HCG3 (DNAJB3) (NM_001001394) Human Tagged ORF Clone
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | HCG3 |
| Synonyms | HCG3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001001394 |
| ORF Size | 435 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001001394.4 |
| RefSeq Size | 1266 bp |
| RefSeq ORF | 438 bp |
| Locus ID | 414061 |
| UniProt ID | Q8WWF6 |
| Cytogenetics | 2q37.1 |
| MW | 16.6 kDa |
| Summary | May operate as a co-chaperone of the male germ cell- and haploid stage-specific Hsp70 proteins.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001001394" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205526L1 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC205526L2 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3), mGFP tagged | 10 ug |
$450.00
|
|
| RC205526L3 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC205526L4 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3), mGFP tagged | 10 ug |
$450.00
|
|
| RG205526 | DNAJB3 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3) | 10 ug |
$489.00
|
|
| SC300175 | DNAJB3 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3) | 10 ug |
$165.00
|
|
Citations
DNAJB3 attenuates ER stress through direct interaction with AKT
Islam, Diane, Khattab et al
PLoS One (2023) 18 (8), e0290340 DOI: 10.1371/journal.pone.0290340
Islam, Diane, Khattab et al
PLoS One (2023) 18 (8), e0290340 DOI: 10.1371/journal.pone.0290340
Cited by 3
AI Summary: OriGene products contributed to this study by providing the expression vectors pCMV-DNAJB3 (#RC205526), pCMV-AKT1 (#R220257), and the pCMV empty vector (#pCMV6KN), which were used for the expression and functional analysis of DNAJB3 and AKT1 in investigating their roles in ER stress and glucose transport.
Snippet: constructs, primers and silencing RNA (siRNA) The expression vector encoding human DNAJB3 (pCMV-DNAJB3;#RC205526), AKT (pCMV-AKT1;#R220257) and the pCMV empty vector (#pCMV6KN) were purchased from OriGene (OriGene Technologies, Inc, Rockville, MD). Plasmids encoding hDNAJB3 as a C-terminal fusion with GST (pGK-DNAJB3) and 6xHIS residues (pH6K-DNAJB3) were made by digesting pCMV-DNAJB3 with BglI
DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation
Arredouani, Diane, Khattab et al
Sci Rep (2019) 9 (1), 4772 DOI: 10.1038/s41598-019-41244-8
Arredouani, Diane, Khattab et al
Sci Rep (2019) 9 (1), 4772 DOI: 10.1038/s41598-019-41244-8
AI Summary: OriGene products pCMV-DNAJB3 and pCMV-HSPA1A plasmids were used to overexpress human DNAJB3 and HSP-72 in cells, enabling the study of their effects on metabolic stress and glucose uptake in this research.
Snippet: All the cells were used before the 25 th passage. Plasmids and silencing RNA. pCMV-DNAJB3 and pCMV-HSPA1A plasmids were purchased from Origene (Origene Technologies, Inc., Rockvile, MD). They encode the human DNAJB3 and HSP-72, respectively
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
