PDZD8 (NM_173791) Human Tagged ORF Clone
$1,506.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PDZD8 |
| Synonyms | PDZK8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_173791 |
| ORF Size | 3462 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_173791.3, NP_776152.1 |
| RefSeq Size | 3853 bp |
| RefSeq ORF | 3465 bp |
| Locus ID | 118987 |
| UniProt ID | Q8NEN9 |
| Cytogenetics | 10q25.3-q26.11 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 128.6 kDa |
| Summary | Molecular tethering protein that connects endoplasmic reticulum and mitochondria membranes (PubMed:29097544). PDZD8-dependent endoplasmic reticulum-mitochondria membrane tethering is essential for endoplasmic reticulum-mitochondria Ca(2+) transfer (PubMed:29097544). In neurons, involved in the regulation of dendritic Ca(2+) dynamics by regulating mitochondrial Ca(2+) uptake in neurons (PubMed:29097544). Plays an indirect role in the regulation of cell morphology and cytoskeletal organization (PubMed:21834987). May inhibit herpes simplex virus 1 infection at an early stage (PubMed:21549406).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_173791" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205601L3 | Lenti-ORF clone of PDZD8 (Myc-DDK-tagged)-Human PDZ domain containing 8 (PDZD8) | 10 ug |
$1,806.00
|
|
| RC205601L4 | Lenti-ORF clone of PDZD8 (mGFP-tagged)-Human PDZ domain containing 8 (PDZD8) | 10 ug |
$1,806.00
|
|
| RG205601 | PDZD8 (tGFP-tagged) - Human PDZ domain containing 8 (PDZD8) | 10 ug |
$1,706.00
|
|
| SC107070 | PDZD8 (untagged)-Human PDZ domain containing 8 (PDZD8) | 10 ug |
$1,508.00
|
|
Citations
PDZD8-mediated lipid transfer at contacts between the ER and late endosomes/lysosomes is required for neurite outgrowth
Gao, Xiong, Chu et al
J Cell Sci (2022) 135 (5) DOI: 10.1242/jcs.255026
Gao, Xiong, Chu et al
J Cell Sci (2022) 135 (5) DOI: 10.1242/jcs.255026
Cited by 68
AI Summary: OriGene’s Human PDZD8-Myc-Flag plasmid (RC205601) was used as the source for generating various PDZD8 constructs—including PDZD8-GFP, truncated mutants, and PDZD8-Halo—that enabled the study of PDZD8’s role in lipid transfer and neurite outgrowth.
Snippet: Skip to Main Content Advertisement;Human PDZD8-Myc-Flag was purchased from Origene (RC205601). PDZD8–GFP and all of the truncated PDZD8 mutants used in this study were generated by PCR amplifying human PDZD8-Myc-Flag (Origene, RC205601) and cloning it into mGFP-N1 (Addgene 54767). … PDZD8-Halo was generated by inserting PDZD8 (RC205601) into Halo-N1, generated by replacing GFP with Halo in mGFP-N1 vector. pLVX-EF1α-PDZD8-GFP was generated by inserting PDZD8 into pLVX-EF1α-acGFP-N1 vector (Clontech, 631983). …
VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions
Du, Wang, Xiong et al
Mol Biol Cell (2021) 32 (16), 1474-1486 DOI: 10.1091/mbc.E21-03-0097
Du, Wang, Xiong et al
Mol Biol Cell (2021) 32 (16), 1474-1486 DOI: 10.1091/mbc.E21-03-0097
AI Summary: OriGene products, specifically the VPS13D shRNA (OriGene, #TL308406) and VPS13D cDNA (OriGene, #RC217559), were used in this study to manipulate VPS13D expression levels in cells, enabling the authors to investigate the role of VPS13D in regulating ER-mitochondria interactions.
VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions
Du, Wang, Xiong et al
MBoC (2021) 32 (16), mbc.E21-03-0097 DOI: 10.1091/mbc.E21-03-0097
Du, Wang, Xiong et al
MBoC (2021) 32 (16), mbc.E21-03-0097 DOI: 10.1091/mbc.E21-03-0097
AI Summary: OriGene’s human VPS13D-myc-flag plasmid (RC205601) was used as a template to generate truncated VPS13D mutants, enabling the study of VPS13D’s role in regulating ER-mitochondria interactions.
Snippet: G (12259) were bought from Addgene. All of truncated mutants of VPS13D or p97 used in this study were generated by PCR using human VPS13D-myc-flag (Origene, RC205601) or p97-GFP (addgene, 23971) as templates and cloned it into mGFP-N1 (addgene 54767
PDZD8 is a novel Gag-interacting factor that promotes retroviral infection
Henning, Morham, Goff et al
J Virol (2010) 84 (17), 8990-5 DOI: 10.1128/JVI.00843-10
Henning, Morham, Goff et al
J Virol (2010) 84 (17), 8990-5 DOI: 10.1128/JVI.00843-10
AI Summary: OriGene products, specifically the PDZD8 Myc-DDK tagged expression plasmid (RC209307) and PDZD8 shRNA plasmids (TL313606), were used in this study to overexpress and knockdown PDZD8 in cells, enabling the authors to demonstrate PDZD8’s role in promoting HIV-1 infection and its interaction with the viral Gag protein.
Showing 1-4 of 4 papers.
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