Myozenin 2 (MYOZ2) (NM_016599) Human Tagged ORF Clone

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SKU
RC205616
MYOZ2 (Myc-DDK-tagged)-Human myozenin 2 (MYOZ2)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Myozenin 2
Synonyms C4orf5; CMH16; CS-1; FATZ-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205616 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTATCACATAATACTATGATGAAGCAGAGAAAACAGCAAGCAACAGCCATCATGAAGGAAGTCCATG
GAAATGATGTTGATGGCATGGACCTGGGCAAAAAGGTCAGCATCCCCAGAGACATCATGTTGGAAGAATT
ATCCCATCTCAGTAACCGTGGTGCCAGGCTATTTAAGATGCGTCAAAGAAGATCTGACAAATACACATTT
GAAAATTTCCAGTATCAATCTAGAGCGCAAATAAATCACAGTATTGCTATGCAGAATGGGAAAGTGGATG
GAAGTAACTTGGAAGGTGGTTCGCAGCAAGCCCCCTTGACTCCTCCCAACACCCCAGATCCACGAAGCCC
TCCAAATCCAGACAACATTGCTCCAGGATATTCTGGACCACTGAAGGAAATTCCTCCTGAAAAATTCAAC
ACCACAGCTGTCCCTAAGTACTATCAATCTCCCTGGGAACAAGCCATTAGCAATGATCCGGAGCTTTTAG
AGGCTTTATATCCTAAACTTTTCAAGCCTGAAGGAAAGGCAGAACTGCCTGATTACAGGAGCTTTAACAG
GGTTGCCACACCATTTGGAGGTTTTGAAAAAGCATCAAGAATGGTTAAATTTAAAGTTCCAGATTTTGAG
CTACTATTGCTAACAGATCCCAGGTTTATGTCCTTTGTCAATCCCCTTTCTGGCAGACGGTCCTTTAATA
GGACTCCTAAGGGATGGATATCTGAGAATATTCCTATAGTGATAACAACCGAACCTACAGATGATACCAC
TGTACCAGAATCAGAAGACCTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205616 protein sequence
Red=Cloning site Green=Tags(s)

MLSHNTMMKQRKQQATAIMKEVHGNDVDGMDLGKKVSIPRDIMLEELSHLSNRGARLFKMRQRRSDKYTF
ENFQYQSRAQINHSIAMQNGKVDGSNLEGGSQQAPLTPPNTPDPRSPPNPDNIAPGYSGPLKEIPPEKFN
TTAVPKYYQSPWEQAISNDPELLEALYPKLFKPEGKAELPDYRSFNRVATPFGGFEKASRMVKFKVPDFE
LLLLTDPRFMSFVNPLSGRRSFNRTPKGWISENIPIVITTEPTDDTTVPESEDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016599
ORF Size 792 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016599.5
RefSeq Size 2604 bp
RefSeq ORF 795 bp
Locus ID 51778
UniProt ID Q9NPC6
Cytogenetics 4q26
MW 29.9 kDa
Summary The protein encoded by this gene belongs to a family of sarcomeric proteins that bind to calcineurin, a phosphatase involved in calcium-dependent signal transduction in diverse cell types. These family members tether calcineurin to alpha-actinin at the z-line of the sarcomere of cardiac and skeletal muscle cells, and thus they are important for calcineurin signaling. Mutations in this gene cause cardiomyopathy familial hypertrophic type 16, a hereditary heart disorder. provided by RefSeq, Aug 2011
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Documents
Resources
"NM_016599" in other vectors (4)
SKU Description Size Price
RC205616L3 Lenti ORF clone of Human myozenin 2 (MYOZ2), Myc-DDK-tagged 10 ug
$600.00
RC205616L4 Lenti ORF clone of Human myozenin 2 (MYOZ2), mGFP tagged 10 ug
$600.00
RG205616 MYOZ2 (tGFP-tagged) - Human myozenin 2 (MYOZ2) 10 ug
$489.00 $500.00
SC114152 MYOZ2 (untagged)-Human myozenin 2 (MYOZ2) 10 ug
$300.00

Plasmid Map for RC205616

Download sequence for

Cloning scheme for RC205616

Circular map for RC205616

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