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ACTL7A (NM_006687) Human Tagged ORF Clone

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SKU
RC205558
ACTL7A (Myc-DDK-tagged)-Human actin-like 7A (ACTL7A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ACTL7A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205558 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGCTCCACCAGCAGCAATCATGGGGGATGGGCCCACCAAGAAGGTGGGCAACCAGGCCCCCCTGC
AGACACAGGCCCTCCAGACTGCCTCTTTAAGGGATGGCCCGGCGAAGCGGGCCGTGTGGGTCCGCCATAC
GAGTTCAGAGCCACAAGAACCTACTGAATCAAAGGCAGCCAAGGAGAGGCCCAAGCAGGAGGTGACCAAA
GCAGTGGTCGTGGACCTGGGCACTGGCTACTGTAAATGTGGCTTTGCCGGCCTGCCAAGACCCACCCACA
AGATCTCAACAACGGTGGGCAAGCCCTACATGGAGACCGCCAAGACTGGGGATAATCGCAAGGAGACATT
CGTGGGGCAGGAACTCAACAACACAAACGTTCATCTCAAGCTGGTTAACCCTCTGCGACATGGCATCATC
GTGGACTGGGATACAGTGCAGGATATCTGGGAATATCTCTTCCGACAAGAGATGAAGATCGCCCCGGAGG
AGCATGCGGTCTTGGTTTCAGACCCGCCACTGAGCCCACACACCAACAGAGAGAAATATGCTGAAATGCT
GTTTGAAGCCTTCAACACCCCTGCAATGCACATCGCCTACCAGTCGCGCCTGTCCATGTACTCCTATGGA
AGGACCTCCGGCCTGGTGGTGGAGGTGGGCCATGGCGTGTCCTACGTGGTCCCCATCTACGAGGGTTATC
CTTTGCCCAGCATCACCGGAAGGCTGGACTACGCGGGCTCTGACCTGACAGCCTACCTGCTGGGCCTGCT
GAACAGTGCGGGGAACGAATTCACCCAGGACCAGATGGGCATCGTGGAGGACATCAAGAAGAAATGCTGC
TTTGTGGCCCTGGATCCCATTGAGGAGAAGAAAGTCCCTCTCAGTGAGCATACGATCCGCTACGTGCTGC
CGGATGGGAAGGAGATTCAGCTGTGCCAGGAACGGTTCCTCTGCTCGGAGATGTTCTTCAAGCCATCTCT
CATCAAGTCCATGCAGCTGGGCCTCCACACCCAAACCGTGTCCTGCCTTAACAAGTGTGACATCGCCCTC
AAACGGGACCTCATGGGGAACATCCTGCTCTGCGGGGGCAGCACGATGCTCAGTGGCTTCCCTAACCGTC
TGCAGAAGGAGCTAAGCAGCATGTGTCCCAATGACACCCCGCAGGTAAACGTGCTGCCTGAAAGAGACAG
TGCCGTGTGGACCGGTGGCTCCATCCTGGCCTCACTTCAGGGTTTCCAACCATTATGGGTCCACCGCTTT
GAGTACGAGGAACACGGGCCTTTCTTCCTCTACAGAAGGTGCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205558 protein sequence
Red=Cloning site Green=Tags(s)

MWAPPAAIMGDGPTKKVGNQAPLQTQALQTASLRDGPAKRAVWVRHTSSEPQEPTESKAAKERPKQEVTK
AVVVDLGTGYCKCGFAGLPRPTHKISTTVGKPYMETAKTGDNRKETFVGQELNNTNVHLKLVNPLRHGII
VDWDTVQDIWEYLFRQEMKIAPEEHAVLVSDPPLSPHTNREKYAEMLFEAFNTPAMHIAYQSRLSMYSYG
RTSGLVVEVGHGVSYVVPIYEGYPLPSITGRLDYAGSDLTAYLLGLLNSAGNEFTQDQMGIVEDIKKKCC
FVALDPIEEKKVPLSEHTIRYVLPDGKEIQLCQERFLCSEMFFKPSLIKSMQLGLHTQTVSCLNKCDIAL
KRDLMGNILLCGGSTMLSGFPNRLQKELSSMCPNDTPQVNVLPERDSAVWTGGSILASLQGFQPLWVHRF
EYEEHGPFFLYRRCF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006687
ORF Size 1305 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006687.4
RefSeq Size 1528 bp
RefSeq ORF 1308 bp
Locus ID 10881
UniProt ID Q9Y615, A0A140VK03
Cytogenetics 9q31.3
MW 48.6 kDa
Summary The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene (ACTL7A), and related gene, ACTL7B, are intronless, and are located approximately 4 kb apart in a head-to-head orientation within the familial dysautonomia candidate region on 9q31. Based on mutational analysis of the ACTL7A gene in patients with this disorder, it was concluded that it is unlikely to be involved in the pathogenesis of dysautonomia. The ACTL7A gene is expressed in a wide variety of adult tissues, however, its exact function is not known. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006687" in other vectors (4)
SKU Description Size Price
RC205558L3 Lenti ORF clone of Human actin-like 7A (ACTL7A), Myc-DDK-tagged 10 ug
$757.00
RC205558L4 Lenti ORF clone of Human actin-like 7A (ACTL7A), mGFP tagged 10 ug
$757.00
RG205558 ACTL7A (tGFP-tagged) - Human actin-like 7A (ACTL7A) 10 ug
$489.00 $657.00
SC122758 ACTL7A (untagged)-Human actin-like 7A (ACTL7A) 10 ug
$457.00

Plasmid Map for RC205558

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205558
Circular map for RC205558

file-alt Citations

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