: Dedicated to deciphering variants
Farman, Rehder, Malli et al
Bone (2023) 178, 116947 DOI: 10.1016/j.bone.2023.116947
Farman, Rehder, Malli et al
Bone (2023) 178, 116947 DOI: 10.1016/j.bone.2023.116947
Cited by 67
AI Summary: OriGene's ALPL plasmid (catalog #RC205692) was used as the template for site-directed mutagenesis to generate and functionally assess ALPL gene variants in the study.
Snippet: Background Hypophosphatasia (HPP) is an inherited multisystem disorder predominantly affecting the mineralization of bones and teeth. HPP is caused by pathogenic Save;To do so, site directed mutagenesis is performed according to the manual instruction (Agilent, #200522) on the ALPL plasmid (OriGene, #RC205692)
Activation of nuclear factor-kappa B by TNF promotes nucleus pulposus mineralization through inhibition of ANKH and ENPP1
Krzyzanowska, Frawley, Damle et al
Sci Rep (2021) 11 (1), 8271 DOI: 10.1038/s41598-021-87665-2
Krzyzanowska, Frawley, Damle et al
Sci Rep (2021) 11 (1), 8271 DOI: 10.1038/s41598-021-87665-2
Cited by 10
AI Summary: In this study, OriGene products—specifically TNAP, TNF, and ANKH gene constructs—were used to manipulate gene expression in nucleus pulposus cells, enabling the researchers to investigate how TNF-induced NF-κB activation promotes mineralization by inhibiting ANKH and ENPP1.
Snippet: ion, in single or double-transduced bNPs respectively. pMXs-IRES-Bsd, pMX-IRES-Neo, pMXs-U6-Puro were purchased from Cell Biolabs, Inc, San Diego, CA, whereas TNAP, TNF, ANKH genes were obtained from OriGene Technologies, Inc., Rockville, MD. Cell culture Naïve and transduced bNP cells were seeded at 130 × 103 cells/cm2 in Falcon 12-well plates (BD Biosciences). Cells were maintained in mi
Showing 1-3 of 3 papers.
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