HORMAD2 (NM_152510) Human Tagged ORF Clone

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SKU
RC205453
HORMAD2 (Myc-DDK-tagged)-Human HORMA domain containing 2 (HORMAD2)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HORMAD2
Synonyms CT46.2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205453 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTGCTCAGCTTTCTCACTGCATCACAATACACAAGGCTTCTAAGGAAACAGTTTTCCCATCCC
AAATCACTAATGAGCATGAGTCATTGAAAATGGTGAAGAAACTTTTTGCTACTTCCATCTCATGTATAAC
ATACCTAAGGGGCCTGTTTCCAGAGAGCTCTTATGGAGAACGCCATTTGGATGACCTCAGTTTAAAAATC
CTCCGAGAAGATAAAAAATGTCCCGGGTCACTGCATATTATCAGATGGATTCAAGGTTGTTTTGATGCTT
TGGAAAAGAGATACCTACGTATGGCAGTACTGACACTTTACACAGATCCCATGGGATCTGAGAAGGTGAC
TGAGATGTACCAGTTCAAATTCAAATACACGAAAGAAGGAGCCACTATGGATTTTGACAGTCATAGCAGC
AGTACAAGCTTTGAAAGTGGAACAAACAATGAAGATATTAAGAAAGCCAGTGTTCTACTGATCCGTAAAT
TGTATATACTGATGCAGGACCTTGAGCCACTTCCTAATAATGTTGTACTTACTATGAAACTCCACTACTA
TAATGCAGTGACCCCACATGATTACCAACCCCTCGGTTTTAAAGAAGGGGTAAATTCACACTTCCTGCTG
TTTGACAAGGAGCCTATCAACGTGCAAGTGGGATTTGTCTCCACTGGCTTTCATAGCATGAAAGTAAAAG
TCATGACAGAGGCTACAAAAGTGATTGATTTGGAGAACAATCTGTTTCGGGAGAACAGCACTACTGAGAT
CGCCCATCAGGGTCTAGACTGTGATGAGGAAGAAGAATGCAATGACCATATTCAAAGAATGAATTTTGTG
TGCAGTCAGCAAAGTTCTGAGTGCTCCAGGAAGAAGAGGAAGGTCAGTGAACCAGTGAAGGTCTTCATCC
CTAACAGAAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205453 protein sequence
Red=Cloning site Green=Tags(s)

MATAQLSHCITIHKASKETVFPSQITNEHESLKMVKKLFATSISCITYLRGLFPESSYGERHLDDLSLKI
LREDKKCPGSLHIIRWIQGCFDALEKRYLRMAVLTLYTDPMGSEKVTEMYQFKFKYTKEGATMDFDSHSS
STSFESGTNNEDIKKASVLLIRKLYILMQDLEPLPNNVVLTMKLHYYNAVTPHDYQPLGFKEGVNSHFLL
FDKEPINVQVGFVSTGFHSMKVKVMTEATKVIDLENNLFRENSTTEIAHQGLDCDEEEECNDHIQRMNFV
CSQQSSECSRKKRKVSEPVKVFIPNRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152510
ORF Size 921 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152510.4
RefSeq Size 1909 bp
RefSeq ORF 924 bp
Locus ID 150280
UniProt ID Q8N7B1
Cytogenetics 22q12.2
MW 35.3 kDa
Summary Essential for synapsis surveillance during meiotic prophase via the recruitment of ATR activity. Plays a key role in the male mid-pachytene checkpoint and the female meiotic prophase checkpoint: required for efficient build-up of ATR activity on unsynapsed chromosome regions, a process believed to form the basis of meiotic silencing of unsynapsed chromatin (MSUC) and meiotic prophase quality control in both sexes. Required for the DNA double-strand break-independent, BRCA1-dependent activation of ATR on the sex chromosomes that is essential for normal sex body formation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_152510" in other vectors (4)
SKU Description Size Price
RC205453L3 Lenti ORF clone of Human HORMA domain containing 2 (HORMAD2), Myc-DDK-tagged 10 ug
$600.00
RC205453L4 Lenti ORF clone of Human HORMA domain containing 2 (HORMAD2), mGFP tagged 10 ug
$600.00
RG205453 HORMAD2 (tGFP-tagged) - Human HORMA domain containing 2 (HORMAD2) 10 ug
$489.00 $500.00
SC123255 HORMAD2 (untagged)-Human HORMA domain containing 2 (HORMAD2) 10 ug
$300.00

Plasmid Map for RC205453

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Cloning scheme for RC205453

Circular map for RC205453

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